File: pMTBiP/V5-His C 24-0431
Mode: Normal              1 -    3638
Table: DNASIS AVAIL  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatII       GACGT!C                  1   3575
Acc65I      G!GTACC                  1    923
AccI        GT!MKAC                  1   1384
AflII       C!TTAAG                  0
AflIII      A!CRYGT                  3    660   1039   1760
AgeI        A!CCGGT                  1   1045
AluI        AG!CT                   19     45     64    309    447    652
                                         1117   1259   1403   1425   1520
                                         1584   1702   1928   2018   2064
                                         2321   2842   2942   3005
AlwI        GGATCNNNN!               5    845   1148   2409   2506   3286
AlwI        !NNNNNGATCC              7    832   1135   2321   2407   2505
                                         2969   3290
AlwNI       CAGNNN!CTG               1   2176
ApaI        GGGCC!C                  0
ApaLI       G!TGCAC                  3    178   2074   3320
ApoI        R!AATTY                  4    403    674    947   1220
AscI        GG!CGCGCC                0
AseI        AT!TAAT                  3   1531   1590   2825
AvaI        C!YCGRG                  3    900    918    980
AvaII       G!GWCC                   2   2791   3013
AvrII       C!CTAGG                  0
BamHI       G!GATCC                  2    837   1140
BanI        G!GYRCC                  5    236    430    923   1504   2601
BanII       GRGCY!C                  1   1119
BbsI        !GAAGAC                  0
BbsI        !GTCTTC                  0
BbvI        GCAGCNNNNNNNN!           6     54    808   2179   2182   2388
                                         3082
BbvI        !NNNNNNNNNNNNGCTGC       9    242    315    441   1246   1571
                                         1652   1670   2089   2691
BclI        T!GATCA                  3   1084   1123   1377
BfaI        C!TAG                    6    444    931   1187   2255   2508
                                         2843
BglI        GCCNNNN!NGGC             3    252    879   2773
BglII       A!GATCT                  1    906
BpmI        !CTGGAG                  0
BpmI        !CTCCAG                  1   2737
Bpu1102I    GC!TNAGC                 0
BsaA1       YAC!GTR                  0
BsaB1       GATNN!NNATC              1   1376
BsaH1       GR!CGYC                  3    237   3190   3572
BsaI        !NNNNNGAGACC             2    481   2714
BsaI        GGTCTCN!                 1   1033
BsaJI       C!CNNGG                  7    355    912    918    919   1023
                                         1499   1920
BsgI        GTGCAG!                  0
BsgI        !CTGCAC                  1    655
BsiEI       CGRY!CG                  8    280    688    977   1136   1676
                                         2100   3023   3172
BsiWI       C!GTACG                  0
BslI        CCNNNNN!NNGG            10     54    613    918    997   1024
                                         1608   1782   1800   1966   2245
BsmAI       !GTCTC                   3    663   1027   3484
BsmAI       !GAGAC                   3     51    486   2719
BsmFI       !NNNNNGTCCC              1    599
BsmFI       GGGACN!                  0
BsmI        GAATGCN!                 1   1196
BsmI        G!CATTC                  1   1289
Bsp1286I    GDGCH!C                  7    182    435    577   1119   2078
                                         3239   3324
BspDI       AT!CGAT                  0
BspEI       T!CCGGA                  0
BspHI       T!CATGA                  3   2480   3488   3593
BspMI       ACCTGCNNNN!              1   1396
BspMI       !NNNNNNNNGCAGGT          0
BsrBI       CCG!CTC                  2    979   1452
BsrBI       GAG!CGG                  2   1693   3494
BsrDI       !CATTGC                  1   2888
BsrDI       GCAATGNN!                1   2714
BsrFI       R!CCGGY                  4    683    687   1045   2733
BsrGI       T!GTACA                  0
BsrI        ACTGGN!                  4    874   2168   2181   3129
BsrI        C!CAGT                  10    366    392    398    936   1560
                                         2293   2699   2817   2860   3299
BssHII      G!CGCGC                  0
Bst1107I    GTA!TAC                  0
BstBI       TT!CGAA                  1    995
BstEII      G!GTNACC                 1    985
BstNI       CC!WGG                   5    356   1500   1788   1909   1922
BstPI       G!GTNACC                 1    985
BstUI       CG!CG                   13      4      6    109    637    738
                                         1041   1607   1609   1807   2388
                                         2718   3211   3543
BstXI       CCANNNNN!NTGG            2    943    969
BstYI       R!GATCY                  9    837    906   1140   2401   2412
                                         2498   2510   3278   3295
Bsu36I      CC!TNAGG                 0
ClaI        AT!CGAT                  0
DdeI        C!TNAG                   9    172    818   1106   1113   2035
                                         2444   2610   3150   3576
DpnI        GA!TC                   22    279    839    844    908   1086
                                         1125   1135   1142   1379   2328
                                         2403   2414   2422   2500   2512
                                         2617   2958   2976   3022   3280
                                         3297   3333
DpnII       !GATC                   22    277    837    842    906   1084
                                         1123   1133   1140   1377   2326
                                         2401   2412   2420   2498   2510
                                         2615   2956   2974   3020   3278
                                         3295   3331
DraI        TTT!AAA                  5   1075   1336   2519   2538   3230
DraIII      CACNNN!GTG               0
DrdI        GACNNNN!NNGTC            2     98   1868
DsaI        C!CRYGG                  1    912
EaeI        Y!GGCCR                  7    389    685    770    871    974
                                         1599   3041
EagI        C!GGCCG                  2    685    974
Eam1105I    GACNNN!NNGTC             1   2653
EarI        !CTCTTC                  3    290   1637   3441
EarI        !GAAGAG                  0
Eco47III    AGC!GCT                  0
Eco57I      !CTGAAG                  1   2286
Eco57I      !CTTCAG                  1   3334
EcoNI       CCTNN!NNNAGG             0
EcoO109I    RG!GNCCY                 1   3629
EcoRI       G!AATTC                  1    947
EcoRII      !CCWGG                   5    354   1498   1786   1907   1920
EcoRV       GAT!ATC                  1    959
Esp3I       !NNNNNGAGACG             1     46
Esp3I       CGTCTCN!                 1    669
Fnu4HI      GC!NGC                  25     43    152    256    329    455
                                          736    797    974    977   1260
                                         1585   1666   1684   1687   1805
                                         1960   2103   2168   2171   2377
                                         2705   3044   3071   3166   3395
FokI        GGATGNNNNNNNNN!          3     91    335    437
FokI        !NNNNNNNNNNNNNCATCC      4    579   2619   2800   3087
FspI        TGC!GCA                  3    259    561   2875
HaeII       RGCGC!Y                  3    240   1638   2008
HaeIII      GG!CC                   21    289    391    461    503    687
                                          772    873    882    892    917
                                          976   1111   1601   1775   1786
                                         1804   2238   2696   2776   3043
                                         3630
HgaI        GACGCNNNNN!              2   1871   2449
HgaI        !NNNNNNNNNNGCGTC         2     98   3179
HgiAI       GWGCW!C                  5    182   1119   2078   3239   3324
HhaI        GCG!C                   18      6    109    239    260    562
                                         1544   1609   1637   1670   1940
                                         2007   2107   2281   2390   2783
                                         2876   3213   3545
HincII      GTY!RAC                  2   1275   1385
HindIII     A!AGCTT                  2    650   1401
HinfI       G!ANTC                   8    708    811   1033   1595   1660
                                         1735   2131   2648
HpaI        GTT!AAC                  1   1275
HpaII       C!CGG                   17     49     83    500    684    688
                                          919   1046   1478   1967   2114
                                         2140   2330   2734   2768   2835
                                         2945   3187
HphI        GGTGANNNNNNNN!           5     25     34    717   3139   3380
HphI        !NNNNNNNTCACC            6    979   1048   1054   2496   2723
                                         3345
KasI        G!GCGCC                  1    236
KpnI        GGTAC!C                  1    927
MaeII       A!CGT                    7    375    411    662   2463   2879
                                         3252   3572
MaeIII      !GTNAC                  14     57    348    368    439    985
                                         1245   2116   2179   2295   2578
                                         2909   2967   3120   3308
MboI        !GATC                   22    277    837    842    906   1084
                                         1123   1133   1140   1377   2326
                                         2401   2412   2420   2498   2510
                                         2615   2956   2974   3020   3278
                                         3295   3331
MboII       GAAGANNNNNNNN!           1   2422
MboII       !NNNNNNNTCTTC            7    284    624   1631   2493   3248
                                         3326   3435
MluI        A!CGCGT                  1   1039
MnlI        !NNNNNNNGAGG            11    695    975   1130   1310   1319
                                         1608   1867   2191   2591   2672
                                         3619
MnlI        CCTCNNNNNNN!            13     40    300    481    903   1028
                                         1033   1101   1140   1360   1659
                                         1942   2820   3026
MscI        TGG!CCA                  1    772
MseI        T!TAA                   17    143    340    408   1074   1274
                                         1335   1531   1590   2466   2518
                                         2523   2537   2590   2825   2864
                                         3229   3601
MspI        C!CGG                   17     49     83    500    684    688
                                          919   1046   1478   1967   2114
                                         2140   2330   2734   2768   2835
                                         2945   3187
MunI        C!AATTG                  1   1284
NaeI        GCC!GGC                  2    685    689
NarI        GG!CGCC                  1    237
NciI        CC!SGG                   7     49     84    919    920   2140
                                         2836   3187
NcoI        C!CATGG                  1    912
NdeI        CA!TATG                  2    185    533
NgoMI       G!CCGGC                  2    683    687
NheI        G!CTAGC                  0
NlaIII      CATG!                   15     42    566    573    916   1152
                                         1399   1418   1764   2484   2975
                                         2985   3063   3099   3492   3597
NlaIV       GGN!NCC                 16    238    432    478    504    682
                                          839    925   1142   1506   1792
                                         1831   2603   2697   2738   2949
                                         3539
NotI        GC!GGCCGC                1    974
NruI        TCG!CGA                  0
NsiI        ATGCA!T                  1    864
Nsp(7524)I  RCATG!Y                  4     42    566   1399   1764
NspBII      CMG!CKG                  7    115    309   1082   1584   2102
                                         2347   3288
PacI        TTAAT!TAA                0
PflMI       CCANNNN!NTGG             0
PleI        GAGTCNNNN!               1   2139
PleI        !NNNNNGACTC              2   1654   2642
PmeI        GTTT!AAAC                1   1075
PmlI        CAC!GTG                  0
Psp1406I    AA!CGTT                  3    411   2879   3252
PstI        CTGCA!G                  3    459    956   1393
PvuI        CGAT!CG                  3    280   1136   3023
PvuII       CAG!CTG                  2    309   1584
RsaI        GT!AC                    5    170    541    925   1044   3133
RsrII       CG!GWCCG                 0
SacI        GAGCT!C                  1   1119
SacII       CCGC!GG                  0
SalI        G!TCGAC                  1   1383
SapI        !NNNNGAAGAGC             0
SapI        GCTCTTCN!                1   1644
Sau3AI      !GATC                   22    277    837    842    906   1084
                                         1123   1133   1140   1377   2326
                                         2401   2412   2420   2498   2510
                                         2615   2956   2974   3020   3278
                                         3295   3331
Sau96I      G!GNCC                   8    287    502    916   2695   2774
                                         2791   3013   3629
ScaI        AGT!ACT                  1   3133
ScrFI       CC!NGG                  12     49     84    356    919    920
                                         1500   1788   1909   1922   2140
                                         2836   3187
SfaNI       GCATCNNNNN!              8    163    239    601    774    789
                                         1857   2909   3349
SfaNI       !NNNNNNNNNGATGC          4     69    199   1218   3100
SfcI        C!TYRAG                  5    980   1866   2128   2405   3273
SfiI        GGCCNNNN!NGGCC           1    879
SfrI        !GCCCGGGC                0
SgrAI       CR!CCGGYG                0
SmaI        CCC!GGG                  1    920
SnaBI       TAC!GTA                  0
SpeI        A!CTAGT                  1    930
SphI        GCATG!C                  2    566   1399
SrfI        GCCC!GGGC                0
Sse8387I    CCTGCA!GG                1   1393
SspI        AAT!ATT                  1   3457
StuI        AGG!CCT                  1   1111
StyI        C!CWWGG                  1    912
SwaI        ATTT!AAAT                0
TaqI        T!CGA                   11    672    711    981    995   1031
                                         1093   1132   1136   1384   1860
                                         3304
TfiI        G!AWTC                   5    708    811   1033   1595   1735
Tth111I     GACN!NNGTC               0
XbaI        T!CTAGA                  0
XcmI        !CCANNNNNNNNNTGG         0
XhoI        C!TCGAG                  1    980
XmaI        C!CCGGG                  1    918
XmaIII      C!GGCCG                  2    685    974
XmnI        GAANN!NNTTC              2    628   3252