File: pMT/V5-His-TOPO 24-0591
Mode: Normal              1 -    3558
Table: DNASIS#  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatII       GACGT!C                  1   3495
Acc65I      G!GTACC                  1    850
AccI        GT!MKAC                  1   1304
AccII       CG!CG                   14      4      6    109    637    738
                                          941    992   1527   1529   1727
                                         2308   2638   3131   3463
AcyI        GR!CGYC                  3    237   3110   3492
AfaI        GT!AC                    5    170    541    852    995   3053
AflI        G!GWCC                   2   2711   2933
AflII       C!TTAAG                  1    882
AflIII      A!CRYGT                  3    660    990   1680
AgeI        A!CCGGT                  1    996
AhaI        CC!SGG                   5     49     84   2060   2756   3107
AhaII       GR!CGYC                  3    237   3110   3492
AhaIII      TTT!AAA                  5   1026   1256   2439   2458   3150
Alw44I      G!TGCAC                  3    178   1994   3240
AlwI        GGATCNNNN!               4    845   2329   2426   3206
AlwI        !NNNNNGATCC              6   1055   2241   2327   2425   2889
                                         3210
AlwNI       CAGNNN!CTG               1   2096
AocII       GDGCH!C                  7    182    435    577    939   1998
                                         3159   3244
AosI        TGC!GCA                  3    259    561   2795
AosII       GR!CGYC                  3    237   3110   3492
ApaI        GGGCC!C                  1    939
ApaLI       G!TGCAC                  3    178   1994   3240
ApoI        R!AATTY                  2    674   1140
ApyI        CC!WGG                   5    356   1420   1708   1829   1842
AquI        C!YCGRG                  1    923
AseI        AT!TAAT                  4    406   1451   1510   2745
AsnI        AT!TAAT                  4    406   1451   1510   2745
Asp700I     GAANN!NNTTC              3    407    628   3172
Asp718I     G!GTACC                  1    850
AspHI       GWGCW!C                  4    182   1998   3159   3244
AsuI        G!GNCC                   9    287    502    935    936   2615
                                         2694   2711   2933   3549
AsuII       TT!CGAA                  1    946
AvaI        C!YCGRG                  1    923
AvaII       G!GWCC                   2   2711   2933
AviII       TGC!GCA                  3    259    561   2795
AvrI        !CYCGRG                  1    922
BalI        TGG!CCA                  1    772
BanI        G!GYRCC                  5    236    430    850   1424   2521
BanII       GRGCY!C                  1    939
BbeI        GGCGC!C                  1    240
BbiII/AcyI  GR!CGYC                  3    237   3110   3492
BbvI        GCAGCNNNNNNNN!           6     54    808   2099   2102   2308
                                         3002
BbvI        !NNNNNNNNNNNNGCTGC       9    242    315    441   1166   1491
                                         1572   1590   2009   2611
BcefI       ACGGCNNNNNNNNNNNN!       2    404   2182
BcefI       !NNNNNNNNNNNNNGCCGT      1    447
BcgI        !CGANNNNNNTGC            1   3088
BclI        T!GATCA                  2   1035   1297
BcnI        CC!SGG                   5     49     84   2060   2756   3107
BfaI        C!TAG                    8    444    841    858    930   1107
                                         2175   2428   2763
BfrI        C!TTAAG                  1    882
BglI        GCCNNNN!NGGC             2    252   2693
BinI        !NNNNNGATCC              6   1055   2241   2327   2425   2889
                                         3210
BinI        GGATCNNNN!               4    845   2329   2426   3206
BmyI        GDGCH!C                  7    182    435    577    939   1998
                                         3159   3244
BpmI        !CTCCAG                  1   2657
BsaBI       GATNN!NNATC              2    842   1296
BsaHI       GR!CGYC                  3    237   3110   3492
BsaI        GGTCTCN!                 1    984
BsaI        !NNNNNGAGACC             2    481   2634
BsaJI       C!CNNGG                  5    355    939    974   1419   1840
BsgI        !CTGCAC                  1    655
BsiEI       CGRY!CG                  7    280    688    920   1596   2020
                                         2943   3092
BsiYI       CCNNNNN!NNGG             8     54    613    975   1528   1702
                                         1720   1886   2165
BslI        CCNNNNN!NNGG             8     54    613    975   1528   1702
                                         1720   1886   2165
BsmAI       !GTCTC                   3    663    978   3404
BsmAI       !GAGAC                   3     51    486   2639
BsmI        GAATGCN!                 1   1116
BsmI        G!CATTC                  1   1209
Bsp1286I    GDGCH!C                  7    182    435    577    939   1998
                                         3159   3244
BspHI       T!CATGA                  3   2400   3408   3513
BspMI       ACCTGCNNNN!              1   1316
BspWI       GCNNNNN!NNGC            14    186    236    243    252    282
                                          572   1325   1424   1468   1552
                                         1619   1733   2305   2693
BsrFI       R!CCGGY                  4    683    687    996   2653
BsrI        ACTGGN!                  3   2088   2101   3049
BsrI        C!CAGT                  10    366    392    398    863   1480
                                         2213   2619   2737   2780   3219
BstBI       TT!CGAA                  1    946
BstNI       CC!WGG                   5    356   1420   1708   1829   1842
BstUI       CG!CG                   14      4      6    109    637    738
                                          941    992   1527   1529   1727
                                         2308   2638   3131   3463
BstXI       CCANNNNN!NTGG            2    870    912
BstYI       R!GATCY                  8    837   1060   2321   2332   2418
                                         2430   3198   3215
CbiI        TT!CGAA                  1    946
CcrI        C!TCGAG                  1    923
Cfr10I      R!CCGGY                  4    683    687    996   2653
Cfr13I      G!GNCC                   9    287    502    935    936   2615
                                         2694   2711   2933   3549
CfrI        Y!GGCCR                  6    389    685    770    917   1519
                                         2961
Csp45I      TT!CGAA                  1    946
DdeI        C!TNAG                   8    172    818   1057   1955   2364
                                         2530   3070   3496
DraI        TTT!AAA                  5   1026   1256   2439   2458   3150
DraII       RG!GNCCY                 2    935   3549
DrdI        GACNNNN!NNGTC            2     98   1788
DsaI        C!CRYGG                  1    939
DsaV        !CCNGG                  10     47     82    354   1418   1706
                                         1827   1840   2058   2754   3105
EaeI        Y!GGCCR                  6    389    685    770    917   1519
                                         2961
EagI        C!GGCCG                  2    685    917
Eam1105I    GACNNN!NNGTC             1   2573
EarI        !CTCTTC                  3    290   1557   3361
EclXI       C!GGCCG                  2    685    917
Eco31I      GGTCTCN!                 1    984
Eco31I      !NNNNNGAGACC             2    481   2634
Eco47I      G!GWCC                   2   2711   2933
Eco52I      C!GGCCG                  2    685    917
Eco57I      !CTGAAG                  1   2206
Eco57I      !CTTCAG                  1   3254
EcoO109I    RG!GNCCY                 2    935   3549
EcoRII      !CCWGG                   5    354   1418   1706   1827   1840
EcoRV       GAT!ATC                  1    902
EcoT38I     GRGCY!C                  1    939
EheI        GGC!GCC                  1    238
Esp3I       CGTCTCN!                 1    669
Esp3I       !NNNNNGAGACG             1     46
FbaI        T!GATCA                  2   1035   1297
FinI        !GTCCC                   1    604
FokI        GGATGNNNNNNNNN!          3     91    335    437
FokI        !NNNNNNNNNNNNNCATCC      4    579   2539   2720   3007
FspI        TGC!GCA                  3    259    561   2795
GdiII       !NNNNNYGGCCG             3    679    911   2955
GdiII       CGGCCRN!                 4    395    691    923   1525
GsuI        !CTCCAG                  1   2657
HaeI        WGG!CCW                  4    772   1695   1706   2158
HaeII       RGCGC!Y                  3    240   1558   1928
HgaI        GACGCNNNNN!              2   1791   2369
HgaI        !NNNNNNNNNNGCGTC         2     98   3099
HgiAI       GWGCW!C                  4    182   1998   3159   3244
HgiEII      !ACCNNNNNNGGT            2    181   2260
Hin1I       GR!CGYC                  3    237   3110   3492
HincII      GTY!RAC                  2   1195   1305
HindIII     A!AGCTT                  2    650   1321
HinfI       G!ANTC                   9    708    811    926    984   1515
                                         1580   1655   2051   2568
HpaI        GTT!AAC                  1   1195
HphI        GGTGANNNNNNNN!           5     25     34    717   3059   3300
HphI        !NNNNNNNTCACC            5    999   1005   2416   2643   3265
KasI        G!GCGCC                  1    236
KpnI        GGTAC!C                  1    854
Ksp632I     CTCTTCN!                 3    297   1564   3368
KspI        CCGC!GG                  1    942
MaeI        C!TAG                    8    444    841    858    930   1107
                                         2175   2428   2763
MaeII       A!CGT                    6    375    662   2383   2799   3172
                                         3492
MaeIII      !GTNAC                  13     57    348    368    439   1165
                                         2036   2099   2215   2498   2829
                                         2887   3040   3228
MamI        GATNN!NNATC              2    842   1296
MboII       GAAGANNNNNNNN!           1   2342
MboII       !NNNNNNNTCTTC            7    284    624   1551   2413   3168
                                         3246   3355
McrI        !CGRYCG                  7    276    684    916   1592   2016
                                         2939   3088
MfeI        !CAATTG                  1   1203
MflI        R!GATCY                  8    837   1060   2321   2332   2418
                                         2430   3198   3215
MluI        A!CGCGT                  1    990
MmeI        !TCCRAC                  2   1869   2053
MnlI        CCTCNNNNNNN!            11     40    300    481    979    984
                                         1052   1280   1579   1862   2740
                                         2946
MnlI        !NNNNNNNGAGG            10    695    925   1230   1239   1528
                                         1787   2111   2511   2592   3539
MscI        TGG!CCA                  1    772
MstI        TGC!GCA                  3    259    561   2795
MunI        C!AATTG                  1   1204
MvaI        CC!WGG                   5    356   1420   1708   1829   1842
MvnI        CG!CG                   14      4      6    109    637    738
                                          941    992   1527   1529   1727
                                         2308   2638   3131   3463
NaeI        GCC!GGC                  2    685    689
NarI        GG!CGCC                  1    237
NciI        CC!SGG                   5     49     84   2060   2756   3107
NdeI        CA!TATG                  2    185    533
NgoMI       G!CCGGC                  2    683    687
NlaIII      CATG!                   14     42    566    573   1072   1319
                                         1338   1684   2404   2895   2905
                                         2983   3019   3412   3517
NlaIV       GGN!NCC                 15    238    432    478    504    682
                                          852    937   1426   1712   1751
                                         2523   2617   2658   2869   3459
NotI        GC!GGCCGC                1    917
Nsp(7524)I  RCATG!Y                  4     42    566   1319   1684
Nsp(7524)V  TT!CGAA                  1    946
NspBII      CMG!CKG                  8    115    309    941   1033   1504
                                         2022   2267   3208
NspII       GDGCH!C                  7    182    435    577    939   1998
                                         3159   3244
NspIII      C!YCGRG                  1    923
NspIV       G!GNCC                   9    287    502    935    936   2615
                                         2694   2711   2933   3549
NunII       GG!CGCC                  1    237
PaeR71      C!TCGAG                  1    923
PleI        GAGTCNNNN!               2    934   2059
PleI        !NNNNNGACTC              2   1574   2562
PmeI        GTTT!AAAC                1   1026
PstI        CTGCA!G                  3    459    899   1313
PvuI        CGAT!CG                  2    280   2943
PvuII       CAG!CTG                  2    309   1504
RmaI        C!TAG                    8    444    841    858    930   1107
                                         2175   2428   2763
RsaI        GT!AC                    5    170    541    852    995   3053
SacII       CCGC!GG                  1    942
SalI        G!TCGAC                  1   1303
Sap1        GCTCTTCN!                1   1564
Sau96I      G!GNCC                   9    287    502    935    936   2615
                                         2694   2711   2933   3549
ScaI        AGT!ACT                  1   3053
ScrFI       CC!NGG                  10     49     84    356   1420   1708
                                         1829   1842   2060   2756   3107
SduI        GDGCH!C                  7    182    435    577    939   1998
                                         3159   3244
SecI        C!CNNGG                  5    355    939    974   1419   1840
SexI        !CTCGAG                  1    922
SfaNI       GCATCNNNNN!              8    163    239    601    774    789
                                         1777   2829   3269
SfaNI       !NNNNNNNNNGATGC          4     69    199   1138   3020
SfcI        C!TYRAG                  6    882    923   1786   2048   2325
                                         3193
SfuI        TT!CGAA                  1    946
SinI        G!GWCC                   2   2711   2933
SnoI        G!TGCAC                  3    178   1994   3240
SpeI        A!CTAGT                  1    857
SphI        GCATG!C                  2    566   1319
Sse8387I    CCTGCA!GG                1   1313
SspI        AAT!ATT                  1   3377
SstII       CCGC!GG                  1    942
SstIII      !ACGT                    6    374    661   2382   2798   3171
                                         3491
StySJI      !GAGNNNNNNGTRC           1    159
TaqI        T!CGA                    9    672    711    924    946    982
                                         1044   1304   1780   3224
TaqII       GACCGANNNNNNNNNNN!       1   3106
TaqII       !NNNNNNNNNTCGGTC         3    966   1582   2921
TaqII       CACCCANNNNNNNNNNN!       1   3259
TaqII       !NNNNNNNNNTGGGTG         1   3276
TfiI        G!AWTC                   5    708    811    984   1515   1655
ThaI        CG!CG                   14      4      6    109    637    738
                                          941    992   1527   1529   1727
                                         2308   2638   3131   3463
Tsp45I      !GTSAC                   5     57    368    439   2829   3040
TspEI       !AATT                   14    403    407    628    674    874
                                          890   1140   1204   1360   1377
                                         1452   2440   2746   3001
Tth111II    CAARCANNNNNNNNNNN!       2   2270   2309
Tth111II    !NNNNNNNNNTGYTTG         1   2277
TthHB8I     T!CGA                    9    672    711    924    946    982
                                         1044   1304   1780   3224
VspI        AT!TAAT                  4    406   1451   1510   2745
XbaI        T!CTAGA                  2    840    929
XhoI        C!TCGAG                  1    923
XhoII       R!GATCY                  8    837   1060   2321   2332   2418
                                         2430   3198   3215
XmaIII      C!GGCCG                  2    685    917
XmnI        GAANN!NNTTC              3    407    628   3172
XorII       CGAT!CG                  2    280   2943