File: pAc5.1/V5-His B 24-0571
Mode: Normal              1 -    5379
Table: DNASIS#  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatI        AGG!CCT                  1   2776
AatII       GACGT!C                  4    524    986   1569   5316
Acc65I      G!GTACC                  1   2580
AccI        GT!MKAC                  2   1210   3049
AccIII      T!CCGGA                  0
AcyI        GR!CGYC                  7    521    983   1390   1566   1655
                                         4931   5313
AfaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2582   2709   4874
AflI        G!GWCC                   6   2258   2468   3196   3284   4532
                                         4754
AflII       C!TTAAG                  1   1935
AflIII      A!CRYGT                  4    169    596   2113   2704
AgeI        A!CCGGT                  1   2710
AhaI        CC!SGG                   6    427    428   2571   3881   4577
                                         4928
AhaII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4931   5313
AhaIII      TTT!AAA                  6   1170   2740   3001   4260   4279
                                         4971
Alw44I      G!TGCAC                  2   3815   5061
AlwI        GGATCNNNN!               6   2581   2813   3502   4150   4247
                                         5027
AlwI        !NNNNNGATCC              6   2800   4062   4148   4246   4710
                                         5031
AlwNI       CAGNNN!CTG               2   1577   3917
AocI        CC!TNAGG                 0
AocII       GDGCH!C                  5   2653   2784   3819   4980   5065
Aor51HI     AGC!GCT                  2   2438   3174
AosI        TGC!GCA                  2   1681   4616
AosII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4931   5313
ApaI        GGGCC!C                  1   2653
ApaLI       G!TGCAC                  2   3815   5061
ApoI        R!AATTY                  5    508    581   2329   2604   2885
ApyI        CC!WGG                   5    385   3457   3529   3650   3663
AquI        C!YCGRG                  2    426   2637
AscI        GG!CGCGCC                0
AseI        AT!TAAT                  2     12   4566
AsnI        AT!TAAT                  2     12   4566
Asp700I     GAANN!NNTTC              4     13    802   1873   4993
Asp718I     G!GTACC                  1   2580
AspHI       GWGCW!C                  4   2784   3819   4980   5065
AspI        GACN!NNGTC               0
AsuI        G!GNCC                  13    154    430   2258   2468   2649
                                         2650   3196   3284   4436   4515
                                         4532   4754   5370
AsuII       TT!CGAA                  1   2660
AvaI        C!YCGRG                  2    426   2637
AvaII       G!GWCC                   6   2258   2468   3196   3284   4532
                                         4754
AvaIII      ATGCA!T                  1   1407
AviII       TGC!GCA                  2   1681   4616
AvrI        !CYCGRG                  2    425   2636
AvrII       C!CTAGG                  0
AxyI        CC!TNAGG                 0
BalI        TGG!CCA                  2    181    348
BamHI       G!GATCC                  1   2805
BanI        G!GYRCC                  4   1389   2580   3163   4342
BanII       GRGCY!C                  2   2653   2784
BanIII      AT!CGAT                  1   1709
BbeI        GGCGC!C                  1   1393
BbiII/AcyI  GR!CGYC                  7    521    983   1390   1566   1655
                                         4931   5313
BbrPI       CAC!GTG                  0
BbsI        !NNNNNNGTCTTC            3    254   3127   5372
BbsI        GAAGACNN!                0
BbvI        GCAGCNNNNNNNN!           9    943   1254   1636   1916   3182
                                         3920   3923   4129   4823
BbvI        !NNNNNNNNNNNNGCTGC       7    127   1828   2449   2911   3830
                                         4432   4621
BbvII       GAAGACNN!                0
BbvII       !NNNNNNGTCTTC            3    254   3127   5372
BcefI       ACGGCNNNNNNNNNNNN!       3   1338   1871   4003
BcefI       !NNNNNNNNNNNNNGCCGT      2   1111   1901
BclI        T!GATCA                  4   1821   2749   2788   3042
BcnI        CC!SGG                   6    427    428   2571   3881   4577
                                         4928
BfaI        C!TAG                   10    309   1085   1960   1975   2588
                                         2644   2852   3996   4249   4584
BfrI        C!TTAAG                  1   1935
BglI        GCCNNNN!NGGC             2   3332   4514
BglII       A!GATCT                  1    133
BinI        !NNNNNGATCC              6   2800   4062   4148   4246   4710
                                         5031
BinI        GGATCNNNN!               6   2581   2813   3502   4150   4247
                                         5027
BlnI        C!CTAGG                  0
BmyI        GDGCH!C                  5   2653   2784   3819   4980   5065
Bpu1102I    GC!TNAGC                 1   2440
BsaAI       YAC!GTR                  0
BsaBI       GATNN!NNATC              2   1770   3041
BsaHI       GR!CGYC                  7    521    983   1390   1566   1655
                                         4931   5313
BsaI        GGTCTCN!                 1   2698
BsaI        !NNNNNGAGACC             2   2559   4455
BsaJI       C!CNNGG                  7    426    427   2262   2569   2653
                                         2688   3661
BseAI       T!CCGGA                  0
BsePI       !GCGCGC                  0
BsiEI       CGRY!CG                  9    392   1331   2634   2801   3053
                                         3339   3841   4764   4913
BsiWI       C!GTACG                  1   1097
BsiYI       CCNNNNN!NNGG            14    384    427   1052   2321   2377
                                         2575   2576   2577   2689   3093
                                         3523   3541   3707   3986
BslI        CCNNNNN!NNGG            14    384    427   1052   2321   2377
                                         2575   2576   2577   2689   3093
                                         3523   3541   3707   3986
BsmAI       !NNNNNGAGAC              3   1540   2559   4455
BsmAI       GTCTCN!                  6    483   1045   1662   1674   2698
                                         5231
BsmI        NG!CATTC                 1   2954
BsmI        GAATGCN!                 4    285    504   1407   2861
Bsp1286I    GDGCH!C                  5   2653   2784   3819   4980   5065
BspDI       AT!CGAT                  1   1709
BspEI       T!CCGGA                  0
BspHI       T!CATGA                  4     56   4221   5229   5334
BspMI       ACCTGCNNNN!              0
BspMI       !NNNNNNNNGCAGGT          1   3451
BspMII      T!CCGGA                  0
BsrFI       R!CCGGY                  7   1052   1330   1930   2710   3166
                                         3326   4474
BsrI        NC!CAGT                  9   2316   2471   2593   3077   4034
                                         4440   4558   4601   5040
BsrI        ACTGGN!                  5    349   3286   3909   3922   4870
BssHII      G!CGCGC                  0
Bst1107I    GTA!TAC                  0
BstBI       TT!CGAA                  1   2660
BstEII      G!GTNACC                 0
BstI        G!GATCC                  1   2805
BstNI       CC!WGG                   5    385   3457   3529   3650   3663
BstPI       G!GTNACC                 0
BstXI       CCANNNNN!NTGG            2   2600   2626
BstYI       R!GATCY                  8    133   2805   4142   4153   4239
                                         4251   5019   5036
Bsu36I      CC!TNAGG                 0
CbiI        TT!CGAA                  1   2660
CcrI        C!TCGAG                  1   2637
CelII       GC!TNAGC                 1   2440
Cfr10I      R!CCGGY                  7   1052   1330   1930   2710   3166
                                         3326   4474
Cfr13I      G!GNCC                  13    154    430   2258   2468   2649
                                         2650   3196   3284   4436   4515
                                         4532   4754   5370
Cfr9I       C!CCGGG                  1    426
CfrI        Y!GGCCR                  6    179    346   1372   2631   3336
                                         4782
ClaI        AT!CGAT                  1   1709
CpoI        CG!GWCCG                 0
Csp45I      TT!CGAA                  1   2660
CspI        CG!GSCCG                 0
CvnI        CC!TNAGG                 0
DdeI        C!TNAG                  12    503   1628   1700   1803   2440
                                         2771   2778   3776   4185   4351
                                         4891   5317
DraI        TTT!AAA                  6   1170   2740   3001   4260   4279
                                         4971
DraII       RG!GNCCY                 3   2258   2649   5370
DraIII      CACNNN!GTG               0
DrdI        GACNNNN!NNGTC            1   3609
DsaI        C!CRYGG                  2   2262   2653
DsaV        !CCNGG                  11    383    425    426   2569   3455
                                         3527   3648   3661   3879   4575
                                         4926
EaeI        Y!GGCCR                  6    179    346   1372   2631   3336
                                         4782
EagI        C!GGCCG                  2   2631   3336
Eam1105I    GACNNN!NNGTC             1   4394
EarI        !NNNNGAAGAG              0
EarI        CTCTTCN!                 4    488   1669   2281   5189
Ecl136II    GAG!CTC                  1   2782
EclXI       C!GGCCG                  2   2631   3336
Eco105I     TAC!GTA                  0
Eco31I      GGTCTCN!                 1   2698
Eco31I      !NNNNNGAGACC             2   2559   4455
Eco47I      G!GWCC                   6   2258   2468   3196   3284   4532
                                         4754
Eco47III    AGC!GCT                  2   2438   3174
Eco52I      C!GGCCG                  2   2631   3336
Eco81I      CC!TNAGG                 0
EcoNI       CCTNN!NNNAGG             0
EcoO109I    RG!GNCCY                 3   2258   2649   5370
EcoO65I     G!GTNACC                 0
EcoRI       G!AATTC                  1   2604
EcoRII      !CCWGG                   5    383   3455   3527   3648   3661
EcoRV       GAT!ATC                  1   2616
EcoT14I     C!CWWGG                  1   2262
EcoT22I     ATGCA!T                  1   1407
EcoT38I     GRGCY!C                  2   2653   2784
EheI        GGC!GCC                  1   1391
Esp3I       CGTCTCN!                 2   1662   1674
Esp3I       !NNNNNGAGACG             1   1540
EspI        GC!TNAGC                 1   2440
FbaI        T!GATCA                  4   1821   2749   2788   3042
FokI        GGATGNNNNNNNNN!          2   3397   3442
FokI        !NNNNNNNNNNNNNCATCC      3   4360   4541   4828
FspI        TGC!GCA                  2   1681   4616
GdiII       Y!GGCCG                  4   1372   2631   3336   4782
GdiII       C!GGCCR                  2   2631   3336
HaeI        WGG!CCW                  8    181    348   2776   3317   3389
                                         3446   3527   3979
HaeII       RGCGC!Y                  5   1091   1393   2440   3176   3749
HgaI        GACGCNNNNN!              4   3350   3382   3612   4190
HgaI        !NNNNNNNNNNGCGTC         5    248    743   1128   1644   4920
HgiAI       GWGCW!C                  4   2784   3819   4980   5065
HgiEII      !ACCNNNNNNGGT            1   4081
Hin1I       GR!CGYC                  7    521    983   1390   1566   1655
                                         4931   5313
HincII      GTY!RAC                  5    633   1185   2940   3050   4935
HindIII     A!AGCTT                  0
HinfI       G!ANTC                  10     76    444   1508   2236   2640
                                         2698   3249   3403   3872   4389
HpaI        GTT!AAC                  1   2940
HphI        GGTGANNNNNNNN!           2   4880   5121
HphI        !NNNNNNNTCACC            7    410    978   2713   2719   4237
                                         4464   5086
KasI        G!GCGCC                  1   1389
KpnI        GGTAC!C                  1   2584
Ksp632I     CTCTTCN!                 4    488   1669   2281   5189
Ksp632I     !NNNNGAAGAG              0
KspI        CCGC!GG                  1   2656
MaeI        C!TAG                   10    309   1085   1960   1975   2588
                                         2644   2852   3996   4249   4584
MaeII       A!CGT                   12    171    521    983    992   1566
                                         2037   3298   3354   4204   4620
                                         4993   5313
MamI        GATNN!NNATC              2   1770   3041
MboII       GAAGANNNNNNNN!           5    507    550   1593   1990   4163
MboII       !NNNNNNNTCTTC           11    254    475   1656   2268   3127
                                         3398   4234   4989   5067   5176
                                         5372
McrI        !CGRYCG                  9    388   1327   2630   2797   3049
                                         3335   3837   4760   4909
MfeI        !CAATTG                  2   1417   2948
MflI        R!GATCY                  8    133   2805   4142   4153   4239
                                         4251   5019   5036
MluI        A!CGCGT                  1   2704
MnlI        CCTCNNNNNNN!            12   1294   1585   2529   2693   2698
                                         2766   2805   3025   3272   3683
                                         4561   4767
MroI        T!CCGGA                  0
MscI        TGG!CCA                  2    181    348
MstI        TGC!GCA                  2   1681   4616
MstII       CC!TNAGG                 0
MunI        C!AATTG                  2   1418   2949
MvaI        CC!WGG                   5    385   3457   3529   3650   3663
NaeI        GCC!GGC                  2   3168   3328
NarI        GG!CGCC                  1   1390
NciI        CC!SGG                   6    427    428   2571   3881   4577
                                         4928
NcoI        C!CATGG                  1   2262
NdeI        CA!TATG                  1   2121
NgoMI       G!CCGGC                  2   3166   3326
NheI        G!CTAGC                  0
NotI        GC!GGCCGC                1   2631
NruI        TCG!CGA                  2    515   3371
NsiI        ATGCA!T                  1   1407
Nsp(7524)I  RCATG!Y                  7    600    779   1106   1122   1640
                                         2117   2245
Nsp(7524)V  TT!CGAA                  1   2660
NspBII      CMG!CKG                  8    934   1907   2203   2655   2747
                                         3843   4088   5029
NspII       GDGCH!C                  5   2653   2784   3819   4980   5065
NspIII      C!YCGRG                  2    426   2637
NspIV       G!GNCC                  13    154    430   2258   2468   2649
                                         2650   3196   3284   4436   4515
                                         4532   4754   5370
NunII       GG!CGCC                  1   1390
PacI        TTAAT!TAA                0
PaeR71      C!TCGAG                  1   2637
PflMI       CCANNNN!NTGG             1    384
PleI        GAGTCNNNN!               2   2648   3880
PleI        !NNNNNGACTC              2    438   4383
PmaCI       CAC!GTG                  0
PmeI        GTTT!AAAC                1   2740
PmlI        CAC!GTG                  0
PpuMI       RG!GWCCY                 1   2258
PshAI       GACNN!NNGTC              1   3113
PstI        CTGCA!G                  2   2613   4639
PvuI        CGAT!CG                  3    392   2801   4764
PvuII       CAG!CTG                  2   1907   2203
RmaI        C!TAG                   10    309   1085   1960   1975   2588
                                         2644   2852   3996   4249   4584
RsaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2582   2709   4874
RsrI        G!AATTC                  1   2604
RsrII       CG!GWCCG                 0
SacI        GAGCT!C                  1   2784
SacII       CCGC!GG                  1   2656
SalI        G!TCGAC                  1   3048
Sau96I      G!GNCC                  13    154    430   2258   2468   2649
                                         2650   3196   3284   4436   4515
                                         4532   4754   5370
SauI        CC!TNAGG                 0
ScaI        AGT!ACT                  1   4874
ScrFI       CC!NGG                  11    385    427    428   2571   3457
                                         3529   3650   3663   3881   4577
                                         4928
SduI        GDGCH!C                  5   2653   2784   3819   4980   5065
SecI        C!CNNGG                  7    426    427   2262   2569   2653
                                         2688   3661
SexI        !CTCGAG                  1   2636
SfaNI       GCATCNNNNN!              6     74   1867   3432   3598   4650
                                         5090
SfaNI       !NNNNNNNNNGATGC          8     82    719   1799   1955   2883
                                         3045   3420   4841
SfcI        C!TRYAG                  5    605   2609   3766   3957   4635
SfiI        GGCCNNNN!NGGCC           0
SfuI        TT!CGAA                  1   2660
SgrAI       CR!CCGGYG                0
SinI        G!GWCC                   6   2258   2468   3196   3284   4532
                                         4754
SmaI        CCC!GGG                  1    428
SnaBI       TAC!GTA                  0
SnaI        !GTATAC                  0
SnoI        G!TGCAC                  2   3815   5061
SpeI        A!CTAGT                  2   1959   2587
SphI        GCATG!C                  1   1106
SplI        C!GTACG                  1   1097
SrfI        GCCC!GGGC                0
Sse8387I    CCTGCA!GG                0
SspI        AAT!ATT                  4   1434   1735   2054   5198
SstI        GAGCT!C                  1   2784
SstII       CCGC!GG                  1   2656
SstIII      !ACGT                   12    170    520    982    991   1565
                                         2036   3297   3353   4203   4619
                                         4992   5312
StuI        AGG!CCT                  1   2776
StyI        C!CWWGG                  1   2262
StySJI      !GAGNNNNNNGTRC           0
StySJI      !GYACNNNNNNCTC           2     94   1885
SwaI        ATTT!AAAT                0
TaqI        T!CGA                   14    236    493   1152   1709   2212
                                         2638   2660   2696   2758   2797
                                         2801   3049   3601   5045
TfiI        G!AWTC                   6     76   1508   2236   2698   3249
                                         3403
Tsp45I      !GTSAC                   8    116    467    984   1567   2175
                                         3277   4650   4861
Tth111I     GACN!NNGTC               0
Tth111II    CAARCANNNNNNNNNNN!       5    222   1610   2319   4091   4130
Tth111II    !NNNNNNNNNTGYTTG         5   1288   1439   1631   1665   4098
TthHB8I     T!CGA                   14    236    493   1152   1709   2212
                                         2638   2660   2696   2758   2797
                                         2801   3049   3601   5045
Van91I      CCANNNN!NTGG             1    384
VspI        AT!TAAT                  2     12   4566
XbaI        T!CTAGA                  1   2643
XcmI        CCANNNNN!NNNNTGG         0
XcyI        C!CCGGG                  1    426
XhoI        C!TCGAG                  1   2637
XhoII       R!GATCY                  8    133   2805   4142   4153   4239
                                         4251   5019   5036
XmaI        C!CCGGG                  1    426
XmaIII      C!GGCCG                  2   2631   3336
XmnI        GAANN!NNTTC              4     13    802   1873   4993
XorII       CGAT!CG                  3    392   2801   4764