File: pMTBiP/V5-His A 24-0427 Mode: Normal 1 - 3642 Table: DNASIS AVAIL Style: Circular Indication Mode: Actual Cutting Site Enzyme Name Sequence Count Cutting Position AatII GACGT!C 1 3579 Acc65I G!GTACC 1 923 AccI GT!MKAC 1 1388 AflII C!TTAAG 0 AflIII A!CRYGT 3 660 1043 1764 AgeI A!CCGGT 1 1049 AluI AG!CT 19 45 64 309 447 652 1121 1263 1407 1429 1524 1588 1706 1932 2022 2068 2325 2846 2946 3009 AlwI GGATCNNNN! 5 845 1152 2413 2510 3290 AlwI !NNNNNGATCC 7 832 1139 2325 2411 2509 2973 3294 AlwNI CAGNNN!CTG 1 2180 ApaI GGGCC!C 1 996 ApaLI G!TGCAC 3 178 2078 3324 ApoI R!AATTY 4 403 674 947 1224 AscI GG!CGCGCC 0 AseI AT!TAAT 3 1535 1594 2829 AvaI C!YCGRG 3 900 918 980 AvaII G!GWCC 2 2795 3017 AvrII C!CTAGG 0 BamHI G!GATCC 2 837 1144 BanI G!GYRCC 5 236 430 923 1508 2605 BanII GRGCY!C 2 996 1123 BbsI !GAAGAC 0 BbsI !GTCTTC 0 BbvI GCAGCNNNNNNNN! 6 54 808 2183 2186 2392 3086 BbvI !NNNNNNNNNNNNGCTGC 9 242 315 441 1250 1575 1656 1674 2093 2695 BclI T!GATCA 3 1088 1127 1381 BfaI C!TAG 7 444 931 987 1191 2259 2512 2847 BglI GCCNNNN!NGGC 3 252 879 2777 BglII A!GATCT 1 906 BpmI !CTGGAG 0 BpmI !CTCCAG 1 2741 Bpu1102I GC!TNAGC 0 BsaA1 YAC!GTR 0 BsaB1 GATNN!NNATC 1 1380 BsaH1 GR!CGYC 3 237 3194 3576 BsaI !NNNNNGAGACC 2 481 2718 BsaI GGTCTCN! 1 1037 BsaJI C!CNNGG 7 355 912 918 919 1027 1503 1924 BsgI GTGCAG! 0 BsgI !CTGCAC 1 655 BsiEI CGRY!CG 8 280 688 977 1140 1680 2104 3027 3176 BsiWI C!GTACG 0 BslI CCNNNNN!NNGG 10 54 613 918 1001 1028 1612 1786 1804 1970 2249 BsmAI !GTCTC 3 663 1031 3488 BsmAI !GAGAC 3 51 486 2723 BsmFI !NNNNNGTCCC 1 599 BsmFI GGGACN! 0 BsmI GAATGCN! 1 1200 BsmI G!CATTC 1 1293 Bsp1286I GDGCH!C 8 182 435 577 996 1123 2082 3243 3328 BspDI AT!CGAT 0 BspEI T!CCGGA 0 BspHI T!CATGA 3 2484 3492 3597 BspMI ACCTGCNNNN! 1 1400 BspMI !NNNNNNNNGCAGGT 0 BsrBI CCG!CTC 2 979 1456 BsrBI GAG!CGG 2 1697 3498 BsrDI !CATTGC 1 2892 BsrDI GCAATGNN! 1 2718 BsrFI R!CCGGY 4 683 687 1049 2737 BsrGI T!GTACA 0 BsrI ACTGGN! 4 874 2172 2185 3133 BsrI C!CAGT 10 366 392 398 936 1564 2297 2703 2821 2864 3303 BssHII G!CGCGC 0 Bst1107I GTA!TAC 0 BstBI TT!CGAA 1 999 BstEII G!GTNACC 0 BstNI CC!WGG 5 356 1504 1792 1913 1926 BstPI G!GTNACC 0 BstUI CG!CG 13 4 6 109 637 738 1045 1611 1613 1811 2392 2722 3215 3547 BstXI CCANNNNN!NTGG 2 943 969 BstYI R!GATCY 9 837 906 1144 2405 2416 2502 2514 3282 3299 Bsu36I CC!TNAGG 0 ClaI AT!CGAT 0 DdeI C!TNAG 9 172 818 1110 1117 2039 2448 2614 3154 3580 DpnI GA!TC 22 279 839 844 908 1090 1129 1139 1146 1383 2332 2407 2418 2426 2504 2516 2621 2962 2980 3026 3284 3301 3337 DpnII !GATC 22 277 837 842 906 1088 1127 1137 1144 1381 2330 2405 2416 2424 2502 2514 2619 2960 2978 3024 3282 3299 3335 DraI TTT!AAA 5 1079 1340 2523 2542 3234 DraIII CACNNN!GTG 0 DrdI GACNNNN!NNGTC 2 98 1872 DsaI C!CRYGG 1 912 EaeI Y!GGCCR 7 389 685 770 871 974 1603 3045 EagI C!GGCCG 2 685 974 Eam1105I GACNNN!NNGTC 1 2657 EarI !CTCTTC 3 290 1641 3445 EarI !GAAGAG 0 Eco47III AGC!GCT 0 Eco57I !CTGAAG 1 2290 Eco57I !CTTCAG 1 3338 EcoNI CCTNN!NNNAGG 0 EcoO109I RG!GNCCY 3 992 993 3633 EcoRI G!AATTC 1 947 EcoRII !CCWGG 5 354 1502 1790 1911 1924 EcoRV GAT!ATC 1 959 Esp3I !NNNNNGAGACG 1 46 Esp3I CGTCTCN! 1 669 Fnu4HI GC!NGC 25 43 152 256 329 455 736 797 974 977 1264 1589 1670 1688 1691 1809 1964 2107 2172 2175 2381 2709 3048 3075 3170 3399 FokI GGATGNNNNNNNNN! 3 91 335 437 FokI !NNNNNNNNNNNNNCATCC 4 579 2623 2804 3091 FspI TGC!GCA 3 259 561 2879 HaeII RGCGC!Y 3 240 1642 2012 HaeIII GG!CC 22 289 391 461 503 687 772 873 882 892 917 976 994 1115 1605 1779 1790 1808 2242 2700 2780 3047 3634 HgaI GACGCNNNNN! 2 1875 2453 HgaI !NNNNNNNNNNGCGTC 2 98 3183 HgiAI GWGCW!C 5 182 1123 2082 3243 3328 HhaI GCG!C 18 6 109 239 260 562 1548 1613 1641 1674 1944 2011 2111 2285 2394 2787 2880 3217 3549 HincII GTY!RAC 2 1279 1389 HindIII A!AGCTT 2 650 1405 HinfI G!ANTC 9 708 811 983 1037 1599 1664 1739 2135 2652 HpaI GTT!AAC 1 1279 HpaII C!CGG 17 49 83 500 684 688 919 1050 1482 1971 2118 2144 2334 2738 2772 2839 2949 3191 HphI GGTGANNNNNNNN! 5 25 34 717 3143 3384 HphI !NNNNNNNTCACC 5 1052 1058 2500 2727 3349 KasI G!GCGCC 1 236 KpnI GGTAC!C 1 927 MaeII A!CGT 7 375 411 662 2467 2883 3256 3576 MaeIII !GTNAC 13 57 348 368 439 1249 2120 2183 2299 2582 2913 2971 3124 3312 MboI !GATC 22 277 837 842 906 1088 1127 1137 1144 1381 2330 2405 2416 2424 2502 2514 2619 2960 2978 3024 3282 3299 3335 MboII GAAGANNNNNNNN! 1 2426 MboII !NNNNNNNTCTTC 7 284 624 1635 2497 3252 3330 3439 MluI A!CGCGT 1 1043 MnlI !NNNNNNNGAGG 11 695 982 1134 1314 1323 1612 1871 2195 2595 2676 3623 MnlI CCTCNNNNNNN! 13 40 300 481 903 1032 1037 1105 1144 1364 1663 1946 2824 3030 MscI TGG!CCA 1 772 MseI T!TAA 17 143 340 408 1078 1278 1339 1535 1594 2470 2522 2527 2541 2594 2829 2868 3233 3605 MspI C!CGG 17 49 83 500 684 688 919 1050 1482 1971 2118 2144 2334 2738 2772 2839 2949 3191 MunI C!AATTG 1 1288 NaeI GCC!GGC 2 685 689 NarI GG!CGCC 1 237 NciI CC!SGG 7 49 84 919 920 2144 2840 3191 NcoI C!CATGG 1 912 NdeI CA!TATG 2 185 533 NgoMI G!CCGGC 2 683 687 NheI G!CTAGC 0 NlaIII CATG! 15 42 566 573 916 1156 1403 1422 1768 2488 2979 2989 3067 3103 3496 3601 NlaIV GGN!NCC 17 238 432 478 504 682 839 925 994 1146 1510 1796 1835 2607 2701 2742 2953 3543 NotI GC!GGCCGC 1 974 NruI TCG!CGA 0 NsiI ATGCA!T 1 864 Nsp(7524)I RCATG!Y 4 42 566 1403 1768 NspBII CMG!CKG 7 115 309 1086 1588 2106 2351 3292 PacI TTAAT!TAA 0 PflMI CCANNNN!NTGG 0 PleI GAGTCNNNN! 2 991 2143 PleI !NNNNNGACTC 2 1658 2646 PmeI GTTT!AAAC 1 1079 PmlI CAC!GTG 0 Psp1406I AA!CGTT 3 411 2883 3256 PstI CTGCA!G 3 459 956 1397 PvuI CGAT!CG 3 280 1140 3027 PvuII CAG!CTG 2 309 1588 RsaI GT!AC 5 170 541 925 1048 3137 RsrII CG!GWCCG 0 SacI GAGCT!C 1 1123 SacII CCGC!GG 0 SalI G!TCGAC 1 1387 SapI !NNNNGAAGAGC 0 SapI GCTCTTCN! 1 1648 Sau3AI !GATC 22 277 837 842 906 1088 1127 1137 1144 1381 2330 2405 2416 2424 2502 2514 2619 2960 2978 3024 3282 3299 3335 Sau96I G!GNCC 10 287 502 916 992 993 2699 2778 2795 3017 3633 ScaI AGT!ACT 1 3137 ScrFI CC!NGG 12 49 84 356 919 920 1504 1792 1913 1926 2144 2840 3191 SfaNI GCATCNNNNN! 8 163 239 601 774 789 1861 2913 3353 SfaNI !NNNNNNNNNGATGC 4 69 199 1222 3104 SfcI C!TYRAG 5 980 1870 2132 2409 3277 SfiI GGCCNNNN!NGGCC 1 879 SfrI !GCCCGGGC 0 SgrAI CR!CCGGYG 0 SmaI CCC!GGG 1 920 SnaBI TAC!GTA 0 SpeI A!CTAGT 1 930 SphI GCATG!C 2 566 1403 SrfI GCCC!GGGC 0 Sse8387I CCTGCA!GG 1 1397 SspI AAT!ATT 1 3461 StuI AGG!CCT 1 1115 StyI C!CWWGG 1 912 SwaI ATTT!AAAT 0 TaqI T!CGA 11 672 711 981 999 1035 1097 1136 1140 1388 1864 3308 TfiI G!AWTC 5 708 811 1037 1599 1739 Tth111I GACN!NNGTC 0 XbaI T!CTAGA 1 986 XcmI !CCANNNNNNNNNTGG 0 XhoI C!TCGAG 1 980 XmaI C!CCGGG 1 918 XmaIII C!GGCCG 2 685 974 XmnI GAANN!NNTTC 2 628 3256