File: pAc5/V5-His C 24-0485
Mode: Normal              1 -    5375
Table: DNASIS#  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatI        AGG!CCT                  1   2772
AatII       GACGT!C                  4    524    986   1569   5312
Acc65I      G!GTACC                  1   2584
AccI        GT!MKAC                  2   1210   3045
AccIII      T!CCGGA                  0
AcyI        GR!CGYC                  7    521    983   1390   1566   1655
                                         4927   5309
AfaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2586   2705   4870
AflI        G!GWCC                   6   2258   2468   3192   3280   4528
                                         4750
AflII       C!TTAAG                  1   1935
AflIII      A!CRYGT                  4    169    596   2113   2700
AgeI        A!CCGGT                  1   2706
AhaI        CC!SGG                   6    427    428   2571   3877   4573
                                         4924
AhaII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4927   5309
AhaIII      TTT!AAA                  6   1170   2736   2997   4256   4275
                                         4967
Alw44I      G!TGCAC                  2   3811   5057
AlwI        GGATCNNNN!               6   2581   2809   3498   4146   4243
                                         5023
AlwI        !NNNNNGATCC              7   2568   2796   4058   4144   4242
                                         4706   5027
AlwNI       CAGNNN!CTG               2   1577   3913
AocI        CC!TNAGG                 0
AocII       GDGCH!C                  4   2780   3815   4976   5061
Aor51HI     AGC!GCT                  2   2438   3170
AosI        TGC!GCA                  2   1681   4612
AosII       GR!CGYC                  7    521    983   1390   1566   1655
                                         4927   5309
ApaI        GGGCC!C                  0
ApaLI       G!TGCAC                  2   3811   5057
ApoI        R!AATTY                  5    508    581   2329   2608   2881
ApyI        CC!WGG                   5    385   3453   3525   3646   3659
AquI        C!YCGRG                  2    426   2641
AscI        GG!CGCGCC                0
AseI        AT!TAAT                  2     12   4562
AsnI        AT!TAAT                  2     12   4562
Asp700I     GAANN!NNTTC              4     13    802   1873   4989
Asp718I     G!GTACC                  1   2584
AspHI       GWGCW!C                  4   2780   3815   4976   5061
AspI        GACN!NNGTC               0
AsuI        G!GNCC                  11    154    430   2258   2468   3192
                                         3280   4432   4511   4528   4750
                                         5366
AsuII       TT!CGAA                  1   2656
AvaI        C!YCGRG                  2    426   2641
AvaII       G!GWCC                   6   2258   2468   3192   3280   4528
                                         4750
AvaIII      ATGCA!T                  1   1407
AviII       TGC!GCA                  2   1681   4612
AvrI        !CYCGRG                  2    425   2640
AvrII       C!CTAGG                  0
AxyI        CC!TNAGG                 0
BalI        TGG!CCA                  2    181    348
BamHI       G!GATCC                  2   2573   2801
BanI        G!GYRCC                  4   1389   2584   3159   4338
BanII       GRGCY!C                  1   2780
BanIII      AT!CGAT                  1   1709
BbeI        GGCGC!C                  1   1393
BbiII/AcyI  GR!CGYC                  7    521    983   1390   1566   1655
                                         4927   5309
BbrPI       CAC!GTG                  0
BbsI        !GTCTTC                  3    260   3129   5374
BbsI        !GAAGAC                  0
BbvI        GCAGCNNNNNNNN!           9    943   1254   1636   1916   3178
                                         3916   3919   4125   4819
BbvI        !NNNNNNNNNNNNGCTGC       7    127   1828   2449   2907   3826
                                         4428   4617
BbvII       GAAGACNN!                0
BbvII       !NNNNNNGTCTTC            3    254   3123   5368
BcefI       ACGGCNNNNNNNNNNNN!       3   1338   1871   3999
BcefI       !NNNNNNNNNNNNNGCCGT      2   1111   1901
BcgI        !GCANNNNNNTCG            2   1903   3100
BcgI        !CGANNNNNNTGC            2    764   4905
BclI        T!GATCA                  4   1821   2745   2784   3038
BcnI        CC!SGG                   6    427    428   2571   3877   4573
                                         4924
BfaI        C!TAG                    9    309   1085   1960   1975   2592
                                         2848   3992   4245   4580
BfrI        C!TTAAG                  1   1935
BglI        GCCNNNN!NGGC             2   3328   4510
BglII       A!GATCT                  1    133
BinI        !NNNNNGATCC              7   2568   2796   4058   4144   4242
                                         4706   5027
BinI        GGATCNNNN!               6   2581   2809   3498   4146   4243
                                         5023
BlnI        C!CTAGG                  0
BmyI        GDGCH!C                  4   2780   3815   4976   5061
BpmI        !CTCCAG                  1   4474
BpmI        !CTGGAG                  1   3203
Bpu1102I    GC!TNAGC                 1   2440
BsaAI       YAC!GTR                  0
BsaBI       GATNN!NNATC              2   1770   3037
BsaHI       GR!CGYC                  7    521    983   1390   1566   1655
                                         4927   5309
BsaI        GGTCTCN!                 1   2694
BsaI        !NNNNNGAGACC             2   2559   4451
BsaJI       C!CNNGG                  6    426    427   2262   2569   2684
                                         3657
BseAI       T!CCGGA                  0
BsePI       !GCGCGC                  0
BsgI        !GTGCAG                  2   1239   2020
BsgI        !CTGCAC                  1   1694
BsiEI       CGRY!CG                  9    392   1331   2638   2797   3049
                                         3335   3837   4760   4909
BsiWI       C!GTACG                  1   1097
BsiYI       CCNNNNN!NNGG            12    384    427   1052   2321   2377
                                         2658   2685   3089   3519   3537
                                         3703   3982
BslI        CCNNNNN!NNGG            12    384    427   1052   2321   2377
                                         2658   2685   3089   3519   3537
                                         3703   3982
BsmAI       !GTCTC                   6    477   1039   1656   1668   2688
                                         5221
BsmAI       !GAGAC                   3   1545   2564   4456
BsmI        GAATGCN!                 4    285    504   1407   2857
BsmI        G!CATTC                  1   2950
Bsp1286I    GDGCH!C                  4   2780   3815   4976   5061
BspDI       AT!CGAT                  1   1709
BspEI       T!CCGGA                  0
BspHI       T!CATGA                  4     56   4217   5225   5330
BspMI       ACCTGCNNNN!              0
BspMI       !NNNNNNNNGCAGGT          1   3447
BspMII      T!CCGGA                  0
BsrFI       R!CCGGY                  7   1052   1330   1930   2706   3162
                                         3322   4470
BsrI        ACTGGN!                  5    349   3282   3905   3918   4866
BsrI        C!CAGT                   9   2316   2471   2597   3073   4030
                                         4436   4554   4597   5036
BssHII      G!CGCGC                  0
Bst1107I    GTA!TAC                  0
BstBI       TT!CGAA                  1   2656
BstEII      G!GTNACC                 1   2646
BstI        G!GATCC                  2   2573   2801
BstNI       CC!WGG                   5    385   3453   3525   3646   3659
BstPI       G!GTNACC                 1   2646
BstXI       CCANNNNN!NTGG            2   2604   2630
BstYI       R!GATCY                  9    133   2573   2801   4138   4149
                                         4235   4247   5015   5032
Bsu36I      CC!TNAGG                 0
CbiI        TT!CGAA                  1   2656
CcrI        C!TCGAG                  1   2641
CelII       GC!TNAGC                 1   2440
Cfr10I      R!CCGGY                  7   1052   1330   1930   2706   3162
                                         3322   4470
Cfr13I      G!GNCC                  11    154    430   2258   2468   3192
                                         3280   4432   4511   4528   4750
                                         5366
Cfr9I       C!CCGGG                  1    426
CfrI        Y!GGCCR                  6    179    346   1372   2635   3332
                                         4778
ClaI        AT!CGAT                  1   1709
CpoI        CG!GWCCG                 0
Csp45I      TT!CGAA                  1   2656
CspI        CG!GSCCG                 0
CvnI        CC!TNAGG                 0
DdeI        C!TNAG                  12    503   1628   1700   1803   2440
                                         2767   2774   3772   4181   4347
                                         4887   5313
DraI        TTT!AAA                  6   1170   2736   2997   4256   4275
                                         4967
DraII       RG!GNCCY                 2   2258   5366
DraIII      CACNNN!GTG               0
DrdI        GACNNNN!NNGTC            1   3605
DsaI        C!CRYGG                  1   2262
DsaV        !CCNGG                  11    383    425    426   2569   3451
                                         3523   3644   3657   3875   4571
                                         4922
EaeI        Y!GGCCR                  6    179    346   1372   2635   3332
                                         4778
EagI        C!GGCCG                  2   2635   3332
Eam1105I    GACNNN!NNGTC             1   4390
EarI        !CTCTTC                  4    481   1662   2274   5178
EarI        !GAAGAG                  0
Ecl136II    GAG!CTC                  1   2778
EclXI       C!GGCCG                  2   2635   3332
Eco105I     TAC!GTA                  0
Eco31I      GGTCTCN!                 1   2694
Eco31I      !NNNNNGAGACC             2   2559   4451
Eco47I      G!GWCC                   6   2258   2468   3192   3280   4528
                                         4750
Eco47III    AGC!GCT                  2   2438   3170
Eco52I      C!GGCCG                  2   2635   3332
Eco57I      !CTGAAG                  2   2203   4023
Eco57I      !CTTCAG                  1   5071
Eco81I      CC!TNAGG                 0
EcoNI       CCTNN!NNNAGG             0
EcoO109I    RG!GNCCY                 2   2258   5366
EcoO65I     G!GTNACC                 1   2646
EcoRI       G!AATTC                  1   2608
EcoRII      !CCWGG                   5    383   3451   3523   3644   3657
EcoRV       GAT!ATC                  1   2620
EcoT14I     C!CWWGG                  1   2262
EcoT22I     ATGCA!T                  1   1407
EcoT38I     GRGCY!C                  1   2780
EheI        GGC!GCC                  1   1391
Esp3I       CGTCTCN!                 2   1662   1674
Esp3I       !NNNNNGAGACG             1   1540
EspI        GC!TNAGC                 1   2440
FbaI        T!GATCA                  4   1821   2745   2784   3038
FinI        !GTCCC                   2   1998   3280
FinI        !GGGAC                   2   2466   3476
FokI        GGATGNNNNNNNNN!          2   3393   3438
FokI        !NNNNNNNNNNNNNCATCC      3   4356   4537   4824
FspI        TGC!GCA                  2   1681   4612
GdiII       !NNNNNYGGCCG             4   1366   2629   3326   4772
GdiII       CGGCCRN!                 2   2641   3338
GsuI        !CTCCAG                  1   4474
GsuI        !CTGGAG                  1   3203
HaeI        WGG!CCW                  8    181    348   2772   3313   3385
                                         3442   3523   3975
HaeII       RGCGC!Y                  5   1091   1393   2440   3172   3745
HgaI        GACGCNNNNN!              4   3346   3378   3608   4186
HgaI        !NNNNNNNNNNGCGTC         5    248    743   1128   1644   4916
HgiAI       GWGCW!C                  4   2780   3815   4976   5061
HgiEII      !ACCNNNNNNGGT            1   4077
Hin1I       GR!CGYC                  7    521    983   1390   1566   1655
                                         4927   5309
HincII      GTY!RAC                  5    633   1185   2936   3046   4931
HindIII     A!AGCTT                  0
HinfI       G!ANTC                   9     76    444   1508   2236   2694
                                         3245   3399   3868   4385
HpaI        GTT!AAC                  1   2936
HphI        GGTGANNNNNNNN!           2   4876   5117
HphI        !NNNNNNNTCACC            8    410    978   2640   2709   2715
                                         4233   4460   5082
KasI        G!GCGCC                  1   1389
KpnI        GGTAC!C                  1   2588
Ksp632I     CTCTTCN!                 4    488   1669   2281   5185
Ksp632I     !NNNNGAAGAG              0
KspI        CCGC!GG                  0
MaeI        C!TAG                    9    309   1085   1960   1975   2592
                                         2848   3992   4245   4580
MaeII       A!CGT                   12    171    521    983    992   1566
                                         2037   3294   3350   4200   4616
                                         4989   5309
MamI        GATNN!NNATC              2   1770   3037
MboII       GAAGANNNNNNNN!           5    507    550   1593   1990   4159
MboII       !NNNNNNNTCTTC           11    254    475   1656   2268   3123
                                         3394   4230   4985   5063   5172
                                         5368
McrI        !CGRYCG                  9    388   1327   2634   2793   3045
                                         3331   3833   4756   4905
MfeI        !CAATTG                  2   1417   2944
MflI        R!GATCY                  9    133   2573   2801   4138   4149
                                         4235   4247   5015   5032
MluI        A!CGCGT                  1   2700
MmeI        !TCCRAC                  2   3686   3870
MmeI        !GTYGGA                  1    993
MnlI        CCTCNNNNNNN!            12   1294   1585   2529   2689   2694
                                         2762   2801   3021   3268   3679
                                         4557   4763
MroI        T!CCGGA                  0
MscI        TGG!CCA                  2    181    348
MstI        TGC!GCA                  2   1681   4612
MstII       CC!TNAGG                 0
MunI        C!AATTG                  2   1418   2945
MvaI        CC!WGG                   5    385   3453   3525   3646   3659
NaeI        GCC!GGC                  2   3164   3324
NarI        GG!CGCC                  1   1390
NciI        CC!SGG                   6    427    428   2571   3877   4573
                                         4924
NcoI        C!CATGG                  1   2262
NdeI        CA!TATG                  1   2121
NgoMI       G!CCGGC                  2   3162   3322
NheI        G!CTAGC                  0
NotI        GC!GGCCGC                1   2635
NruI        TCG!CGA                  2    515   3367
NsiI        ATGCA!T                  1   1407
Nsp(7524)I  RCATG!Y                  7    600    779   1106   1122   1640
                                         2117   2245
Nsp(7524)V  TT!CGAA                  1   2656
NspBII      CMG!CKG                  7    934   1907   2203   2743   3839
                                         4084   5025
NspII       GDGCH!C                  4   2780   3815   4976   5061
NspIII      C!YCGRG                  2    426   2641
NspIV       G!GNCC                  11    154    430   2258   2468   3192
                                         3280   4432   4511   4528   4750
                                         5366
NunII       GG!CGCC                  1   1390
PacI        TTAAT!TAA                0
PaeR71      C!TCGAG                  1   2641
PflMI       CCANNNN!NTGG             1    384
PleI        GAGTCNNNN!               1   3876
PleI        !NNNNNGACTC              2    438   4379
PmaCI       CAC!GTG                  0
PmeI        GTTT!AAAC                1   2736
PmlI        CAC!GTG                  0
PpuMI       RG!GWCCY                 1   2258
PshAI       GACNN!NNGTC              1   3109
PstI        CTGCA!G                  2   2617   4635
PvuI        CGAT!CG                  3    392   2797   4760
PvuII       CAG!CTG                  2   1907   2203
RmaI        C!TAG                    9    309   1085   1960   1975   2592
                                         2848   3992   4245   4580
RsaI        GT!AC                    8    231   1099   1887   2118   2271
                                         2586   2705   4870
RsrI        G!AATTC                  1   2608
RsrII       CG!GWCCG                 0
SacI        GAGCT!C                  1   2780
SacII       CCGC!GG                  0
SalI        G!TCGAC                  1   3044
Sap1        GCTCTTCN!                0
Sau96I      G!GNCC                  11    154    430   2258   2468   3192
                                         3280   4432   4511   4528   4750
                                         5366
SauI        CC!TNAGG                 0
ScaI        AGT!ACT                  1   4870
ScrFI       CC!NGG                  11    385    427    428   2571   3453
                                         3525   3646   3659   3877   4573
                                         4924
SduI        GDGCH!C                  4   2780   3815   4976   5061
SecI        C!CNNGG                  6    426    427   2262   2569   2684
                                         3657
SexI        !CTCGAG                  1   2640
SfaNI       GCATCNNNNN!              6     74   1867   3428   3594   4646
                                         5086
SfaNI       !NNNNNNNNNGATGC          8     82    719   1799   1955   2879
                                         3041   3416   4837
SfcI        C!TYRAG                  9   1935   2400   2641   3057   3263
                                         3603   3865   4142   5010
SfiI        GGCCNNNN!NGGCC           0
SfuI        TT!CGAA                  1   2656
SgrAI       CR!CCGGYG                0
SinI        G!GWCC                   6   2258   2468   3192   3280   4528
                                         4750
SmaI        CCC!GGG                  1    428
SnaBI       TAC!GTA                  0
SnaI        !GTATAC                  0
SnoI        G!TGCAC                  2   3811   5057
SpeI        A!CTAGT                  2   1959   2591
SphI        GCATG!C                  1   1106
SplI        C!GTACG                  1   1097
SrfI        GCCC!GGGC                0
Sse8387I    CCTGCA!GG                0
SspI        AAT!ATT                  4   1434   1735   2054   5194
SstI        GAGCT!C                  1   2780
SstII       CCGC!GG                  0
SstIII      !ACGT                   12    170    520    982    991   1565
                                         2036   3293   3349   4199   4615
                                         4988   5308
StuI        AGG!CCT                  1   2772
StyI        C!CWWGG                  1   2262
StySJI      !GAGNNNNNNGTRC           0
StySJI      !GYACNNNNNNCTC           2     94   1885
SwaI        ATTT!AAAT                0
TaqI        T!CGA                   14    236    493   1152   1709   2212
                                         2642   2656   2692   2754   2793
                                         2797   3045   3597   5041
TaqII       GACCGANNNNNNNNNNN!       2   3063   4923
TaqII       !NNNNNNNNNTCGGTC         2   2676   4738
TaqII       CACCCANNNNNNNNNNN!       3   2329   2665   5076
TaqII       !NNNNNNNNNTGGGTG         1   5093
TfiI        G!AWTC                   6     76   1508   2236   2694   3245
                                         3399
Tsp45I      !GTSAC                   9    116    467    984   1567   2175
                                         2646   3273   4646   4857
Tth111I     GACN!NNGTC               0
Tth111II    CAARCANNNNNNNNNNN!       5    222   1610   2319   4087   4126
Tth111II    !NNNNNNNNNTGYTTG         5   1288   1439   1631   1665   4094
TthHB8I     T!CGA                   14    236    493   1152   1709   2212
                                         2642   2656   2692   2754   2793
                                         2797   3045   3597   5041
Van91I      CCANNNN!NTGG             1    384
VspI        AT!TAAT                  2     12   4562
XbaI        T!CTAGA                  0
XcmI        !CCANNNNNNNNNTCG         2   2199   3286
XcyI        C!CCGGG                  1    426
XhoI        C!TCGAG                  1   2641
XhoII       R!GATCY                  9    133   2573   2801   4138   4149
                                         4235   4247   5015   5032
XmaI        C!CCGGG                  1    426
XmaIII      C!GGCCG                  2   2635   3332
XmnI        GAANN!NNTTC              4     13    802   1873   4989
XorII       CGAT!CG                  3    392   2797   4760