File: pAc5.1/V5-His C 24-0573
Mode: Normal              1 -    5371
Table: DNASIS#  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatI        AGG!CCT                  1   2768
AatII       GACGT!C                  4    524    986   1569   5308
Acc65I      G!GTACC                  1   2580
AccI        GT!MKAC                  2   1210   3041
AccIII      T!CCGGA                  0
AcyI        GR!CGYC                  7    521    983   1390   1566   1655
                                         4923   5305
AfaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2582   2701   4866
AflI        G!GWCC                   6   2258   2468   3188   3276   4524
                                         4746
AflII       C!TTAAG                  1   1935
AflIII      A!CRYGT                  4    169    596   2113   2696
AgeI        A!CCGGT                  1   2702
AhaI        CC!SGG                   6    427    428   2571   3873   4569
                                         4920
AhaII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4923   5305
AhaIII      TTT!AAA                  6   1170   2732   2993   4252   4271
                                         4963
Alw44I      G!TGCAC                  2   3807   5053
AlwI        GGATCNNNN!               6   2581   2805   3494   4142   4239
                                         5019
AlwI        !NNNNNGATCC              6   2792   4054   4140   4238   4702
                                         5023
AlwNI       CAGNNN!CTG               2   1577   3909
AocI        CC!TNAGG                 0
AocII       GDGCH!C                  4   2776   3811   4972   5057
Aor51HI     AGC!GCT                  2   2438   3166
AosI        TGC!GCA                  2   1681   4608
AosII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4923   5305
ApaI        GGGCC!C                  0
ApaLI       G!TGCAC                  2   3807   5053
ApoI        R!AATTY                  5    508    581   2329   2604   2877
ApyI        CC!WGG                   5    385   3449   3521   3642   3655
AquI        C!YCGRG                  2    426   2637
AscI        GG!CGCGCC                0
AseI        AT!TAAT                  2     12   4558
AsnI        AT!TAAT                  2     12   4558
Asp700I     GAANN!NNTTC              4     13    802   1873   4985
Asp718I     G!GTACC                  1   2580
AspHI       GWGCW!C                  4   2776   3811   4972   5057
AspI        GACN!NNGTC               0
AsuI        G!GNCC                  11    154    430   2258   2468   3188
                                         3276   4428   4507   4524   4746
                                         5362
AsuII       TT!CGAA                  1   2652
AvaI        C!YCGRG                  2    426   2637
AvaII       G!GWCC                   6   2258   2468   3188   3276   4524
                                         4746
AvaIII      ATGCA!T                  1   1407
AviII       TGC!GCA                  2   1681   4608
AvrI        !CYCGRG                  2    425   2636
AvrII       C!CTAGG                  0
AxyI        CC!TNAGG                 0
BalI        TGG!CCA                  2    181    348
BamHI       G!GATCC                  1   2797
BanI        G!GYRCC                  4   1389   2580   3155   4334
BanII       GRGCY!C                  1   2776
BanIII      AT!CGAT                  1   1709
BbeI        GGCGC!C                  1   1393
BbiII/AcyI  GR!CGYC                  7    521    983   1390   1566   1655
                                         4923   5305
BbrPI       CAC!GTG                  0
BbsI        !NNNNNNGTCTTC            3    254   3119   5364
BbsI        GAAGACNN!                0
BbvI        GCAGCNNNNNNNN!           9    943   1254   1636   1916   3174
                                         3912   3915   4121   4815
BbvI        !NNNNNNNNNNNNGCTGC       7    127   1828   2449   2903   3822
                                         4424   4613
BbvII       GAAGACNN!                0
BbvII       !NNNNNNGTCTTC            3    254   3119   5364
BcefI       ACGGCNNNNNNNNNNNN!       3   1338   1871   3995
BcefI       !NNNNNNNNNNNNNGCCGT      2   1111   1901
BclI        T!GATCA                  4   1821   2741   2780   3034
BcnI        CC!SGG                   6    427    428   2571   3873   4569
                                         4920
BfaI        C!TAG                    9    309   1085   1960   1975   2588
                                         2844   3988   4241   4576
BfrI        C!TTAAG                  1   1935
BglI        GCCNNNN!NGGC             2   3324   4506
BglII       A!GATCT                  1    133
BinI        !NNNNNGATCC              6   2792   4054   4140   4238   4702
                                         5023
BinI        GGATCNNNN!               6   2581   2805   3494   4142   4239
                                         5019
BlnI        C!CTAGG                  0
BmyI        GDGCH!C                  4   2776   3811   4972   5057
Bpu1102I    GC!TNAGC                 1   2440
BsaAI       YAC!GTR                  0
BsaBI       GATNN!NNATC              2   1770   3033
BsaHI       GR!CGYC                  7    521    983   1390   1566   1655
                                         4923   5305
BsaI        GGTCTCN!                 1   2690
BsaI        !NNNNNGAGACC             2   2559   4447
BsaJI       C!CNNGG                  6    426    427   2262   2569   2680
                                         3653
BseAI       T!CCGGA                  0
BsePI       !GCGCGC                  0
BsiEI       CGRY!CG                  9    392   1331   2634   2793   3045
                                         3331   3833   4756   4905
BsiWI       C!GTACG                  1   1097
BsiYI       CCNNNNN!NNGG            15    384    427   1052   2321   2377
                                         2575   2576   2577   2654   2681
                                         3085   3515   3533   3699   3978
BslI        CCNNNNN!NNGG            15    384    427   1052   2321   2377
                                         2575   2576   2577   2654   2681
                                         3085   3515   3533   3699   3978
BsmAI       !NNNNNGAGAC              3   1540   2559   4447
BsmAI       GTCTCN!                  6    483   1045   1662   1674   2690
                                         5223
BsmI        NG!CATTC                 1   2946
BsmI        GAATGCN!                 4    285    504   1407   2853
Bsp1286I    GDGCH!C                  4   2776   3811   4972   5057
BspDI       AT!CGAT                  1   1709
BspEI       T!CCGGA                  0
BspHI       T!CATGA                  4     56   4213   5221   5326
BspMI       ACCTGCNNNN!              0
BspMI       !NNNNNNNNGCAGGT          1   3443
BspMII      T!CCGGA                  0
BsrFI       R!CCGGY                  7   1052   1330   1930   2702   3158
                                         3318   4466
BsrI        NC!CAGT                  9   2316   2471   2593   3069   4026
                                         4432   4550   4593   5032
BsrI        ACTGGN!                  5    349   3278   3901   3914   4862
BssHII      G!CGCGC                  0
Bst1107I    GTA!TAC                  0
BstBI       TT!CGAA                  1   2652
BstEII      G!GTNACC                 1   2642
BstI        G!GATCC                  1   2797
BstNI       CC!WGG                   5    385   3449   3521   3642   3655
BstPI       G!GTNACC                 1   2642
BstXI       CCANNNNN!NTGG            2   2600   2626
BstYI       R!GATCY                  8    133   2797   4134   4145   4231
                                         4243   5011   5028
Bsu36I      CC!TNAGG                 0
CbiI        TT!CGAA                  1   2652
CcrI        C!TCGAG                  1   2637
CelII       GC!TNAGC                 1   2440
Cfr10I      R!CCGGY                  7   1052   1330   1930   2702   3158
                                         3318   4466
Cfr13I      G!GNCC                  11    154    430   2258   2468   3188
                                         3276   4428   4507   4524   4746
                                         5362
Cfr9I       C!CCGGG                  1    426
CfrI        Y!GGCCR                  6    179    346   1372   2631   3328
                                         4774
ClaI        AT!CGAT                  1   1709
CpoI        CG!GWCCG                 0
Csp45I      TT!CGAA                  1   2652
CspI        CG!GSCCG                 0
CvnI        CC!TNAGG                 0
DdeI        C!TNAG                  12    503   1628   1700   1803   2440
                                         2763   2770   3768   4177   4343
                                         4883   5309
DraI        TTT!AAA                  6   1170   2732   2993   4252   4271
                                         4963
DraII       RG!GNCCY                 2   2258   5362
DraIII      CACNNN!GTG               0
DrdI        GACNNNN!NNGTC            1   3601
DsaI        C!CRYGG                  1   2262
DsaV        !CCNGG                  11    383    425    426   2569   3447
                                         3519   3640   3653   3871   4567
                                         4918
EaeI        Y!GGCCR                  6    179    346   1372   2631   3328
                                         4774
EagI        C!GGCCG                  2   2631   3328
Eam1105I    GACNNN!NNGTC             1   4386
EarI        !NNNNGAAGAG              0
EarI        CTCTTCN!                 4    488   1669   2281   5181
Ecl136II    GAG!CTC                  1   2774
EclXI       C!GGCCG                  2   2631   3328
Eco105I     TAC!GTA                  0
Eco31I      GGTCTCN!                 1   2690
Eco31I      !NNNNNGAGACC             2   2559   4447
Eco47I      G!GWCC                   6   2258   2468   3188   3276   4524
                                         4746
Eco47III    AGC!GCT                  2   2438   3166
Eco52I      C!GGCCG                  2   2631   3328
Eco81I      CC!TNAGG                 0
EcoNI       CCTNN!NNNAGG             0
EcoO109I    RG!GNCCY                 2   2258   5362
EcoO65I     G!GTNACC                 1   2642
EcoRI       G!AATTC                  1   2604
EcoRII      !CCWGG                   5    383   3447   3519   3640   3653
EcoRV       GAT!ATC                  1   2616
EcoT14I     C!CWWGG                  1   2262
EcoT22I     ATGCA!T                  1   1407
EcoT38I     GRGCY!C                  1   2776
EheI        GGC!GCC                  1   1391
Esp3I       CGTCTCN!                 2   1662   1674
Esp3I       !NNNNNGAGACG             1   1540
EspI        GC!TNAGC                 1   2440
FbaI        T!GATCA                  4   1821   2741   2780   3034
FokI        GGATGNNNNNNNNN!          2   3389   3434
FokI        !NNNNNNNNNNNNNCATCC      3   4352   4533   4820
FspI        TGC!GCA                  2   1681   4608
GdiII       Y!GGCCG                  4   1372   2631   3328   4774
GdiII       C!GGCCR                  2   2631   3328
HaeI        WGG!CCW                  8    181    348   2768   3309   3381
                                         3438   3519   3971
HaeII       RGCGC!Y                  5   1091   1393   2440   3168   3741
HgaI        GACGCNNNNN!              4   3342   3374   3604   4182
HgaI        !NNNNNNNNNNGCGTC         5    248    743   1128   1644   4912
HgiAI       GWGCW!C                  4   2776   3811   4972   5057
HgiEII      !ACCNNNNNNGGT            1   4073
Hin1I       GR!CGYC                  7    521    983   1390   1566   1655
                                         4923   5305
HincII      GTY!RAC                  5    633   1185   2932   3042   4927
HindIII     A!AGCTT                  0
HinfI       G!ANTC                   9     76    444   1508   2236   2690
                                         3241   3395   3864   4381
HpaI        GTT!AAC                  1   2932
HphI        GGTGANNNNNNNN!           2   4872   5113
HphI        !NNNNNNNTCACC            8    410    978   2636   2705   2711
                                         4229   4456   5078
KasI        G!GCGCC                  1   1389
KpnI        GGTAC!C                  1   2584
Ksp632I     CTCTTCN!                 4    488   1669   2281   5181
Ksp632I     !NNNNGAAGAG              0
KspI        CCGC!GG                  0
MaeI        C!TAG                    9    309   1085   1960   1975   2588
                                         2844   3988   4241   4576
MaeII       A!CGT                   12    171    521    983    992   1566
                                         2037   3290   3346   4196   4612
                                         4985   5305
MamI        GATNN!NNATC              2   1770   3033
MboII       GAAGANNNNNNNN!           5    507    550   1593   1990   4155
MboII       !NNNNNNNTCTTC           11    254    475   1656   2268   3119
                                         3390   4226   4981   5059   5168
                                         5364
McrI        !CGRYCG                  9    388   1327   2630   2789   3041
                                         3327   3829   4752   4901
MfeI        !CAATTG                  2   1417   2940
MflI        R!GATCY                  8    133   2797   4134   4145   4231
                                         4243   5011   5028
MluI        A!CGCGT                  1   2696
MnlI        CCTCNNNNNNN!            12   1294   1585   2529   2685   2690
                                         2758   2797   3017   3264   3675
                                         4553   4759
MroI        T!CCGGA                  0
MscI        TGG!CCA                  2    181    348
MstI        TGC!GCA                  2   1681   4608
MstII       CC!TNAGG                 0
MunI        C!AATTG                  2   1418   2941
MvaI        CC!WGG                   5    385   3449   3521   3642   3655
NaeI        GCC!GGC                  2   3160   3320
NarI        GG!CGCC                  1   1390
NciI        CC!SGG                   6    427    428   2571   3873   4569
                                         4920
NcoI        C!CATGG                  1   2262
NdeI        CA!TATG                  1   2121
NgoMI       G!CCGGC                  2   3158   3318
NheI        G!CTAGC                  0
NotI        GC!GGCCGC                1   2631
NruI        TCG!CGA                  2    515   3363
NsiI        ATGCA!T                  1   1407
Nsp(7524)I  RCATG!Y                  7    600    779   1106   1122   1640
                                         2117   2245
Nsp(7524)V  TT!CGAA                  1   2652
NspBII      CMG!CKG                  7    934   1907   2203   2739   3835
                                         4080   5021
NspII       GDGCH!C                  4   2776   3811   4972   5057
NspIII      C!YCGRG                  2    426   2637
NspIV       G!GNCC                  11    154    430   2258   2468   3188
                                         3276   4428   4507   4524   4746
                                         5362
NunII       GG!CGCC                  1   1390
PacI        TTAAT!TAA                0
PaeR71      C!TCGAG                  1   2637
PflMI       CCANNNN!NTGG             1    384
PleI        GAGTCNNNN!               1   3872
PleI        !NNNNNGACTC              2    438   4375
PmaCI       CAC!GTG                  0
PmeI        GTTT!AAAC                1   2732
PmlI        CAC!GTG                  0
PpuMI       RG!GWCCY                 1   2258
PshAI       GACNN!NNGTC              1   3105
PstI        CTGCA!G                  2   2613   4631
PvuI        CGAT!CG                  3    392   2793   4756
PvuII       CAG!CTG                  2   1907   2203
RmaI        C!TAG                    9    309   1085   1960   1975   2588
                                         2844   3988   4241   4576
RsaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2582   2701   4866
RsrI        G!AATTC                  1   2604
RsrII       CG!GWCCG                 0
SacI        GAGCT!C                  1   2776
SacII       CCGC!GG                  0
SalI        G!TCGAC                  1   3040
Sau96I      G!GNCC                  11    154    430   2258   2468   3188
                                         3276   4428   4507   4524   4746
                                         5362
SauI        CC!TNAGG                 0
ScaI        AGT!ACT                  1   4866
ScrFI       CC!NGG                  11    385    427    428   2571   3449
                                         3521   3642   3655   3873   4569
                                         4920
SduI        GDGCH!C                  4   2776   3811   4972   5057
SecI        C!CNNGG                  6    426    427   2262   2569   2680
                                         3653
SexI        !CTCGAG                  1   2636
SfaNI       GCATCNNNNN!              6     74   1867   3424   3590   4642
                                         5082
SfaNI       !NNNNNNNNNGATGC          8     82    719   1799   1955   2875
                                         3037   3412   4833
SfcI        C!TRYAG                  5    605   2609   3758   3949   4627
SfiI        GGCCNNNN!NGGCC           0
SfuI        TT!CGAA                  1   2652
SgrAI       CR!CCGGYG                0
SinI        G!GWCC                   6   2258   2468   3188   3276   4524
                                         4746
SmaI        CCC!GGG                  1    428
SnaBI       TAC!GTA                  0
SnaI        !GTATAC                  0
SnoI        G!TGCAC                  2   3807   5053
SpeI        A!CTAGT                  2   1959   2587
SphI        GCATG!C                  1   1106
SplI        C!GTACG                  1   1097
SrfI        GCCC!GGGC                0
Sse8387I    CCTGCA!GG                0
SspI        AAT!ATT                  4   1434   1735   2054   5190
SstI        GAGCT!C                  1   2776
SstII       CCGC!GG                  0
SstIII      !ACGT                   12    170    520    982    991   1565
                                         2036   3289   3345   4195   4611
                                         4984   5304
StuI        AGG!CCT                  1   2768
StyI        C!CWWGG                  1   2262
StySJI      !GAGNNNNNNGTRC           0
StySJI      !GYACNNNNNNCTC           2     94   1885
SwaI        ATTT!AAAT                0
TaqI        T!CGA                   14    236    493   1152   1709   2212
                                         2638   2652   2688   2750   2789
                                         2793   3041   3593   5037
TfiI        G!AWTC                   6     76   1508   2236   2690   3241
                                         3395
Tsp45I      !GTSAC                   9    116    467    984   1567   2175
                                         2642   3269   4642   4853
Tth111I     GACN!NNGTC               0
Tth111II    CAARCANNNNNNNNNNN!       5    222   1610   2319   4083   4122
Tth111II    !NNNNNNNNNTGYTTG         5   1288   1439   1631   1665   4090
TthHB8I     T!CGA                   14    236    493   1152   1709   2212
                                         2638   2652   2688   2750   2789
                                         2793   3041   3593   5037
Van91I      CCANNNN!NTGG             1    384
VspI        AT!TAAT                  2     12   4558
XbaI        T!CTAGA                  0
XcmI        CCANNNNN!NNNNTGG         0
XcyI        C!CCGGG                  1    426
XhoI        C!TCGAG                  1   2637
XhoII       R!GATCY                  8    133   2797   4134   4145   4231
                                         4243   5011   5028
XmaI        C!CCGGG                  1    426
XmaIII      C!GGCCG                  2   2631   3328
XmnI        GAANN!NNTTC              4     13    802   1873   4985
XorII       CGAT!CG                  3    392   2793   4756