File: pAc5/V5-His B 24-0483
Mode: Normal              1 -    5383
Table: DNASIS#  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatI        AGG!CCT                  1   2780
AatII       GACGT!C                  4    524    986   1569   5320
Acc65I      G!GTACC                  1   2584
AccI        GT!MKAC                  2   1210   3053
AccIII      T!CCGGA                  0
AcyI        GR!CGYC                  7    521    983   1390   1566   1655
                                         4935   5317
AfaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2586   2713   4878
AflI        G!GWCC                   6   2258   2468   3200   3288   4536
                                         4758
AflII       C!TTAAG                  1   1935
AflIII      A!CRYGT                  4    169    596   2113   2708
AgeI        A!CCGGT                  1   2714
AhaI        CC!SGG                   6    427    428   2571   3885   4581
                                         4932
AhaII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4935   5317
AhaIII      TTT!AAA                  6   1170   2744   3005   4264   4283
                                         4975
Alw44I      G!TGCAC                  2   3819   5065
AlwI        GGATCNNNN!               6   2581   2817   3506   4154   4251
                                         5031
AlwI        !NNNNNGATCC              7   2568   2804   4066   4152   4250
                                         4714   5035
AlwNI       CAGNNN!CTG               2   1577   3921
AocI        CC!TNAGG                 0
AocII       GDGCH!C                  5   2657   2788   3823   4984   5069
Aor51HI     AGC!GCT                  2   2438   3178
AosI        TGC!GCA                  2   1681   4620
AosII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4935   5317
ApaI        GGGCC!C                  1   2657
ApaLI       G!TGCAC                  2   3819   5065
ApoI        R!AATTY                  5    508    581   2329   2608   2889
ApyI        CC!WGG                   5    385   3461   3533   3654   3667
AquI        C!YCGRG                  2    426   2641
AscI        GG!CGCGCC                0
AseI        AT!TAAT                  2     12   4570
AsnI        AT!TAAT                  2     12   4570
Asp700I     GAANN!NNTTC              4     13    802   1873   4997
Asp718I     G!GTACC                  1   2584
AspHI       GWGCW!C                  4   2788   3823   4984   5069
AspI        GACN!NNGTC               0
AsuI        G!GNCC                  13    154    430   2258   2468   2653
                                         2654   3200   3288   4440   4519
                                         4536   4758   5374
AsuII       TT!CGAA                  1   2664
AvaI        C!YCGRG                  2    426   2641
AvaII       G!GWCC                   6   2258   2468   3200   3288   4536
                                         4758
AvaIII      ATGCA!T                  1   1407
AviII       TGC!GCA                  2   1681   4620
AvrI        !CYCGRG                  2    425   2640
AvrII       C!CTAGG                  0
AxyI        CC!TNAGG                 0
BalI        TGG!CCA                  2    181    348
BamHI       G!GATCC                  2   2573   2809
BanI        G!GYRCC                  4   1389   2584   3167   4346
BanII       GRGCY!C                  2   2657   2788
BanIII      AT!CGAT                  1   1709
BbeI        GGCGC!C                  1   1393
BbiII/AcyI  GR!CGYC                  7    521    983   1390   1566   1655
                                         4935   5317
BbrPI       CAC!GTG                  0
BbsI        !GTCTTC                  3    260   3137   5382
BbsI        !GAAGAC                  0
BbvI        GCAGCNNNNNNNN!           9    943   1254   1636   1916   3186
                                         3924   3927   4133   4827
BbvI        !NNNNNNNNNNNNGCTGC       7    127   1828   2449   2915   3834
                                         4436   4625
BbvII       GAAGACNN!                0
BbvII       !NNNNNNGTCTTC            3    254   3131   5376
BcefI       ACGGCNNNNNNNNNNNN!       3   1338   1871   4007
BcefI       !NNNNNNNNNNNNNGCCGT      2   1111   1901
BcgI        !GCANNNNNNTCG            2   1903   3108
BcgI        !CGANNNNNNTGC            2    764   4913
BclI        T!GATCA                  4   1821   2753   2792   3046
BcnI        CC!SGG                   6    427    428   2571   3885   4581
                                         4932
BfaI        C!TAG                   10    309   1085   1960   1975   2592
                                         2648   2856   4000   4253   4588
BfrI        C!TTAAG                  1   1935
BglI        GCCNNNN!NGGC             2   3336   4518
BglII       A!GATCT                  1    133
BinI        !NNNNNGATCC              7   2568   2804   4066   4152   4250
                                         4714   5035
BinI        GGATCNNNN!               6   2581   2817   3506   4154   4251
                                         5031
BlnI        C!CTAGG                  0
BmyI        GDGCH!C                  5   2657   2788   3823   4984   5069
BpmI        !CTCCAG                  1   4482
BpmI        !CTGGAG                  1   3211
Bpu1102I    GC!TNAGC                 1   2440
BsaAI       YAC!GTR                  0
BsaBI       GATNN!NNATC              2   1770   3045
BsaHI       GR!CGYC                  7    521    983   1390   1566   1655
                                         4935   5317
BsaI        GGTCTCN!                 1   2702
BsaI        !NNNNNGAGACC             2   2559   4459
BsaJI       C!CNNGG                  7    426    427   2262   2569   2657
                                         2692   3665
BseAI       T!CCGGA                  0
BsePI       !GCGCGC                  0
BsgI        !GTGCAG                  2   1239   2020
BsgI        !CTGCAC                  1   1694
BsiEI       CGRY!CG                  9    392   1331   2638   2805   3057
                                         3343   3845   4768   4917
BsiWI       C!GTACG                  1   1097
BsiYI       CCNNNNN!NNGG            11    384    427   1052   2321   2377
                                         2693   3097   3527   3545   3711
                                         3990
BslI        CCNNNNN!NNGG            11    384    427   1052   2321   2377
                                         2693   3097   3527   3545   3711
                                         3990
BsmAI       !GTCTC                   6    477   1039   1656   1668   2696
                                         5229
BsmAI       !GAGAC                   3   1545   2564   4464
BsmI        GAATGCN!                 4    285    504   1407   2865
BsmI        G!CATTC                  1   2958
Bsp1286I    GDGCH!C                  5   2657   2788   3823   4984   5069
BspDI       AT!CGAT                  1   1709
BspEI       T!CCGGA                  0
BspHI       T!CATGA                  4     56   4225   5233   5338
BspMI       ACCTGCNNNN!              0
BspMI       !NNNNNNNNGCAGGT          1   3455
BspMII      T!CCGGA                  0
BsrFI       R!CCGGY                  7   1052   1330   1930   2714   3170
                                         3330   4478
BsrI        ACTGGN!                  5    349   3290   3913   3926   4874
BsrI        C!CAGT                   9   2316   2471   2597   3081   4038
                                         4444   4562   4605   5044
BssHII      G!CGCGC                  0
Bst1107I    GTA!TAC                  0
BstBI       TT!CGAA                  1   2664
BstEII      G!GTNACC                 0
BstI        G!GATCC                  2   2573   2809
BstNI       CC!WGG                   5    385   3461   3533   3654   3667
BstPI       G!GTNACC                 0
BstXI       CCANNNNN!NTGG            2   2604   2630
BstYI       R!GATCY                  9    133   2573   2809   4146   4157
                                         4243   4255   5023   5040
Bsu36I      CC!TNAGG                 0
CbiI        TT!CGAA                  1   2664
CcrI        C!TCGAG                  1   2641
CelII       GC!TNAGC                 1   2440
Cfr10I      R!CCGGY                  7   1052   1330   1930   2714   3170
                                         3330   4478
Cfr13I      G!GNCC                  13    154    430   2258   2468   2653
                                         2654   3200   3288   4440   4519
                                         4536   4758   5374
Cfr9I       C!CCGGG                  1    426
CfrI        Y!GGCCR                  6    179    346   1372   2635   3340
                                         4786
ClaI        AT!CGAT                  1   1709
CpoI        CG!GWCCG                 0
Csp45I      TT!CGAA                  1   2664
CspI        CG!GSCCG                 0
CvnI        CC!TNAGG                 0
DdeI        C!TNAG                  12    503   1628   1700   1803   2440
                                         2775   2782   3780   4189   4355
                                         4895   5321
DraI        TTT!AAA                  6   1170   2744   3005   4264   4283
                                         4975
DraII       RG!GNCCY                 3   2258   2653   5374
DraIII      CACNNN!GTG               0
DrdI        GACNNNN!NNGTC            1   3613
DsaI        C!CRYGG                  2   2262   2657
DsaV        !CCNGG                  11    383    425    426   2569   3459
                                         3531   3652   3665   3883   4579
                                         4930
EaeI        Y!GGCCR                  6    179    346   1372   2635   3340
                                         4786
EagI        C!GGCCG                  2   2635   3340
Eam1105I    GACNNN!NNGTC             1   4398
EarI        !CTCTTC                  4    481   1662   2274   5186
EarI        !GAAGAG                  0
Ecl136II    GAG!CTC                  1   2786
EclXI       C!GGCCG                  2   2635   3340
Eco105I     TAC!GTA                  0
Eco31I      GGTCTCN!                 1   2702
Eco31I      !NNNNNGAGACC             2   2559   4459
Eco47I      G!GWCC                   6   2258   2468   3200   3288   4536
                                         4758
Eco47III    AGC!GCT                  2   2438   3178
Eco52I      C!GGCCG                  2   2635   3340
Eco57I      !CTGAAG                  2   2203   4031
Eco57I      !CTTCAG                  1   5079
Eco81I      CC!TNAGG                 0
EcoNI       CCTNN!NNNAGG             0
EcoO109I    RG!GNCCY                 3   2258   2653   5374
EcoO65I     G!GTNACC                 0
EcoRI       G!AATTC                  1   2608
EcoRII      !CCWGG                   5    383   3459   3531   3652   3665
EcoRV       GAT!ATC                  1   2620
EcoT14I     C!CWWGG                  1   2262
EcoT22I     ATGCA!T                  1   1407
EcoT38I     GRGCY!C                  2   2657   2788
EheI        GGC!GCC                  1   1391
Esp3I       CGTCTCN!                 2   1662   1674
Esp3I       !NNNNNGAGACG             1   1540
EspI        GC!TNAGC                 1   2440
FbaI        T!GATCA                  4   1821   2753   2792   3046
FinI        !GTCCC                   2   1998   3288
FinI        !GGGAC                   2   2466   3484
FokI        GGATGNNNNNNNNN!          2   3401   3446
FokI        !NNNNNNNNNNNNNCATCC      3   4364   4545   4832
FspI        TGC!GCA                  2   1681   4620
GdiII       !NNNNNYGGCCG             4   1366   2629   3334   4780
GdiII       CGGCCRN!                 2   2641   3346
GsuI        !CTCCAG                  1   4482
GsuI        !CTGGAG                  1   3211
HaeI        WGG!CCW                  8    181    348   2780   3321   3393
                                         3450   3531   3983
HaeII       RGCGC!Y                  5   1091   1393   2440   3180   3753
HgaI        GACGCNNNNN!              4   3354   3386   3616   4194
HgaI        !NNNNNNNNNNGCGTC         5    248    743   1128   1644   4924
HgiAI       GWGCW!C                  4   2788   3823   4984   5069
HgiEII      !ACCNNNNNNGGT            1   4085
Hin1I       GR!CGYC                  7    521    983   1390   1566   1655
                                         4935   5317
HincII      GTY!RAC                  5    633   1185   2944   3054   4939
HindIII     A!AGCTT                  0
HinfI       G!ANTC                  10     76    444   1508   2236   2644
                                         2702   3253   3407   3876   4393
HpaI        GTT!AAC                  1   2944
HphI        GGTGANNNNNNNN!           2   4884   5125
HphI        !NNNNNNNTCACC            7    410    978   2717   2723   4241
                                         4468   5090
KasI        G!GCGCC                  1   1389
KpnI        GGTAC!C                  1   2588
Ksp632I     CTCTTCN!                 4    488   1669   2281   5193
Ksp632I     !NNNNGAAGAG              0
KspI        CCGC!GG                  1   2660
MaeI        C!TAG                   10    309   1085   1960   1975   2592
                                         2648   2856   4000   4253   4588
MaeII       A!CGT                   12    171    521    983    992   1566
                                         2037   3302   3358   4208   4624
                                         4997   5317
MamI        GATNN!NNATC              2   1770   3045
MboII       GAAGANNNNNNNN!           5    507    550   1593   1990   4167
MboII       !NNNNNNNTCTTC           11    254    475   1656   2268   3131
                                         3402   4238   4993   5071   5180
                                         5376
McrI        !CGRYCG                  9    388   1327   2634   2801   3053
                                         3339   3841   4764   4913
MfeI        !CAATTG                  2   1417   2952
MflI        R!GATCY                  9    133   2573   2809   4146   4157
                                         4243   4255   5023   5040
MluI        A!CGCGT                  1   2708
MmeI        !TCCRAC                  2   3694   3878
MmeI        !GTYGGA                  1    993
MnlI        CCTCNNNNNNN!            12   1294   1585   2529   2697   2702
                                         2770   2809   3029   3276   3687
                                         4565   4771
MroI        T!CCGGA                  0
MscI        TGG!CCA                  2    181    348
MstI        TGC!GCA                  2   1681   4620
MstII       CC!TNAGG                 0
MunI        C!AATTG                  2   1418   2953
MvaI        CC!WGG                   5    385   3461   3533   3654   3667
NaeI        GCC!GGC                  2   3172   3332
NarI        GG!CGCC                  1   1390
NciI        CC!SGG                   6    427    428   2571   3885   4581
                                         4932
NcoI        C!CATGG                  1   2262
NdeI        CA!TATG                  1   2121
NgoMI       G!CCGGC                  2   3170   3330
NheI        G!CTAGC                  0
NotI        GC!GGCCGC                1   2635
NruI        TCG!CGA                  2    515   3375
NsiI        ATGCA!T                  1   1407
Nsp(7524)I  RCATG!Y                  7    600    779   1106   1122   1640
                                         2117   2245
Nsp(7524)V  TT!CGAA                  1   2664
NspBII      CMG!CKG                  8    934   1907   2203   2659   2751
                                         3847   4092   5033
NspII       GDGCH!C                  5   2657   2788   3823   4984   5069
NspIII      C!YCGRG                  2    426   2641
NspIV       G!GNCC                  13    154    430   2258   2468   2653
                                         2654   3200   3288   4440   4519
                                         4536   4758   5374
NunII       GG!CGCC                  1   1390
PacI        TTAAT!TAA                0
PaeR71      C!TCGAG                  1   2641
PflMI       CCANNNN!NTGG             1    384
PleI        GAGTCNNNN!               2   2652   3884
PleI        !NNNNNGACTC              2    438   4387
PmaCI       CAC!GTG                  0
PmeI        GTTT!AAAC                1   2744
PmlI        CAC!GTG                  0
PpuMI       RG!GWCCY                 1   2258
PshAI       GACNN!NNGTC              1   3117
PstI        CTGCA!G                  2   2617   4643
PvuI        CGAT!CG                  3    392   2805   4768
PvuII       CAG!CTG                  2   1907   2203
RmaI        C!TAG                   10    309   1085   1960   1975   2592
                                         2648   2856   4000   4253   4588
RsaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2586   2713   4878
RsrI        G!AATTC                  1   2608
RsrII       CG!GWCCG                 0
SacI        GAGCT!C                  1   2788
SacII       CCGC!GG                  1   2660
SalI        G!TCGAC                  1   3052
Sap1        GCTCTTCN!                0
Sau96I      G!GNCC                  13    154    430   2258   2468   2653
                                         2654   3200   3288   4440   4519
                                         4536   4758   5374
SauI        CC!TNAGG                 0
ScaI        AGT!ACT                  1   4878
ScrFI       CC!NGG                  11    385    427    428   2571   3461
                                         3533   3654   3667   3885   4581
                                         4932
SduI        GDGCH!C                  5   2657   2788   3823   4984   5069
SecI        C!CNNGG                  7    426    427   2262   2569   2657
                                         2692   3665
SexI        !CTCGAG                  1   2640
SfaNI       GCATCNNNNN!              6     74   1867   3436   3602   4654
                                         5094
SfaNI       !NNNNNNNNNGATGC          8     82    719   1799   1955   2887
                                         3049   3424   4845
SfcI        C!TYRAG                  9   1935   2400   2641   3065   3271
                                         3611   3873   4150   5018
SfiI        GGCCNNNN!NGGCC           0
SfuI        TT!CGAA                  1   2664
SgrAI       CR!CCGGYG                0
SinI        G!GWCC                   6   2258   2468   3200   3288   4536
                                         4758
SmaI        CCC!GGG                  1    428
SnaBI       TAC!GTA                  0
SnaI        !GTATAC                  0
SnoI        G!TGCAC                  2   3819   5065
SpeI        A!CTAGT                  2   1959   2591
SphI        GCATG!C                  1   1106
SplI        C!GTACG                  1   1097
SrfI        GCCC!GGGC                0
Sse8387I    CCTGCA!GG                0
SspI        AAT!ATT                  4   1434   1735   2054   5202
SstI        GAGCT!C                  1   2788
SstII       CCGC!GG                  1   2660
SstIII      !ACGT                   12    170    520    982    991   1565
                                         2036   3301   3357   4207   4623
                                         4996   5316
StuI        AGG!CCT                  1   2780
StyI        C!CWWGG                  1   2262
StySJI      !GAGNNNNNNGTRC           0
StySJI      !GYACNNNNNNCTC           2     94   1885
SwaI        ATTT!AAAT                0
TaqI        T!CGA                   14    236    493   1152   1709   2212
                                         2642   2664   2700   2762   2801
                                         2805   3053   3605   5049
TaqII       GACCGANNNNNNNNNNN!       2   3071   4931
TaqII       !NNNNNNNNNTCGGTC         2   2684   4746
TaqII       CACCCANNNNNNNNNNN!       2   2329   5084
TaqII       !NNNNNNNNNTGGGTG         1   5101
TfiI        G!AWTC                   6     76   1508   2236   2702   3253
                                         3407
Tsp45I      !GTSAC                   8    116    467    984   1567   2175
                                         3281   4654   4865
Tth111I     GACN!NNGTC               0
Tth111II    CAARCANNNNNNNNNNN!       5    222   1610   2319   4095   4134
Tth111II    !NNNNNNNNNTGYTTG         5   1288   1439   1631   1665   4102
TthHB8I     T!CGA                   14    236    493   1152   1709   2212
                                         2642   2664   2700   2762   2801
                                         2805   3053   3605   5049
Van91I      CCANNNN!NTGG             1    384
VspI        AT!TAAT                  2     12   4570
XbaI        T!CTAGA                  1   2647
XcmI        !CCANNNNNNNNNTCG         2   2199   3294
XcyI        C!CCGGG                  1    426
XhoI        C!TCGAG                  1   2641
XhoII       R!GATCY                  9    133   2573   2809   4146   4157
                                         4243   4255   5023   5040
XmaI        C!CCGGG                  1    426
XmaIII      C!GGCCG                  2   2635   3340
XmnI        GAANN!NNTTC              4     13    802   1873   4997
XorII       CGAT!CG                  3    392   2805   4768