File: pSecTagA.seq 24-0245 Mode: Normal 1 - 5166 Table: DNASIS# Style: Circular Indication Mode: Actual Cutting Site Enzyme Name Sequence Count Cutting Position AatI AGG!CCT 1 2206 AatII GACGT!C 6 376 429 512 698 2382 5166 Acc65I G!GTACC 1 1004 AccI GT!MKAC 5 34 1130 2971 2978 5166 AccII CG!CG 24 121 209 231 311 969 988 1453 1477 1497 1873 2372 2374 2376 2489 2541 2661 2805 3198 3200 3398 3979 4309 4802 5134 AccIII T!CCGGA 0 AciI G!CGG 25 621 654 779 969 1065 1390 1414 1463 1477 1480 2390 2489 2541 2644 2805 3200 3210 3277 3284 3305 3801 3945 4589 4756 5085 AclI A!ACGTT 2 4469 4842 AcsI R!AATTY 4 128 1039 1864 2890 AcyI GR!CGYC 9 373 426 509 695 2379 2598 2781 4781 5163 AfaI GT!AC 13 45 215 470 495 550 583 634 791 934 994 1006 2558 4724 AflI G!GWCC 6 2403 2458 2491 2497 4382 4604 AflII C!TTAAG 0 AflIII A!CRYGT 4 229 2266 2710 3351 AgeI A!CCGGT 0 AhaI CC!SGG 12 2229 2230 2425 2426 2462 2594 2605 2666 2777 3731 4427 4778 AhaII GR!CGYC 9 373 426 509 695 2379 2598 2781 4781 5163 AhaIII TTT!AAA 4 1159 4110 4129 4821 AluI AG!CT 25 136 817 892 1000 1012 1427 1594 1851 2171 2225 2235 2554 2855 2988 2994 3016 3111 3175 3293 3519 3655 3912 4433 4533 4596 Alw44I G!TGCAC 3 2681 3665 4911 AlwI GGATCNNNN! 8 13 1024 1121 2261 2817 4000 4097 4877 AlwI !NNNNNGATCC 8 16 1011 2788 3912 3998 4096 4560 4881 AlwNI CAGNNN!CTG 2 81 3767 AocI CC!TNAGG 0 AocII GDGCH!C 10 819 1014 1088 1399 1435 2367 2685 3669 4830 4915 Aor51HI AGC!GCT 0 AosI TGC!GCA 1 4466 AosII GR!CGYC 9 373 426 509 695 2379 2598 2781 4781 5163 ApaI GGGCC!C 1 1088 ApaLI G!TGCAC 3 2681 3665 4911 ApoI R!AATTY 4 128 1039 1864 2890 ApyI CC!WGG 17 341 534 952 983 1240 1921 1976 1993 2495 2501 2522 2527 2546 3091 3379 3500 3513 AquI C!YCGRG 4 1072 2228 2414 2424 AscI GG!CGCGCC 1 986 AseI AT!TAAT 7 258 863 1878 2278 3122 3181 4416 AsnI AT!TAAT 7 258 863 1878 2278 3122 3181 4416 Asp700I GAANN!NNTTC 2 1879 4843 Asp718I G!GTACC 1 1004 AspHI GWGCW!C 7 819 1014 2367 2685 3669 4830 4915 AspI GACN!NNGTC 0 AsuI G!GNCC 16 218 334 527 971 1084 1085 1687 2403 2458 2491 2497 2606 4286 4365 4382 4604 AsuII TT!CGAA 0 AvaI C!YCGRG 4 1072 2228 2414 2424 AvaII G!GWCC 6 2403 2458 2491 2497 4382 4604 AvaIII ATGCA!T 2 1957 2029 AviII TGC!GCA 1 4466 AvrI !CYCGRG 4 1071 2227 2413 2423 AvrII C!CTAGG 1 2207 AxyI CC!TNAGG 0 BalI TGG!CCA 1 2339 BamHI G!GATCC 1 1016 BanI G!GYRCC 7 716 1004 1246 1640 2506 3095 4192 BanII GRGCY!C 5 819 1014 1088 1399 1435 BanIII AT!CGAT 0 BbeI GGCGC!C 0 BbiII/AcyI GR!CGYC 9 373 426 509 695 2379 2598 2781 4781 5163 BbrPI CAC!GTG 2 2267 2713 BbsI !GTCTTC 0 BbsI !GAAGAC 1 1362 BbvI GCAGCNNNNNNNN! 7 1511 2644 2864 3770 3773 3979 4673 BbvI !NNNNNNNNNNNNGCTGC 7 188 925 3162 3243 3261 3680 4282 BbvII GAAGACNN! 1 1370 BbvII !NNNNNNGTCTTC 0 BcefI ACGGCNNNNNNNNNNNN! 2 1654 3853 BcefI !NNNNNNNNNNNNNGCCGT 4 976 1112 2340 2621 BcgI !GCANNNNNNTCG 0 BcgI !CGANNNNNNTGC 1 4759 BclI T!GATCA 2 1168 2258 BcnI CC!SGG 12 2229 2230 2425 2426 2462 2594 2605 2666 2777 3731 4427 4778 BfaI C!TAG 15 251 830 897 1023 1079 1190 1436 1528 2208 2925 2985 2989 3846 4099 4434 BfrI C!TTAAG 0 BglI GCCNNNN!NGGC 7 341 463 534 978 983 2641 4364 BglII A!GATCT 1 13 BinI !NNNNNGATCC 8 16 1011 2788 3912 3998 4096 4560 4881 BinI GGATCNNNN! 8 13 1024 1121 2261 2817 4000 4097 4877 BlnI C!CTAGG 1 2207 BmyI GDGCH!C 10 819 1014 1088 1399 1435 2367 2685 3669 4830 4915 BpmI !CTCCAG 2 2797 4328 BpmI !CTGGAG 1 2818 Bpu1102I GC!TNAGC 0 BsaAI YAC!GTR 4 591 1681 2267 2713 BsaBI GATNN!NNATC 0 BsaHI GR!CGYC 9 373 426 509 695 2379 2598 2781 4781 5163 BsaI GGTCTCN! 0 BsaI !NNNNNGAGACC 2 877 4305 BsaJI C!CNNGG 17 611 904 1238 1919 1991 2114 2149 2207 2228 2334 2424 2520 2526 2636 2694 3090 3511 BsaWI W!CCGGW 4 2359 3557 3704 4535 BseAI T!CCGGA 0 BsePI !GCGCGC 2 985 2371 BsgI !GTGCAG 0 BsgI !CTGCAC 0 BsiEI CGRY!CG 8 1069 2394 2410 2670 3267 3691 4614 4763 BsiHKAI GWGCW!C 7 819 1014 2367 2685 3669 4830 4915 BsiWI C!GTACG 1 992 BsiYI CCNNNNN!NNGG 15 300 929 1462 1788 2115 2500 2526 2527 2532 2806 3199 3373 3391 3557 3836 BslI CCNNNNN!NNGG 15 300 929 1462 1788 2115 2500 2526 2527 2532 2806 3199 3373 3391 3557 3836 BsmAI !GTCTC 2 679 5075 BsmAI !GAGAC 3 882 908 4310 BsmFI GGGACNNNNNNNNNNNNNN! 7 430 581 749 2445 2481 2613 2796 BsmFI GGGACNNNNNNNNNN! 7 426 577 745 2441 2477 2609 2792 BsmI GAATGCN! 0 BsmI G!CATTC 1 2920 Bsp120I G!GGCCC 1 1084 Bsp1286I GDGCH!C 10 819 1014 1088 1399 1435 2367 2685 3669 4830 4915 BspDI AT!CGAT 0 BspEI T!CCGGA 0 BspHI T!CATGA 2 4071 5079 BspMI ACCTGCNNNN! 0 BspMI !NNNNNNNNGCAGGT 0 BspMII T!CCGGA 0 BsrB1 GAG!CGG 3 2390 3284 5085 BsrDI GCAATG!NN 1 4303 BsrDI GCAATGNN! 1 4305 BsrFI R!CCGGY 8 977 1576 2409 2450 2608 2669 2783 4324 BsrGI T!GTACA 0 BsrI ACTGGN! 6 853 965 1770 3759 3772 4720 BsrI C!CAGT 11 75 546 1028 2092 2349 3151 3884 4290 4408 4451 4890 BssHII G!CGCGC 2 986 2372 BssSI C!TCGTG 2 3524 4908 BssSI C!TCGTG 2 3524 4908 Bst1107I GTA!TAC 1 2972 BstBI TT!CGAA 0 BstEII G!GTNACC 0 BstI G!GATCC 1 1016 BstNI CC!WGG 17 341 534 952 983 1240 1921 1976 1993 2495 2501 2522 2527 2546 3091 3379 3500 3513 BstPI G!GTNACC 0 BstUI CG!CG 24 121 209 231 311 969 988 1453 1477 1497 1873 2372 2374 2376 2489 2541 2661 2805 3198 3200 3398 3979 4309 4802 5134 BstXI CCANNNNN!NTGG 2 1035 1061 BstYI R!GATCY 11 13 1016 1113 2253 2809 3992 4003 4089 4101 4869 4886 Bsu36I CC!TNAGG 0 CbiI TT!CGAA 0 CcrI C!TCGAG 1 1072 CelII GC!TNAGC 0 CfoI GCG!C 30 121 201 988 990 1126 1455 1468 1477 1499 1525 1533 2374 2376 2489 2541 2661 2805 3135 3200 3228 3261 3531 3598 3698 3872 3981 4374 4467 4804 5136 Cfr10I R!CCGGY 8 977 1576 2409 2450 2608 2669 2783 4324 Cfr13I G!GNCC 16 218 334 527 971 1084 1085 1687 2403 2458 2491 2497 2606 4286 4365 4382 4604 Cfr9I C!CCGGG 2 2228 2424 CfrI Y!GGCCR 8 979 1066 2337 2610 2667 2691 3190 4632 ClaI AT!CGAT 0 CpoI CG!GSCCG 1 2606 Csp45I TT!CGAA 0 Csp6I G!TAC 13 44 214 469 494 549 582 633 790 933 993 1005 2557 4723 CspI CG!GWCCG 0 CvnI CC!TNAGG 0 DdeI C!TNAG 11 40 110 181 1104 1300 1409 2167 3626 4035 4201 4741 DpnI GA!TC 24 7 15 23 1018 1115 1170 2255 2260 2624 2795 2811 3919 3994 4005 4013 4091 4103 4208 4549 4567 4613 4871 4888 4924 DpnII !GATC 24 5 13 21 1016 1113 1168 2253 2258 2622 2793 2809 3917 3992 4003 4011 4089 4101 4206 4547 4565 4611 4869 4886 4922 DraI TTT!AAA 4 1159 4110 4129 4821 DraII RG!GNCCY 1 1084 DraIII CACNNN!GTG 2 1684 2689 DrdI GACNNNN!NNGTC 2 1728 3459 DsaI C!CRYGG 5 611 904 2114 2334 2636 DsaV !CCNGG 29 339 532 950 981 1238 1919 1974 1991 2227 2228 2423 2424 2460 2493 2499 2520 2525 2544 2592 2603 2664 2775 3089 3377 3498 3511 3729 4425 4776 EaeI Y!GGCCR 8 979 1066 2337 2610 2667 2691 3190 4632 EagI C!GGCCG 2 1066 2667 Eam1105I GACNNN!NNGTC 1 4244 EarI !CTCTTC 2 3228 5032 EarI !GAAGAG 1 1107 Ecl136II GAG!CTC 2 817 1012 EclXI C!GGCCG 2 1066 2667 Eco105I TAC!GTA 1 591 Eco31I GGTCTCN! 0 Eco31I !NNNNNGAGACC 2 877 4305 Eco47I G!GWCC 6 2403 2458 2491 2497 4382 4604 Eco47III AGC!GCT 0 Eco52I C!GGCCG 2 1066 2667 Eco57I !CTGAAG 1 3877 Eco57I !CTTCAG 1 4925 Eco81I CC!TNAGG 0 EcoNI CCTNN!NNNAGG 0 EcoO109I RG!GNCCY 1 1084 EcoO65I G!GTNACC 0 EcoRI G!AATTC 1 1039 EcoRII !CCWGG 17 339 532 950 981 1238 1919 1974 1991 2493 2499 2520 2525 2544 3089 3377 3498 3511 EcoRV GAT!ATC 1 1051 EcoT14I C!CWWGG 5 611 904 2114 2207 2334 EcoT22I ATGCA!T 2 1957 2029 EcoT38I GRGCY!C 5 819 1014 1088 1399 1435 EheI GGC!GCC 0 Esp3I CGTCTCN! 0 Esp3I !NNNNNGAGACG 0 EspI GC!TNAGC 0 FbaI T!GATCA 2 1168 2258 FinI !GTCCC 3 1915 1987 2051 FinI !GGGAC 7 411 562 730 2426 2462 2594 2777 Fnu4HI GC!NGC 29 62 202 939 970 1066 1069 1464 1478 1500 2155 2542 2633 2739 2853 3176 3257 3275 3278 3396 3551 3694 3759 3762 3968 4296 4635 4662 4757 4986 FokI GGATGNNNNNNNNN! 2 1399 2803 FokI !NNNNNNNNNNNNNCATCC 5 1594 2058 4210 4391 4678 FspI TGC!GCA 1 4466 GdiII !NNNNNYGGCCG 4 1060 2661 2685 4626 GdiII CGGCCRN! 5 985 1072 2616 2673 3196 GsuI !CTCCAG 2 2797 4328 GsuI !CTGGAG 1 2818 HaeI WGG!CCW 6 2206 2339 2525 3366 3377 3829 HaeII RGCGC!Y 5 1127 1526 1534 3229 3599 HaeIII GG!CC 27 220 335 528 972 981 1068 1086 1689 1831 2148 2154 2206 2339 2525 2544 2608 2612 2669 2693 3192 3366 3377 3395 3829 4287 4367 4634 HapII C!CGG 27 978 1577 2229 2360 2385 2410 2425 2451 2461 2510 2593 2604 2609 2666 2670 2776 2784 3069 3558 3705 3731 3921 4325 4359 4426 4536 4778 HgaI GACGCNNNNN! 6 777 975 2606 2789 3462 4040 HgaI !NNNNNNNNNNGCGTC 1 4770 HgiAI GWGCW!C 7 819 1014 2367 2685 3669 4830 4915 HgiEII !ACCNNNNNNGGT 3 1236 2408 3931 HhaI GCG!C 30 121 201 988 990 1126 1455 1468 1477 1499 1525 1533 2374 2376 2489 2541 2661 2805 3135 3200 3228 3261 3531 3598 3698 3872 3981 4374 4467 4804 5136 Hin1I GR!CGYC 9 373 426 509 695 2379 2598 2781 4781 5163 HinP1I G!CGC 30 119 199 986 988 1124 1453 1466 1475 1497 1523 1531 2372 2374 2487 2539 2659 2803 3133 3198 3226 3259 3529 3596 3696 3870 3979 4372 4465 4802 5134 HincII GTY!RAC 7 35 235 1131 2272 2346 2979 1 HindIII A!AGCTT 1 998 HinfI G!ANTC 14 36 174 661 870 1075 1729 1751 2729 2766 3186 3251 3326 3722 4239 HpaI GTT!AAC 0 HpaII C!CGG 27 978 1577 2229 2360 2385 2410 2425 2451 2461 2510 2593 2604 2609 2666 2670 2776 2784 3069 3558 3705 3731 3921 4325 4359 4426 4536 4778 HphI GGTGANNNNNNNN! 6 627 975 1681 2332 4730 4971 HphI !NNNNNNNTCACC 4 2359 4087 4314 4936 ItaI GC!NGC 29 62 202 939 970 1066 1069 1464 1478 1500 2155 2542 2633 2739 2853 3176 3257 3275 3278 3396 3551 3694 3759 3762 3968 4296 4635 4662 4757 4986 KasI G!GCGCC 0 KpnI GGTAC!C 1 1008 Ksp632I CTCTTCN! 2 3235 5039 Ksp632I !NNNNGAAGAG 1 1103 KspI CCGC!GG 0 MaeI C!TAG 15 251 830 897 1023 1079 1190 1436 1528 2208 2925 2985 2989 3846 4099 4434 MaeII A!CGT 18 373 385 426 509 590 695 1570 1680 1723 1735 2266 2379 2469 2712 4054 4470 4843 5163 MaeIII !GTNAC 16 312 399 748 963 1491 1503 2470 2864 3707 3770 3886 4169 4500 4558 4711 4899 MamI GATNN!NNATC 0 MboI !GATC 24 5 13 21 1016 1113 1168 2253 2258 2622 2793 2809 3917 3992 4003 4011 4089 4101 4206 4547 4565 4611 4869 4886 4922 MboII GAAGANNNNNNNN! 4 183 1120 1375 4013 MboII !NNNNNNNTCTTC 7 1542 2818 3222 4084 4839 4917 5026 McrI !CGRYCG 8 1065 2390 2406 2666 3263 3687 4610 4759 MfeI !CAATTG 1 161 MflI R!GATCY 11 13 1016 1113 2253 2809 3992 4003 4089 4101 4869 4886 MluI A!CGCGT 1 229 MluNI TGG!CCA 1 2339 MmeI !TCCRAC 2 3540 3724 MmeI !GTYGGA 3 98 1724 2568 MnlI CCTCNNNNNNN! 12 1185 1227 1654 2168 2174 2611 2807 2992 3250 3533 4411 4617 MnlI !NNNNNNNGAGG 21 95 791 1074 1103 1271 1346 1403 2137 2143 2180 2183 2195 2315 2430 2565 2688 3199 3458 3782 4182 4263 MroI T!CCGGA 0 MscI TGG!CCA 1 2339 MseI T!TAA 22 70 132 258 863 1158 1471 1742 1840 1857 1868 1878 2278 3122 3181 4057 4109 4114 4128 4181 4416 4455 4820 MslI CARNN!NNRTG 9 166 1033 1059 1941 1976 2013 2678 3632 4895 MspI C!CGG 27 978 1577 2229 2360 2385 2410 2425 2451 2461 2510 2593 2604 2609 2666 2670 2776 2784 3069 3558 3705 3731 3921 4325 4359 4426 4536 4778 MstI TGC!GCA 1 4466 MstII CC!TNAGG 0 MunI C!AATTG 1 162 MvaI CC!WGG 17 341 534 952 983 1240 1921 1976 1993 2495 2501 2522 2527 2546 3091 3379 3500 3513 MvnI CG!CG 24 121 209 231 311 969 988 1453 1477 1497 1873 2372 2374 2376 2489 2541 2661 2805 3198 3200 3398 3979 4309 4802 5134 NaeI GCC!GGC 5 979 1578 2610 2671 2785 NarI GG!CGCC 0 NciI CC!SGG 12 2229 2230 2425 2426 2462 2594 2605 2666 2777 3731 4427 4778 NcoI C!CATGG 4 611 904 2114 2334 NdeI CA!TATG 1 485 NdeII !GATC 24 5 13 21 1016 1113 1168 2253 2258 2622 2793 2809 3917 3992 4003 4011 4089 4101 4206 4547 4565 4611 4869 4886 4922 NgoMI G!CCGGC 5 977 1576 2608 2669 2783 NheI G!CTAGC 1 896 NlaIII CATG! 20 171 555 615 908 1381 1955 2027 2118 2338 2617 2818 2966 3009 3355 4075 4566 4576 4654 4690 5083 NlaIV GGN!NCC 20 718 949 956 1006 1018 1086 1248 1621 1642 1925 1997 2508 3097 3383 3422 4194 4288 4329 4540 5130 NotI GC!GGCCGC 1 1066 NruI TCG!CGA 1 209 NsiI ATGCA!T 2 1957 2029 Nsp(7524)I RCATG!Y 4 1381 1955 2027 3355 Nsp(7524)V TT!CGAA 0 NspBII CMG!CKG 6 1166 1427 3175 3693 3938 4879 NspII GDGCH!C 10 819 1014 1088 1399 1435 2367 2685 3669 4830 4915 NspIII C!YCGRG 4 1072 2228 2414 2424 NspIV G!GNCC 16 218 334 527 971 1084 1085 1687 2403 2458 2491 2497 2606 4286 4365 4382 4604 NunII GG!CGCC 0 PacI TTAAT!TAA 0 PaeR71 C!TCGAG 1 1072 PalI GG!CC 27 220 335 528 972 981 1068 1086 1689 1831 2148 2154 2206 2339 2525 2544 2608 2612 2669 2693 3192 3366 3377 3395 3829 4287 4367 4634 PflMI CCANNNN!NTGG 0 PleI GAGTCNNNN! 3 1083 1737 3730 PleI !NNNNNGACTC 6 30 655 864 1745 3245 4233 PmaCI CAC!GTG 2 2267 2713 PmeI GTTT!AAAC 1 1159 PmlI CAC!GTG 2 2267 2713 Ppu10I A!TGCAT 2 1953 2025 PpuMI RG!GWCCY 0 PshAI GACNN!NNGTC 0 Psp1406I AA!CGTT 2 4470 4843 PstI CTGCA!G 1 1048 PvuI CGAT!CG 1 4614 PvuII CAG!CTG 2 1427 3175 RmaI C!TAG 15 251 830 897 1023 1079 1190 1436 1528 2208 2925 2985 2989 3846 4099 4434 RsaI GT!AC 13 45 215 470 495 550 583 634 791 934 994 1006 2558 4724 RsrI G!AATTC 1 1039 RsrII CG!GWCCG 0 SacI GAGCT!C 2 819 1014 SacII CCGC!GG 0 SalI G!TCGAC 4 33 1129 2977 5165 SapI CGAGAAGNNNN! 0 SapI GCTCTTCN! 1 3235 Sau3AI !GATC 24 5 13 21 1016 1113 1168 2253 2258 2622 2793 2809 3917 3992 4003 4011 4089 4101 4206 4547 4565 4611 4869 4886 4922 Sau96I G!GNCC 16 218 334 527 971 1084 1085 1687 2403 2458 2491 2497 2606 4286 4365 4382 4604 SauI CC!TNAGG 0 ScaI AGT!ACT 1 4724 ScrFI CC!NGG 29 341 534 952 983 1240 1921 1976 1993 2229 2230 2425 2426 2462 2495 2501 2522 2527 2546 2594 2605 2666 2777 3091 3379 3500 3513 3731 4427 4778 SduI GDGCH!C 10 819 1014 1088 1399 1435 2367 2685 3669 4830 4915 SecI C!CNNGG 17 611 904 1238 1919 1991 2114 2149 2207 2228 2334 2424 2520 2526 2636 2694 3090 3511 SexI !CTCGAG 1 1071 SfaNI GCATCNNNNN! 8 1297 1616 1964 2036 2892 3448 4500 4940 SfaNI !NNNNNNNNNGATGC 4 48 608 1377 4691 SfcI C!TYRAG 5 1072 3457 3719 3996 4864 SfiI GGCCNNNN!NGGCC 1 978 SfuI TT!CGAA 0 SgrAI CR!CCGGYG 1 2450 SinI G!GWCC 6 2403 2458 2491 2497 4382 4604 SmaI CCC!GGG 2 2230 2426 SnaBI TAC!GTA 1 591 SnaI !GTATAC 1 2969 SnoI G!TGCAC 3 2681 3665 4911 SpeI A!CTAGT 2 250 1022 SphI GCATG!C 3 1381 1955 2027 SplI C!GTACG 1 992 SpoI !CGTACG 1 991 SrfI GCCC!GGGC 0 Sse8387I CCTGCA!GG 0 SspBI T!GTACA 0 SspI AAT!ATT 1 5048 SstI GAGCT!C 2 819 1014 SstII CCGC!GG 0 SstIII !ACGT 18 372 384 425 508 589 694 1569 1679 1722 1734 2265 2378 2468 2711 4053 4469 4842 5162 StuI AGG!CCT 1 2206 StyI C!CWWGG 5 611 904 2114 2207 2334 StySJI !GAGNNNNNNGTRC 0 StySJI !GYACNNNNNNCTC 1 43 SwaI ATTT!AAAT 0 TaqI T!CGA 12 34 857 1073 1130 1177 1646 2394 2727 2978 3451 4895 5166 TaqII GACCGANNNNNNNNNNN! 4 172 2420 2633 4777 TaqII !NNNNNNNNNTCGGTC 3 1779 3253 4592 TaqII CACCCANNNNNNNNNNN! 1 4930 TaqII !NNNNNNNNNTGGGTG 4 423 2518 2524 4947 TfiI G!AWTC 5 174 2729 2766 3186 3326 ThaI CG!CG 24 121 209 231 311 969 988 1453 1477 1497 1873 2372 2374 2376 2489 2541 2661 2805 3198 3200 3398 3979 4309 4802 5134 Tsp45I !GTSAC 5 963 1503 2470 4500 4711 Tsp509I !AATT 20 128 162 269 860 1039 1283 1864 1875 1879 1961 2033 2125 2275 2890 3031 3048 3123 4111 4417 4672 TspEI !AATT 20 128 162 269 860 1039 1283 1864 1875 1879 1961 2033 2125 2275 2890 3031 3048 3123 4111 4417 4672 Tth111I GACN!NNGTC 0 Tth111II CAARCANNNNNNNNNNN! 2 3941 3980 Tth111II !NNNNNNNNNTGYTTG 3 80 1199 3948 TthHB8I T!CGA 12 34 857 1073 1130 1177 1646 2394 2727 2978 3451 4895 5166 Van91I CCANNNN!NTGG 0 VspI AT!TAAT 7 258 863 1878 2278 3122 3181 4416 XbaI T!CTAGA 1 1078 XcmI !CCANNNNNNNNNTGG 0 XcyI C!CCGGG 2 2228 2424 XhoI C!TCGAG 1 1072 XhoII R!GATCY 11 13 1016 1113 2253 2809 3992 4003 4089 4101 4869 4886 XmaI C!CCGGG 2 2228 2424 XmaIII C!GGCCG 2 1066 2667 XmnI GAANN!NNTTC 2 1879 4843 XorII CGAT!CG 1 4614