(Linear) (Circular) MAPSORT of: pPIC3.seq check: 8735 from: 1 to: 7764 With 203 enzymes: * February 22, 1996 14:40 .. AatII G_ACGT'C Cuts at: 7589 7589 Size: 7764 AccI GT'mk_AC Cuts at: 2919 3558 4750 5054 5546 2919 Size: 639 1192 304 492 5137 Fragments arranged by size: 5137 1192 639 492 304 AceIII CAGCTCnnnnnnn'nnnn_ Cuts at: 4076 5259 5426 5726 6966 4076 Size: 1183 167 300 1240 4874 Fragments arranged by size: 4874 1240 1183 300 167 AciI C'CG_C Cuts at: 320 656 975 979 1344 1486 1594 1603 1606 Size: 336 319 4 365 142 108 9 3 Cuts at: 1606 1763 1884 2637 3671 3819 3852 3918 4383 Size: 157 121 753 1034 148 33 66 465 Cuts at: 4383 4536 4539 4542 4648 4926 5076 5305 5447 Size: 153 3 3 106 278 150 229 142 Cuts at: 5447 5486 5496 5538 5563 5601 5614 5640 5657 Size: 39 10 42 25 38 13 26 17 Cuts at: 5657 5700 5707 5728 5819 5847 5974 5993 6114 Size: 43 7 21 91 28 127 19 121 Cuts at: 6114 6224 6359 6368 6730 6821 7012 7058 7179 Size: 110 135 9 362 91 191 46 121 Cuts at: 7179 7223 7300 7409 7508 7555 320 Size: 44 77 109 99 47 529 Fragments arranged by size: 1034 753 529 465 365 362 336 319 278 229 191 157 153 150 148 142 142 135 127 121 121 121 110 109 108 106 99 91 91 77 66 47 46 44 43 42 39 38 33 28 26 25 21 19 17 13 10 9 9 7 4 3 3 3 AflIII A'CryG_T Cuts at: 5774 5774 Size: 7764 AgeI A'CCGG_T Cuts at: 1041 1041 Size: 7764 AluI AG'CT Cuts at: 206 317 797 837 874 1180 1207 1324 1336 Size: 111 480 40 37 306 27 117 12 Cuts at: 1336 2157 2191 2250 2460 2742 2832 3252 3270 Size: 821 34 59 210 282 90 420 18 Cuts at: 3270 4066 4120 4156 5249 5416 5435 5716 5942 Size: 796 54 36 1093 167 19 281 226 Cuts at: 5942 6078 6335 6856 6956 7019 7682 7697 206 Size: 136 257 521 100 63 663 15 273 Fragments arranged by size: 1093 821 796 663 521 480 420 306 282 281 273 257 226 210 167 136 117 111 100 90 63 59 54 40 37 36 34 27 19 18 15 12 AlwI GGATCnnnn'n_ Cuts at: 933 946 1688 1789 1977 2043 2291 3349 3981 Size: 13 742 101 188 66 248 1058 632 Cuts at: 3981 4328 5057 5254 6335 6421 6423 6519 6520 Size: 347 729 197 1081 86 2 96 1 Cuts at: 6520 6983 7300 7304 933 Size: 463 317 4 1393 Fragments arranged by size: 1393 1081 1058 742 729 632 463 347 317 248 197 188 101 96 86 66 13 4 2 1 Alw21I G_wGCw'C Cuts at: 208 626 1585 4549 5103 5594 6092 7253 7338 Size: 418 959 2964 554 491 498 1161 85 Cuts at: 7338 208 Size: 634 Fragments arranged by size: 2964 1161 959 634 554 498 491 418 85 Alw44I G'TGCA_C Cuts at: 622 5590 6088 7334 622 Size: 4968 498 1246 1052 Fragments arranged by size: 4968 1246 1052 498 AlwNI CAG_nnn'CTG Cuts at: 2439 2463 6190 2439 Size: 24 3727 4013 Fragments arranged by size: 4013 3727 24 ApaBI GCA_nnnnn'TGC Cuts at: 2464 4225 5599 2464 Size: 1761 1374 4629 Fragments arranged by size: 4629 1761 1374 ApoI r'AATT_y Cuts at: 746 963 2750 3455 3697 4288 4724 5299 5350 Size: 217 1787 705 242 591 436 575 51 Cuts at: 5350 746 Size: 3160 Fragments arranged by size: 3160 1787 705 591 575 436 242 217 51 AvaII G'GwC_C Cuts at: 60 131 2406 3947 4009 4078 4947 6805 7027 Size: 71 2275 1541 62 69 869 1858 222 Cuts at: 7027 60 Size: 797 Fragments arranged by size: 2275 1858 1541 869 797 222 71 69 62 AvrII C'CTAG_G Cuts at: 969 969 Size: 7764 BamHI G'GATC_C Cuts at: 938 938 Size: 7764 BanI G'GyrC_C Cuts at: 1381 1424 1928 1967 2471 2540 4371 4392 4506 Size: 43 504 39 504 69 1831 21 114 Cuts at: 4506 4877 6615 1381 Size: 371 1738 2530 Fragments arranged by size: 2530 1831 1738 504 504 371 114 69 43 39 21 BanII G_rGCy'C Cuts at: 208 4433 4447 208 Size: 4225 14 3525 Fragments arranged by size: 4225 3525 14 BbsI GAAGACnn'nnnn_ Cuts at: 1044 2840 7645 1044 Size: 1796 4805 1163 Fragments arranged by size: 4805 1796 1163 BbvI GCAGCnnnnnnnn'nnnn_ Cuts at: 121 784 1216 1518 1800 2394 2469 4165 4176 Size: 663 432 302 282 594 75 1696 11 Cuts at: 4176 4560 5425 5522 5666 5684 6103 6193 6196 Size: 384 865 97 144 18 419 90 3 Cuts at: 6196 6402 6705 6894 7096 121 Size: 206 303 189 202 789 Fragments arranged by size: 1696 865 789 663 594 432 419 384 303 302 282 206 202 189 144 97 90 75 18 11 3 BccI CCATC Cuts at: 45 169 1799 2372 2545 2863 3472 3664 4418 Size: 124 1630 573 173 318 609 192 754 Cuts at: 4418 4511 6704 6828 7115 45 Size: 93 2193 124 287 694 Fragments arranged by size: 2193 1630 754 694 609 573 318 287 192 173 124 124 93 Bce83I CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 925 2272 4067 4182 4303 4685 5293 5343 5865 Size: 1347 1795 115 121 382 608 50 522 Cuts at: 5865 6163 6404 7272 925 Size: 298 241 868 1417 Fragments arranged by size: 1795 1417 1347 868 608 522 382 298 241 121 115 50 BcefI ACGGCnnnnnnnnnnnn'n_ Cuts at: 508 4195 4284 4568 6276 508 Size: 3687 89 284 1708 1996 Fragments arranged by size: 3687 1996 1708 284 89 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1309 1343 7172 7206 1309 Size: 34 5829 34 1867 Fragments arranged by size: 5829 1867 34 34 BclI T'GATC_A Cuts at: 740 1189 2001 4141 740 Size: 449 812 2140 4363 Fragments arranged by size: 4363 2140 812 449 BfaI C'TA_G Cuts at: 106 970 1051 1535 1774 2308 2482 3120 3909 Size: 864 81 484 239 534 174 638 789 Cuts at: 3909 3963 4006 5136 6269 6522 6857 106 Size: 54 43 1130 1133 253 335 1013 Fragments arranged by size: 1133 1130 1013 864 789 638 534 484 335 253 239 174 81 54 43 BglI GCCn_nnn'nGGC Cuts at: 278 2329 6787 278 Size: 2051 4458 1255 Fragments arranged by size: 4458 2051 1255 BglII A'GATC_T Cuts at: 1 5362 1 Size: 5361 2403 BmgI GkGCCC Cuts at: 1027 5280 1027 Size: 4253 3511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 326 1978 3358 3496 3547 6737 326 Size: 1652 1380 138 51 3190 1353 Fragments arranged by size: 3190 1652 1380 1353 138 51 Bpu1102I GC'TnA_GC Cuts at: 588 588 Size: 7764 BsaI GGTCTCn'nnnn_ Cuts at: 3827 4675 6728 3827 Size: 848 2053 4863 Fragments arranged by size: 4863 2053 848 BsaAI yAC'GTr Cuts at: 959 5528 959 Size: 4569 3195 BsaBI GATnn'nnATC Cuts at: 1058 1214 5264 1058 Size: 156 4050 3558 Fragments arranged by size: 4050 3558 156 BsaHI Gr'CG_yC Cuts at: 2472 3688 4372 4393 4507 7204 7586 2472 Size: 1216 684 21 114 2697 382 2650 Fragments arranged by size: 2697 2650 1216 684 382 114 21 BsaJI C'CnnG_G Cuts at: 276 617 947 969 1420 1434 2141 2227 2467 Size: 341 330 22 451 14 707 86 240 Cuts at: 2467 2703 3085 3226 3241 3373 3943 3969 4486 Size: 236 382 141 15 132 570 26 517 Cuts at: 4486 4492 5166 5934 276 Size: 6 674 768 2106 Fragments arranged by size: 2106 768 707 674 570 517 451 382 341 330 240 236 141 132 86 26 22 15 14 6 BsaWI w'CCGG_w Cuts at: 1041 1931 3585 4701 5980 6127 6958 1041 Size: 890 1654 1116 1279 147 831 1847 Fragments arranged by size: 1847 1654 1279 1116 890 831 147 BsaXI ACnnnnnCTCC Cuts at: 137 137 Size: 7764 BsbI CAACAC Cuts at: 155 1993 3435 4221 5492 7210 155 Size: 1838 1442 786 1271 1718 709 Fragments arranged by size: 1838 1718 1442 1271 786 709 BscGI CCCGT Cuts at: 1572 1671 1897 4486 5470 6101 6447 6668 6692 Size: 99 226 2589 984 631 346 221 24 Cuts at: 6692 7214 1572 Size: 522 2122 Fragments arranged by size: 2589 2122 984 631 522 346 226 221 99 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 131 139 1268 1950 3012 4058 4720 4723 131 Size: 8 1129 682 1062 1046 662 3 3172 Fragments arranged by size: 3172 1129 1062 1046 682 662 8 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 4172 2135 Size: 2037 5727 BsiI C'TCGT_G Cuts at: 5102 5947 7331 7638 5102 Size: 845 1384 307 5228 Fragments arranged by size: 5228 1384 845 307 BsiEI CG_ry'CG Cuts at: 979 1594 5690 6114 7037 7186 979 Size: 615 4096 424 923 149 1557 Fragments arranged by size: 4096 1557 923 615 424 149 BslI CCnn_nnn'nnGG Cuts at: 190 282 349 662 948 974 1486 1577 1677 Size: 92 67 313 286 26 512 91 100 Cuts at: 1677 1911 2614 2959 3045 3365 3394 4006 4075 Size: 234 703 345 86 320 29 612 69 Cuts at: 4075 4491 4492 4538 4568 4586 4956 5425 5796 Size: 416 1 46 30 18 370 469 371 Cuts at: 5796 5814 5980 6259 190 Size: 18 166 279 1695 Fragments arranged by size: 1695 703 612 512 469 416 371 370 345 320 313 286 279 234 166 100 92 91 86 69 67 46 30 29 26 18 18 1 BsmI GAATG_Cn' Cuts at: 312 567 1082 2019 4650 312 Size: 255 515 937 2631 3426 Fragments arranged by size: 3426 2631 937 515 255 BsmAI GTCTCn'nnnn_ Cuts at: 602 685 2248 2717 2732 3472 3827 4675 4758 Size: 83 1563 469 15 740 355 848 83 Cuts at: 4758 4797 5417 6728 7504 602 Size: 39 620 1311 776 862 Fragments arranged by size: 1563 1311 862 848 776 740 620 469 355 83 83 39 15 BsmBI CGTCTCn'nnnn_ Cuts at: 2732 5417 2732 Size: 2685 5079 BsmFI GGGACnnnnnnnnnn'nnnn_ Cuts at: 1714 1810 3062 3147 4510 4803 1714 Size: 96 1252 85 1363 293 4675 Fragments arranged by size: 4675 1363 1252 293 96 85 BsoFI GC'n_GC Cuts at: 110 798 976 979 1205 1532 1603 1606 1789 Size: 688 178 3 226 327 71 3 183 Cuts at: 1789 2383 2458 4154 4165 4537 4540 4574 5414 Size: 594 75 1696 11 372 3 34 840 Cuts at: 5414 5511 5564 5680 5698 5701 5819 5974 6117 Size: 97 53 116 18 3 118 155 143 Cuts at: 6117 6182 6185 6391 6719 6908 7058 7085 7180 Size: 65 3 206 328 189 150 27 95 Cuts at: 7180 7409 110 Size: 229 465 Fragments arranged by size: 1696 840 688 594 465 372 328 327 229 226 206 189 183 178 155 150 143 118 116 97 95 75 71 65 53 34 27 18 11 3 3 3 3 3 Bsp24I GACnnnnnnTGGnnnnnnn_nnnnn' Cuts at: 2695 2727 3556 3588 4339 4371 6267 6299 6445 Size: 32 829 32 751 32 1896 32 146 Cuts at: 6445 6477 7571 7603 7691 7723 2695 Size: 32 1094 32 88 32 2736 Fragments arranged by size: 2736 1896 1094 829 751 146 88 32 32 32 32 32 32 32 Bsp1286I G_dGCh'C Cuts at: 208 626 1029 1585 4433 4447 4549 5103 5282 Size: 418 403 556 2848 14 102 554 179 Cuts at: 5282 5594 6092 7253 7338 208 Size: 312 498 1161 85 634 Fragments arranged by size: 2848 1161 634 556 554 498 418 403 312 179 102 85 14 BspEI T'CCGG_A Cuts at: 3585 3585 Size: 7764 BspGI CTGGAC Cuts at: 2406 4947 2406 Size: 2541 5223 BspLU11I A'CATG_T Cuts at: 5774 5774 Size: 7764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 4224 4224 Size: 7764 BsrI ACTG_Gn' Cuts at: 184 496 861 1515 1610 1732 2400 3564 3880 Size: 312 365 654 95 122 668 1164 316 Cuts at: 3880 4573 5071 5521 5554 6182 6195 6307 6713 Size: 693 498 450 33 628 13 112 406 Cuts at: 6713 6831 6874 7143 7313 184 Size: 118 43 269 170 635 Fragments arranged by size: 1164 693 668 654 635 628 498 450 406 365 316 312 269 170 122 118 112 95 43 33 13 BsrBI GAG'CGG Cuts at: 4926 5707 7508 4926 Size: 781 1801 5182 Fragments arranged by size: 5182 1801 781 BsrDI GCAATG_nn' Cuts at: 1758 3342 6728 6902 1758 Size: 1584 3386 174 2620 Fragments arranged by size: 3386 2620 1584 174 BsrFI r'CCGG_y Cuts at: 1041 1465 4359 4368 6747 1041 Size: 424 2894 9 2379 2058 Fragments arranged by size: 2894 2379 2058 424 9 BsrGI T'GTAC_A Cuts at: 4039 4039 Size: 7764 Bst1107I GTA'TAC Cuts at: 5547 5547 Size: 7764 BstXI CCAn_nnnn'nTGG Cuts at: 706 2378 2509 706 Size: 1672 131 5961 Fragments arranged by size: 5961 1672 131 BstYI r'GATC_y Cuts at: 1 938 1680 1794 1982 4333 5362 6415 6426 Size: 937 742 114 188 2351 1029 1053 11 Cuts at: 6426 6512 6524 7292 7309 1 Size: 86 12 768 17 456 Fragments arranged by size: 2351 1053 1029 937 768 742 456 188 114 86 17 12 11 Bsu36I CC'TnA_GG Cuts at: 1829 1829 Size: 7764 Cac8I GCn'nGC Cuts at: 208 1100 1182 1322 1529 1536 1620 2078 2189 Size: 892 82 140 207 7 84 458 111 Cuts at: 2189 2330 2483 3920 4361 4391 4433 4481 4522 Size: 141 153 1437 441 30 42 48 41 Cuts at: 4522 4928 5705 5791 5828 6388 6779 6912 208 Size: 406 777 86 37 560 391 133 1060 Fragments arranged by size: 1437 1060 892 777 560 458 441 406 391 207 153 141 140 133 111 86 84 82 48 42 41 37 30 7 CjeI CCAnnnnnnGTnnnnnnnnn'nnnnnn_ Cuts at: 450 483 2258 2291 2689 2722 2777 2810 2927 Size: 33 1775 33 398 33 55 33 117 Cuts at: 2927 2960 3160 3193 3418 3451 3519 3550 3552 Size: 33 200 33 225 33 68 31 2 Cuts at: 3552 3583 4333 4366 4972 5005 5581 5614 5787 Size: 31 750 33 606 33 576 33 173 Cuts at: 5787 5820 6261 6294 6339 6342 6372 6375 6439 Size: 33 441 33 45 3 30 3 64 Cuts at: 6439 6472 7565 7598 7685 7718 450 Size: 33 1093 33 87 33 496 Fragments arranged by size: 1775 1093 750 606 576 496 441 398 225 200 173 117 87 68 64 55 45 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 31 31 30 3 3 2 CjePI GAnnnnnnnTGGnnnnnnn'nnnnnn_ Cuts at: 30 63 123 156 1260 1293 1596 1629 1798 Size: 33 60 33 1104 33 303 33 169 Cuts at: 1798 1831 2507 2540 2689 2722 3213 3246 3550 Size: 33 676 33 149 33 491 33 304 Cuts at: 3550 3583 4333 4366 4696 4729 5007 5040 5227 Size: 33 750 33 330 33 278 33 187 Cuts at: 5227 5260 6261 6293 6294 6326 6439 6472 6559 Size: 33 1001 32 1 32 113 33 87 Cuts at: 6559 6592 7031 7064 7565 7598 7685 7718 30 Size: 33 439 33 501 33 87 33 76 Fragments arranged by size: 1104 1001 750 676 501 491 439 330 304 303 278 187 169 149 113 87 87 76 60 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 32 32 1 ClaI AT'CG_AT Cuts at: 1329 7690 1329 Size: 6361 1403 CviJI rG'Cy Cuts at: 182 195 206 234 258 272 317 353 396 Size: 13 11 28 24 14 45 36 43 Cuts at: 396 495 728 797 837 874 978 1102 1180 Size: 99 233 69 40 37 104 124 78 Cuts at: 1180 1195 1207 1324 1336 1455 1479 1602 1637 Size: 15 12 117 12 119 24 123 35 Cuts at: 1637 1791 1811 2076 2157 2191 2214 2250 2323 Size: 154 20 265 81 34 23 36 73 Cuts at: 2323 2352 2451 2460 2660 2742 2784 2832 2892 Size: 29 99 9 200 82 42 48 60 Cuts at: 2892 3003 3100 3231 3252 3270 3369 3378 3398 Size: 111 97 131 21 18 99 9 20 Cuts at: 3398 3582 3617 3642 4066 4120 4156 4182 4298 Size: 184 35 25 424 54 36 26 116 Cuts at: 4298 4359 4431 4445 4483 4491 4555 4573 5070 Size: 61 72 14 38 8 64 18 497 Cuts at: 5070 5165 5249 5416 5435 5468 5513 5553 5697 Size: 95 84 167 19 33 45 40 144 Cuts at: 5697 5716 5789 5800 5818 5844 5942 6078 6083 Size: 19 73 11 18 26 98 136 5 Cuts at: 6083 6108 6187 6252 6263 6306 6335 6698 6710 Size: 25 79 65 11 43 29 363 12 Cuts at: 6710 6751 6777 6781 6790 6856 6946 6956 7019 Size: 41 26 4 9 66 90 10 63 Cuts at: 7019 7057 7644 7682 7697 182 Size: 38 587 38 15 249 Fragments arranged by size: 587 497 424 363 265 249 233 200 184 167 154 144 136 131 124 123 119 117 116 111 104 99 99 99 98 97 95 90 84 82 81 79 78 73 73 72 69 66 65 64 63 61 60 54 48 45 45 43 43 42 41 40 40 38 38 38 37 36 36 36 35 35 34 33 29 29 28 26 26 26 25 25 24 24 23 21 20 20 19 19 18 18 18 15 15 14 14 13 12 12 12 11 11 11 10 9 9 9 8 5 4 CviRI TG'CA Cuts at: 122 310 624 675 1098 1320 1555 1872 2032 Size: 188 314 51 423 222 235 317 160 Cuts at: 2032 2091 2115 2152 2565 2582 2682 2722 2809 Size: 59 24 37 413 17 100 40 87 Cuts at: 2809 3023 3201 3258 3930 4105 4153 4218 4233 Size: 214 178 57 672 175 48 65 15 Cuts at: 4233 4520 4524 4586 5413 5592 6090 6386 6721 Size: 287 4 62 827 179 498 296 335 Cuts at: 6721 6811 6910 7004 7092 7336 122 Size: 90 99 94 88 244 550 Fragments arranged by size: 827 672 550 498 423 413 335 317 314 296 287 244 235 222 214 188 179 178 175 160 100 99 94 90 88 87 65 62 59 57 51 48 40 37 24 17 15 4 DdeI C'TnA_G Cuts at: 358 467 588 995 1012 1089 1829 2266 2426 Size: 109 121 407 17 77 740 437 160 Cuts at: 2426 2495 2552 3005 3381 3824 4121 5584 6049 Size: 69 57 453 376 443 297 1463 465 Cuts at: 6049 6458 6624 7164 7590 358 Size: 409 166 540 426 532 Fragments arranged by size: 1463 740 540 532 465 453 443 437 426 409 407 376 297 166 160 121 109 77 69 57 17 DpnI GA'TC Cuts at: 3 450 742 913 940 1191 1655 1682 1796 Size: 447 292 171 27 251 464 27 114 Cuts at: 1796 1984 2003 2037 2285 2777 3356 3975 4062 Size: 188 19 34 248 492 579 619 87 Cuts at: 4062 4143 4335 4426 5064 5146 5261 5364 6342 Size: 81 192 91 638 82 115 103 978 Cuts at: 6342 6417 6428 6436 6514 6526 6631 6972 6990 Size: 75 11 8 78 12 105 341 18 Cuts at: 6990 7036 7294 7311 7347 7731 3 Size: 46 258 17 36 384 36 Fragments arranged by size: 978 638 619 579 492 464 447 384 341 292 258 251 248 192 188 171 115 114 105 103 91 87 82 81 78 75 46 36 36 34 27 27 19 18 17 12 11 8 DraI TTT'AAA Cuts at: 413 5200 5342 6533 6552 7244 413 Size: 4787 142 1191 19 692 933 Fragments arranged by size: 4787 1191 933 692 142 19 DraIII CAC_nnn'GTG Cuts at: 3365 3365 Size: 7764 DrdI GACnn_nn'nnGTC Cuts at: 5220 5469 5882 5220 Size: 249 413 7102 Fragments arranged by size: 7102 413 249 DrdII GAACCA Cuts at: 476 860 2370 476 Size: 384 1510 5870 Fragments arranged by size: 5870 1510 384 DsaI C'CryG_G Cuts at: 947 3373 4486 947 Size: 2426 1113 4225 Fragments arranged by size: 4225 2426 1113 EaeI y'GGCC_r Cuts at: 493 976 1600 1809 3396 4357 4489 7055 493 Size: 483 624 209 1587 961 132 2566 1202 Fragments arranged by size: 2566 1587 1202 961 624 483 209 132 EagI C'GGCC_G Cuts at: 976 976 Size: 7764 Eam1105I GACnn_n'nnGTC Cuts at: 2261 4739 6667 2261 Size: 2478 1928 3358 Fragments arranged by size: 3358 2478 1928 EarI CTCTTCn'nnn_ Cuts at: 149 1286 3628 5658 7462 149 Size: 1137 2342 2030 1804 451 Fragments arranged by size: 2342 2030 1804 1137 451 EciI TCCGCC Cuts at: 5848 5994 6822 5848 Size: 146 828 6790 Fragments arranged by size: 6790 828 146 Eco47III AGC'GCT Cuts at: 1539 4454 1539 Size: 2915 4849 Eco57I CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1267 2299 3025 3712 4314 4640 4975 6322 7334 Size: 1032 726 687 602 326 335 1347 1012 Cuts at: 7334 1267 Size: 1697 Fragments arranged by size: 1697 1347 1032 1012 726 687 602 335 326 EcoNI CCTnn'n_nnAGG Cuts at: 3043 4584 4954 3043 Size: 1541 370 5853 Fragments arranged by size: 5853 1541 370 EcoO109I rG'GnC_Cy Cuts at: 131 3947 4482 7643 131 Size: 3816 535 3161 252 Fragments arranged by size: 3816 3161 535 252 EcoRI G'AATT_C Cuts at: 963 963 Size: 7764 EcoRII 'CCwGG_ Cuts at: 267 276 1434 2226 2402 2466 2645 2702 3095 Size: 9 1158 792 176 64 179 57 393 Cuts at: 3095 3144 3226 3612 3942 3968 5800 5921 5934 Size: 49 82 386 330 26 1832 121 13 Cuts at: 5934 267 Size: 2097 Fragments arranged by size: 2097 1832 1158 792 393 386 330 179 176 121 82 64 57 49 26 13 9 EcoRV GAT'ATC Cuts at: 1492 5317 1492 Size: 3825 3939 FauI CCCGCnnnn'nn_ Cuts at: 663 1479 3911 4655 4919 5479 5489 663 Size: 816 2432 744 264 560 10 2938 Fragments arranged by size: 2938 2432 816 744 560 264 10 FokI GGATGnnnnnnnnn'nnnn_ Cuts at: 31 38 440 678 1081 1403 1451 2531 3939 Size: 7 402 238 403 322 48 1080 1408 Cuts at: 3939 3963 4104 5462 6633 6814 7101 7759 31 Size: 24 141 1358 1171 181 287 658 36 Fragments arranged by size: 1408 1358 1171 1080 658 403 402 322 287 238 181 141 48 36 24 7 FspI TGC'GCA Cuts at: 1567 6889 1567 Size: 5322 2442 GdiII y'GGCC_G Cuts at: 493 976 1600 4357 4489 7055 493 Size: 483 624 2757 132 2566 1202 Fragments arranged by size: 2757 2566 1202 624 483 132 HaeI wGG'CCw Cuts at: 1811 2660 3003 3398 5789 5800 6252 1811 Size: 849 343 395 2391 11 452 3323 Fragments arranged by size: 3323 2391 849 452 395 343 11 HaeII r_GCGC'y Cuts at: 1541 2475 3969 4375 4396 4456 4510 5652 6022 Size: 934 1494 406 21 60 54 1142 370 Cuts at: 6022 1541 Size: 3283 Fragments arranged by size: 3283 1494 1142 934 406 370 60 54 21 HaeIII GG'CC Cuts at: 272 396 495 978 1479 1602 1811 2323 2660 Size: 124 99 483 501 123 209 512 337 Cuts at: 2660 3003 3398 3617 4359 4483 4491 4555 5789 Size: 343 395 219 742 124 8 64 1234 Cuts at: 5789 5800 5818 6252 6710 6790 7057 7644 272 Size: 11 18 434 458 80 267 587 392 Fragments arranged by size: 1234 742 587 512 501 483 458 434 395 392 343 337 267 219 209 124 124 123 99 80 64 18 11 8 HgaI GACGCnnnnn'nnnnn_ Cuts at: 3696 4357 4596 5469 5885 6463 7193 7733 3696 Size: 661 239 873 416 578 730 540 3727 Fragments arranged by size: 3727 873 730 661 578 540 416 239 HgiEII ACCnnnnnnGGT Cuts at: 5599 6360 5599 Size: 761 7003 HhaI G_CG'C Cuts at: 594 1408 1540 1568 2474 3968 4374 4395 4455 Size: 814 132 28 906 1494 406 21 60 Cuts at: 4455 4509 5377 5480 5510 5651 5684 5954 6021 Size: 54 868 103 30 141 33 270 67 Cuts at: 6021 6121 6295 6404 6797 6890 7227 7559 594 Size: 100 174 109 393 93 337 332 799 Fragments arranged by size: 1494 906 868 814 799 406 393 337 332 270 174 141 132 109 103 100 93 67 60 54 33 30 28 21 Hin4I GAbnnnnnvTC Cuts at: 6 136 203 562 1008 1639 1939 2260 3139 Size: 130 67 359 446 631 300 321 879 Cuts at: 3139 3344 4738 4768 5314 6666 6740 7682 6 Size: 205 1394 30 546 1352 74 942 88 Fragments arranged by size: 1394 1352 942 879 631 546 446 359 321 300 205 130 88 74 67 30 HincII GTy'rAC Cuts at: 1879 2920 7208 1879 Size: 1041 4288 2435 Fragments arranged by size: 4288 2435 1041 HindIII A'AGCT_T Cuts at: 872 1322 1334 872 Size: 450 12 7302 Fragments arranged by size: 7302 450 12 HinfI G'AnT_C Cuts at: 703 1054 1689 2221 2232 2858 3066 3560 3636 Size: 351 635 532 11 626 208 494 76 Cuts at: 3636 4095 4318 4590 4752 5028 5674 5749 6145 Size: 459 223 272 162 276 646 75 396 Cuts at: 6145 6662 7700 703 Size: 517 1038 767 Fragments arranged by size: 1038 767 646 635 626 532 517 494 459 396 351 276 272 223 208 162 76 75 11 HpaI GTT'AAC Cuts at: 1879 1879 Size: 7764 HphI GGTGAnnnnnnn_n' Cuts at: 1423 1777 2216 2890 4358 4403 4652 5396 5405 Size: 354 439 674 1468 45 249 744 9 Cuts at: 5405 6510 6737 7153 7359 7394 1423 Size: 1105 227 416 206 35 1793 Fragments arranged by size: 1793 1468 1105 744 674 439 416 354 249 227 206 45 35 9 KpnI G_GTAC'C Cuts at: 1932 2544 1932 Size: 612 7152 MaeII A'CG_T Cuts at: 733 958 1839 1909 2517 2725 2760 2793 3012 Size: 225 881 70 608 208 35 33 219 Cuts at: 3012 3169 5001 5204 5345 5527 6477 6893 7266 Size: 157 1832 203 141 182 950 416 373 Cuts at: 7266 7586 733 Size: 320 911 Fragments arranged by size: 1832 950 911 881 608 416 373 320 225 219 208 203 182 157 141 70 35 33 MaeIII 'GTnAC_ Cuts at: 1127 1429 1517 1835 2387 2419 2896 3122 3170 Size: 302 88 318 552 32 477 226 48 Cuts at: 3170 3421 3541 3800 4085 4994 5428 5523 6130 Size: 251 120 259 285 909 434 95 607 Cuts at: 6130 6193 6309 6592 6923 6981 7134 7322 1127 Size: 63 116 283 331 58 153 188 1569 Fragments arranged by size: 1569 909 607 552 477 434 331 318 302 285 283 259 251 226 188 153 120 116 95 88 63 58 48 32 MboII GAAGAnnnnnnn_n' Cuts at: 136 921 1049 1159 1273 1306 2025 2167 2840 Size: 785 128 110 114 33 719 142 673 Cuts at: 2840 2974 3061 3229 3645 3718 3900 4434 4646 Size: 134 87 168 416 73 182 534 212 Cuts at: 4646 5645 6436 6507 7262 7340 7449 7645 136 Size: 999 791 71 755 78 109 196 255 Fragments arranged by size: 999 791 785 755 719 673 534 416 255 212 196 182 168 142 134 128 114 110 109 87 78 73 71 33 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 72 1527 1614 3190 4244 5989 6173 72 Size: 1455 87 1576 1054 1745 184 1663 Fragments arranged by size: 1745 1663 1576 1455 1054 184 87 MnlI CCTCnnnnnn_n' Cuts at: 88 144 152 160 276 342 1021 1059 1289 Size: 56 8 8 116 66 679 38 230 Cuts at: 1289 1430 1490 1838 1928 2261 2485 2650 2828 Size: 141 60 348 90 333 224 165 178 Cuts at: 2828 2990 2993 3170 3284 3593 4079 4091 4347 Size: 162 3 177 114 309 486 12 256 Cuts at: 4347 4566 4660 4698 4701 4950 5105 5268 5273 Size: 219 94 38 3 249 155 163 5 Cuts at: 5273 5381 5411 5673 5882 5956 6206 6606 6687 Size: 108 30 262 209 74 250 400 81 Cuts at: 6687 6834 7040 7634 88 Size: 147 206 594 218 Fragments arranged by size: 679 594 486 400 348 333 309 262 256 250 249 230 224 219 218 209 206 178 177 165 163 162 155 147 141 116 114 108 94 90 81 74 66 60 56 38 38 30 12 8 8 5 3 3 MscI TGG'CCA Cuts at: 1811 3398 1811 Size: 1587 6177 MseI T'TA_A Cuts at: 412 582 750 808 884 894 986 1299 1339 Size: 170 168 58 76 10 92 313 40 Cuts at: 1339 1361 1704 1721 1752 1878 2790 4110 5199 Size: 22 343 17 31 126 912 1320 1089 Cuts at: 5199 5207 5234 5341 5555 6480 6532 6537 6551 Size: 8 27 107 214 925 52 5 14 Cuts at: 6551 6604 6839 6878 7243 7615 7663 412 Size: 53 235 39 365 372 48 513 Fragments arranged by size: 1320 1089 925 912 513 372 365 343 313 235 214 170 168 126 107 92 76 58 53 52 48 40 39 31 27 22 17 14 10 8 5 MslI CAynn'nnrTG Cuts at: 76 1763 6919 7078 7437 76 Size: 1687 5156 159 359 403 Fragments arranged by size: 5156 1687 403 359 159 MspI C'CG_G Cuts at: 619 1042 1466 1475 1932 3586 4345 4360 4369 Size: 423 424 9 457 1654 759 15 9 Cuts at: 4369 4492 4702 5420 5454 5981 6128 6154 6344 Size: 123 210 718 34 527 147 26 190 Cuts at: 6344 6748 6782 6849 6959 7201 619 Size: 404 34 67 110 242 1182 Fragments arranged by size: 1654 1182 759 718 527 457 424 423 404 242 210 190 147 123 110 67 34 34 26 15 9 9 MspA1I CmG'CkG Cuts at: 1207 1886 4156 5486 6116 6361 7302 1207 Size: 679 2270 1330 630 245 941 1669 Fragments arranged by size: 2270 1669 1330 941 679 630 245 MunI C'AATT_G Cuts at: 864 2804 2923 3752 864 Size: 1940 119 829 4876 Fragments arranged by size: 4876 1940 829 119 MwoI GCnn_nnn'nnGC Cuts at: 278 1201 1471 1537 1544 1602 1624 1658 2329 Size: 923 270 66 7 58 22 34 671 Cuts at: 2329 2457 2463 2478 2595 3375 3909 4224 4369 Size: 128 6 15 117 780 534 315 145 Cuts at: 4369 4380 4401 4592 5598 5648 5713 5827 6399 Size: 11 21 191 1006 50 65 114 572 Cuts at: 6399 6787 6907 278 Size: 388 120 1135 Fragments arranged by size: 1135 1006 923 780 671 572 534 388 315 270 191 145 128 120 117 114 66 65 58 50 34 22 21 15 11 7 6 NarI GG'CG_CC Cuts at: 2472 4372 4393 4507 2472 Size: 1900 21 114 5729 Fragments arranged by size: 5729 1900 114 21 NciI CC's_GG Cuts at: 619 1476 4493 5420 5455 6154 6850 7201 619 Size: 857 3017 927 35 699 696 351 1182 Fragments arranged by size: 3017 1182 927 857 699 696 351 35 NcoI C'CATG_G Cuts at: 947 3373 947 Size: 2426 5338 NdeI CA'TA_TG Cuts at: 5597 5597 Size: 7764 NgoAIV G'CCGG_C Cuts at: 4359 4359 Size: 7764 NheI G'CTAG_C Cuts at: 1534 2481 1534 Size: 947 6817 NlaIII _CATG' Cuts at: 248 292 384 741 951 1004 1660 2245 2336 Size: 44 92 357 210 53 656 585 91 Cuts at: 2336 2749 3377 4451 4524 4721 5413 5518 5778 Size: 413 628 1074 73 197 692 105 260 Cuts at: 5778 6498 6989 6999 7077 7113 7506 7611 7674 Size: 720 491 10 78 36 393 105 63 Cuts at: 7674 7707 248 Size: 33 305 Fragments arranged by size: 1074 720 692 656 628 585 491 413 393 357 305 260 210 197 105 105 92 91 78 73 63 53 44 36 33 10 NlaIV GGn'nCC Cuts at: 273 428 861 940 1383 1426 1636 1930 1969 Size: 155 433 79 443 43 210 294 39 Cuts at: 1969 2473 2542 2641 3948 4373 4394 4484 4508 Size: 504 69 99 1307 425 21 90 24 Cuts at: 4508 4879 5806 5845 6617 6711 6752 6963 7553 Size: 371 927 39 772 94 41 211 590 Cuts at: 7553 273 Size: 484 Fragments arranged by size: 1307 927 772 590 504 484 443 433 425 371 294 211 210 155 99 94 90 79 69 43 41 39 39 24 21 NotI GC'GGCC_GC Cuts at: 976 976 Size: 7764 NsiI A_TGCA'T Cuts at: 677 677 Size: 7764 NspI r_CATG'y Cuts at: 2336 4524 5413 5778 2336 Size: 2188 889 365 4322 Fragments arranged by size: 4322 2188 889 365 NspV TT'CG_AA Cuts at: 933 4865 933 Size: 3932 3832 Pfl1108I TCGTAG Cuts at: 2084 5253 6685 2084 Size: 3169 1432 3163 Fragments arranged by size: 3169 3163 1432 PflMI CCAn_nnn'nTGG Cuts at: 190 948 3365 190 Size: 758 2417 4589 Fragments arranged by size: 4589 2417 758 PleI GAGTCnnnn'n_ Cuts at: 2852 3554 3644 4089 4326 4598 4746 5668 6153 Size: 702 90 445 237 272 148 922 485 Cuts at: 6153 6656 7694 2852 Size: 503 1038 2922 Fragments arranged by size: 2922 1038 922 702 503 485 445 272 237 148 90 PmeI GTTT'AAAC Cuts at: 413 413 Size: 7764 Psp5II rG'GwC_Cy Cuts at: 131 3947 131 Size: 3816 3948 Psp1406I AA'CG_TT Cuts at: 733 2793 5001 5204 6893 7266 733 Size: 2060 2208 203 1689 373 1231 Fragments arranged by size: 2208 2060 1689 1231 373 203 PstI C_TGCA'G Cuts at: 6912 6912 Size: 7764 PvuI CG_AT'CG Cuts at: 7037 7037 Size: 7764 PvuII CAG'CTG Cuts at: 1207 4156 1207 Size: 2949 4815 RcaI T'CATG_A Cuts at: 737 4447 6494 7502 7607 737 Size: 3710 2047 1008 105 894 Fragments arranged by size: 3710 2047 1008 894 105 RleAI CCCACAnnnnnnnnn_nnn' Cuts at: 353 2499 3375 353 Size: 2146 876 4742 Fragments arranged by size: 4742 2146 876 RsaI GT'AC Cuts at: 1175 1290 1470 1908 1930 1997 2055 2129 2542 Size: 115 180 438 22 67 58 74 413 Cuts at: 2542 3180 3609 4041 5331 5582 7147 1175 Size: 638 429 432 1290 251 1565 1792 Fragments arranged by size: 1792 1565 1290 638 438 432 429 413 251 180 115 74 67 58 22 SacI G_AGCT'C Cuts at: 208 208 Size: 7764 SalI G'TCGA_C Cuts at: 2918 2918 Size: 7764 SapI GCTCTTCn'nnn_ Cuts at: 5658 5658 Size: 7764 Sau96I G'GnC_C Cuts at: 60 131 271 395 1477 2322 2406 3616 3947 Size: 71 140 124 1082 845 84 1210 331 Cuts at: 3947 4009 4078 4482 4947 6709 6788 6805 7027 Size: 62 69 404 465 1762 79 17 222 Cuts at: 7027 7643 60 Size: 616 181 Fragments arranged by size: 1762 1210 1082 845 616 465 404 331 222 181 140 124 84 79 71 69 62 17 Sau3AI 'GATC_ Cuts at: 1 448 740 911 938 1189 1653 1680 1794 Size: 447 292 171 27 251 464 27 114 Cuts at: 1794 1982 2001 2035 2283 2775 3354 3973 4060 Size: 188 19 34 248 492 579 619 87 Cuts at: 4060 4141 4333 4424 5062 5144 5259 5362 6340 Size: 81 192 91 638 82 115 103 978 Cuts at: 6340 6415 6426 6434 6512 6524 6629 6970 6988 Size: 75 11 8 78 12 105 341 18 Cuts at: 6988 7034 7292 7309 7345 7729 1 Size: 46 258 17 36 384 36 Fragments arranged by size: 978 638 619 579 492 464 447 384 341 292 258 251 248 192 188 171 115 114 105 103 91 87 82 81 78 75 46 36 36 34 27 27 19 18 17 12 11 8 ScaI AGT'ACT Cuts at: 7147 7147 Size: 7764 ScrFI CC'n_GG Cuts at: 269 278 619 1436 1476 2228 2404 2468 2647 Size: 9 341 817 40 752 176 64 179 Cuts at: 2647 2704 3097 3146 3228 3614 3944 3970 4493 Size: 57 393 49 82 386 330 26 523 Cuts at: 4493 5420 5455 5802 5923 5936 6154 6850 7201 Size: 927 35 347 121 13 218 696 351 Cuts at: 7201 269 Size: 832 Fragments arranged by size: 927 832 817 752 696 523 393 386 351 347 341 330 218 179 176 121 82 64 57 49 40 35 26 13 9 SfaNI GCATCnnnnn'nnnn_ Cuts at: 1085 1429 1518 1542 1779 2181 2552 2620 2988 Size: 344 89 24 237 402 371 68 368 Cuts at: 2988 3114 3771 3917 4360 4372 4972 5300 5440 Size: 126 657 146 443 12 600 328 140 Cuts at: 5440 5575 5611 5651 5871 6923 7114 7363 1085 Size: 135 36 40 220 1052 191 249 1486 Fragments arranged by size: 1486 1052 657 600 443 402 371 368 344 328 249 237 220 191 146 140 135 126 89 68 40 36 24 12 SfcI C'TryA_G Cuts at: 1443 2767 3951 6039 6230 6908 1443 Size: 1324 1184 2088 191 678 2299 Fragments arranged by size: 2299 2088 1324 1184 678 191 SgrAI Cr'CCGG_yG Cuts at: 4368 4368 Size: 7764 SnaBI TAC'GTA Cuts at: 959 959 Size: 7764 SphI G_CATG'C Cuts at: 4524 4524 Size: 7764 SspI AAT'ATT Cuts at: 2414 7471 2414 Size: 5057 2707 StuI AGG'CCT Cuts at: 3003 3003 Size: 7764 StyI C'CwwG_G Cuts at: 947 969 2141 3085 3241 3373 5166 947 Size: 22 1172 944 156 132 1793 3545 Fragments arranged by size: 3545 1793 1172 944 156 132 22 SunI C'GTAC_G Cuts at: 1173 1906 1173 Size: 733 7031 TaqI T'CG_A Cuts at: 171 933 1246 1329 1644 1687 2025 2208 2919 Size: 762 313 83 315 43 338 183 711 Cuts at: 2919 3486 4059 4126 4610 4755 4865 5319 5874 Size: 567 573 67 484 145 110 454 555 Cuts at: 5874 7318 7690 7762 171 Size: 1444 372 72 173 Fragments arranged by size: 1444 762 711 573 567 555 484 454 372 338 315 313 183 173 145 110 83 72 67 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 172 2621 5676 7015 7200 7353 7370 172 Size: 2449 3055 1339 185 153 17 566 Fragments arranged by size: 3055 2449 1339 566 185 153 17 TfiI G'AwT_C Cuts at: 703 1054 1689 2221 2232 3066 5028 5749 703 Size: 351 635 532 11 834 1962 721 2718 Fragments arranged by size: 2718 1962 834 721 635 532 351 11 ThaI CG'CG Cuts at: 981 1652 5375 5377 5480 5821 6402 6732 7225 Size: 671 3723 2 103 341 581 330 493 Cuts at: 7225 7557 981 Size: 332 1188 Fragments arranged by size: 3723 1188 671 581 493 341 332 330 103 2 TseI GCwGC Cuts at: 110 798 1205 1532 1789 2383 2458 4154 4165 Size: 688 407 327 257 594 75 1696 11 Cuts at: 4165 4574 5414 5511 5680 5698 6117 6182 6185 Size: 409 840 97 169 18 419 65 3 Cuts at: 6185 6391 6719 6908 7085 110 Size: 206 328 189 177 789 Fragments arranged by size: 1696 840 789 688 594 419 409 407 328 327 257 206 189 177 169 97 75 65 18 11 3 Tsp45I 'GTsAC_ Cuts at: 1429 1517 2419 2896 3122 3170 3421 3541 3800 Size: 88 902 477 226 48 251 120 259 Cuts at: 3800 4994 5428 5523 6923 7134 1429 Size: 1194 434 95 1400 211 2059 Fragments arranged by size: 2059 1400 1194 902 477 434 259 251 226 211 120 95 88 48 Tsp509I 'AATT_ Cuts at: 218 746 848 864 926 963 983 987 1363 Size: 528 102 16 62 37 20 4 376 Cuts at: 1363 1556 1718 2131 2179 2572 2632 2750 2804 Size: 193 162 413 48 393 60 118 54 Cuts at: 2804 2923 2936 2986 3016 3026 3280 3455 3482 Size: 119 13 50 30 10 254 175 27 Cuts at: 3482 3627 3697 3752 4073 4203 4288 4724 5299 Size: 145 70 55 321 130 85 436 575 Cuts at: 5299 5350 6534 6840 7095 7660 7664 218 Size: 51 1184 306 255 565 4 318 Fragments arranged by size: 1184 575 565 528 436 413 393 376 321 318 306 255 254 193 175 162 145 130 119 118 102 85 70 62 60 55 54 51 50 48 37 30 27 20 16 13 10 4 4 Tth111I GACn'n_nGTC Cuts at: 5521 5521 Size: 7764 Tth111II CAArCAnnnnnnnnn_nn' Cuts at: 281 533 1795 1991 3430 3477 5038 6364 6371 Size: 252 1262 196 1439 47 1561 1326 7 Cuts at: 6371 6403 7663 281 Size: 32 1260 382 Fragments arranged by size: 1561 1439 1326 1262 1260 382 252 196 47 32 7 UbaJI GCsGC Cuts at: 976 979 1603 1606 4537 4540 5564 5701 5819 Size: 3 624 3 2931 3 1024 137 118 Cuts at: 5819 5974 7058 7180 7409 976 Size: 155 1084 122 229 1331 Fragments arranged by size: 2931 1331 1084 1024 624 229 155 137 122 118 3 3 3 VspI AT'TA_AT Cuts at: 582 986 6839 7663 582 Size: 404 5853 824 683 Fragments arranged by size: 5853 824 683 404 XbaI T'CTAG_A Cuts at: 1773 1773 Size: 7764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 706 706 Size: 7764 XmnI GAAnn'nnTTC Cuts at: 987 2435 3867 4871 7266 7664 987 Size: 1448 1432 1004 2395 398 1087 Fragments arranged by size: 2395 1448 1432 1087 1004 398 Enzymes that do cut: AatII AccI AceIII AciI AflIII AgeI AluI AlwI Alw21I Alw44I AlwNI ApaBI ApoI AvaII AvrII BamHI BanI BanII BbsI BbvI BccI Bce83I BcefI BcgI BclI BfaI BglI BglII BmgI BpmI Bpu1102I BsaI BsaAI BsaBI BsaHI BsaJI BsaWI BsaXI BsbI BscGI BseRI BsgI BsiI BsiEI BslI BsmI BsmAI BsmBI BsmFI BsoFI Bsp24I Bsp1286I BspEI BspGI BspLU11I BspMI BsrI BsrBI BsrDI BsrFI BsrGI Bst1107I BstXI BstYI Bsu36I Cac8I CjeI CjePI ClaI CviJI CviRI DdeI DpnI DraI DraIII DrdI DrdII DsaI EaeI EagI Eam1105I EarI EciI Eco47III Eco57I EcoNI EcoO109I EcoRI EcoRII EcoRV FauI FokI FspI GdiII HaeI HaeII HaeIII HgaI HgiEII HhaI Hin4I HincII HindIII HinfI HpaI HphI KpnI MaeII MaeIII MboII MmeI MnlI MscI MseI MslI MspI MspA1I MunI MwoI NarI NciI NcoI NdeI NgoAIV NheI NlaIII NlaIV NotI NsiI NspI NspV Pfl1108I PflMI PleI PmeI Psp5II Psp1406I PstI PvuI PvuII RcaI RleAI RsaI SacI SalI SapI Sau96I Sau3AI ScaI ScrFI SfaNI SfcI SgrAI SnaBI SphI SspI StuI StyI SunI TaqI TaqII TfiI ThaI TseI Tsp45I Tsp509I Tth111I Tth111II UbaJI VspI XbaI XcmI XmnI Enzymes that do not cut: AflII ApaI AscI AvaI BaeI Bpu10I BssHII BstEII FseI MluI NruI PacI PmlI PshAI RsrII SacII SexAI SfiI SgfI SmaI SpeI SrfI Sse8387I SwaI XhoI