pTracerª-EF A Sequence Range: 1 to 5933 Enzyme #Cuts Positions AatI 2 846 889 AatII 6 121 2223 2276 2359 2545 3598 AccI 4 121 155 3301 3456 AccIII 1 3074 Acc16I 1 5354 Acc65I 1 1715 Acc113I 1 5612 AccB2I 5 903 1323 1417 4129 4487 AccBSI 6 40 1264 1332 1782 3606 4183 AclNI 1 1733 AcsI 8 249 978 1564 1750 2928 2985 3349 3990 AcyI 10 118 1414 2220 2273 2356 2542 2696 3595 3814 5669 AflII 1 854 AflIII 5 350 1108 1846 3174 4250 AgeI 2 553 1852 AhdI 1 5132 Alw21I 8 1303 1725 2666 3583 3901 4557 5718 5803 Alw26I 10 36 949 968 1169 1513 1683 1840 2532 2687 5193 Alw44I 3 3897 4553 5799 AlwNI 2 202 4655 Ama87I 9 520 622 808 1323 1440 1783 3273 3630 3640 ApaI 2 1092 1799 ApaLI 3 3897 4553 5799 ApoI 8 249 978 1564 1750 2928 2985 3349 3990 AseI 3 2792 4081 5304 AsnI 3 2792 4081 5304 Asp700I 2 774 5731 Asp718I 1 1715 AspEI 1 5132 AspHI 8 1303 1725 2666 3583 3901 4557 5718 5803 AvaI 9 520 622 808 1323 1440 1783 3273 3630 3640 AvaII 10 441 2735 2777 3506 3619 3674 3707 3713 5270 5492 AviII 1 5354 BalI 1 3023 BamHI 2 1727 3372 BanII 7 864 1092 1303 1725 1799 2122 2666 BbeI 1 1417 BbiII 10 118 1414 2220 2273 2356 2542 2696 3595 3814 5669 BbsI 2 2093 2732 BbuI 1 2104 Bbv12I 8 1303 1725 2666 3583 3901 4557 5718 5803 Bbv16II 2 2093 2732 BclI 1 1891 BcoI 9 520 622 808 1323 1440 1783 3273 3630 3640 BfmI 7 787 1292 1682 1755 4504 4695 5373 BfrI 1 854 BglI 9 1246 1422 1539 2188 2310 2381 2752 3857 5252 BglII 2 134 1041 Bme18I 10 441 2735 2777 3506 3619 3674 3707 3713 5270 5492 BpiI 2 2093 2732 BpmI 3 2711 2899 5202 Bpu14I 2 1802 3474 BpuAI 2 2093 2732 BsaI 3 1683 1840 5193 BsaAI 2 795 2438 BsaHI 10 118 1414 2220 2273 2356 2542 2696 3595 3814 5669 BsaMI 1 4020 BsaOI 10 1246 1780 2756 3610 3626 3886 4166 4579 5502 5651 BsaWI 8 478 553 1852 3074 3575 4445 4592 5423 Bse118I 8 553 1175 1852 3625 3666 3824 3885 5212 BseAI 1 3074 BsePI 1 3588 BseRI 2 1819 3927 BsgI 2 671 1030 Bsh1285I 10 1246 1780 2756 3610 3626 3886 4166 4579 5502 5651 BsiEI 10 1246 1780 2756 3610 3626 3886 4166 4579 5502 5651 BsiHKAI 8 1303 1725 2666 3583 3901 4557 5718 5803 BsiMI 1 3074 BsmI 1 4020 BsmBI 1 2687 BsmFI 10 502 1083 2273 2424 2592 2748 2871 3657 3693 3825 BsoBI 9 520 622 808 1323 1440 1783 3273 3630 3640 Bsp13I 1 3074 Bsp19I 3 2458 2848 3018 Bsp68I 1 330 Bsp119I 2 1802 3474 Bsp120I 2 1088 1795 Bsp143II 5 903 1323 1417 4129 4487 Bsp1407I 1 3128 BspCI 1 5502 BspEI 1 3074 BspHI 2 34 4959 BspLU11I 2 1108 4250 BspMI 1 999 BspTI 1 854 BsrBI 6 40 1264 1332 1782 3606 4183 BsrDI 2 5193 5367 BsrFI 8 553 1175 1852 3625 3666 3824 3885 5212 BsrGI 1 3128 BssAI 8 553 1175 1852 3625 3666 3824 3885 5212 BssHII 1 3588 BssSI 4 468 872 4412 5796 BssT1I 4 1559 2458 2848 3018 Bst98I 1 854 Bst1107I 1 3302 BstBI 2 1802 3474 Bst2BI 4 468 872 4412 5796 BstDSI 8 726 1073 1400 2458 2750 2848 3018 3852 BstF5I 6 2115 2699 3557 5111 5292 5579 BstH2I 5 903 1323 1417 4129 4487 BstMCI 10 1246 1780 2756 3610 3626 3886 4166 4579 5502 5651 BstSFI 7 787 1292 1682 1755 4504 4695 5373 BstSNI 1 2438 BstXI 2 1746 1772 BstZI 3 1777 2753 3883 CbiI 2 1802 3474 CciNI 1 1777 Cfr9I 1 3640 Cfr10I 8 553 1175 1852 3625 3666 3824 3885 5212 Cfr42I 3 729 1076 2753 CpoI 1 2777 CspI 1 2777 Csp45I 2 1802 3474 DraI 7 413 976 1882 3242 4998 5017 5709 DraII 5 1088 1125 1364 1795 1796 DraIII 2 1511 3905 DrdI 2 3504 4347 DsaI 8 726 1073 1400 2458 2750 2848 3018 3852 EagI 3 1777 2753 3883 Eam1104I 2 4135 5927 Eam1105I 1 5132 EarI 2 4135 5927 Ecl136II 3 1301 1723 2664 EclHKI 1 5132 EclXI 3 1777 2753 3883 Eco24I 7 864 1092 1303 1725 1799 2122 2666 Eco31I 3 1683 1840 5193 Eco32I 1 1762 Eco47I 10 441 2735 2777 3506 3619 3674 3707 3713 5270 5492 Eco52I 3 1777 2753 3883 Eco57I 4 1544 3202 4787 5799 Eco88I 9 520 622 808 1323 1440 1783 3273 3630 3640 Eco105I 1 2438 Eco130I 4 1559 2458 2848 3018 Eco147I 2 846 889 Eco255I 1 5612 EcoICRI 3 1301 1723 2664 EcoNI 2 564 1292 EcoO109I 5 1088 1125 1364 1795 1796 EcoRI 1 1750 EcoRV 1 1762 EcoT14I 4 1559 2458 2848 3018 EcoT38I 7 864 1092 1303 1725 1799 2122 2666 EheI 1 1415 ErhI 4 1559 2458 2848 3018 Esp3I 1 2687 FauNDI 2 2332 3082 FbaI 1 1891 FokI 6 2122 2686 3564 5098 5279 5566 FriOI 7 864 1092 1303 1725 1799 2122 2666 FseI 2 1179 3828 FspI 1 5354 GsuI 3 2711 2899 5202 HaeII 5 903 1323 1417 4129 4487 HgaI 10 1008 1085 1323 2624 2704 3182 3822 4350 4928 5658 HgiEI 10 441 2735 2777 3506 3619 3674 3707 3713 5270 5492 Hin1I 10 118 1414 2220 2273 2356 2542 2696 3595 3814 5669 HincII 6 122 156 356 2786 3457 3562 HindII 6 122 156 356 2786 3457 3562 HindIII 3 387 1709 2973 Hsp92I 10 118 1414 2220 2273 2356 2542 2696 3595 3814 5669 KasI 1 1413 KpnI 1 1719 Kpn2I 1 3074 KspI 3 729 1076 2753 Ksp22I 1 1891 Ksp632I 2 4135 5927 LspI 2 1802 3474 MfeI 3 283 546 3412 MluI 3 350 1846 3174 MluNI 1 3023 MroI 1 3074 MroNI 3 1175 3824 3885 MscI 1 3023 MslI 4 2463 5384 5543 5902 Msp17I 10 118 1414 2220 2273 2356 2542 2696 3595 3814 5669 MspA1I 7 728 1075 1889 2752 4581 4826 5767 MspCI 1 854 MunI 3 283 546 3412 Mva1269I 1 4020 NaeI 3 1177 3826 3887 NarI 1 1414 NcoI 3 2458 2848 3018 NdeI 2 2332 3082 NgoAIV 3 1175 3824 3885 NgoMI 3 1175 3824 3885 NheI 1 2853 NotI 1 1777 NruI 1 330 NspI 3 1112 2104 4254 NspV 2 1802 3474 NspBII 7 728 1075 1889 2752 4581 4826 5767 PaeI 1 2104 PaeR7I 3 1440 1783 3273 PauI 1 3588 PinAI 2 553 1852 PleI 9 151 1350 1390 1670 1794 2162 2502 4618 5121 Ple19I 1 5502 PmeI 1 1882 Pme55I 2 846 889 PshBI 3 2792 4081 5304 Psp1406I 3 668 5358 5731 PspAI 1 3640 PspALI 1 3642 Psp124BI 3 1303 1725 2666 PspOMI 2 1088 1795 PstI 3 791 1296 1759 PstNHI 1 2853 PvuI 1 5502 RcaI 2 34 4959 RsrII 1 2777 SacI 3 1303 1725 2666 SacII 3 729 1076 2753 SalI 3 120 154 3455 SapI 1 4135 ScaI 1 5612 SexAI 1 3715 SfcI 7 787 1292 1682 1755 4504 4695 5373 Sfr274I 3 1440 1783 3273 Sfr303I 3 729 1076 2753 SfuI 2 1802 3474 SgrAI 1 3666 SinI 10 441 2735 2777 3506 3619 3674 3707 3713 5270 5492 SmaI 1 3642 SnaBI 1 2438 SpeI 1 1733 SphI 1 2104 SseBI 2 846 889 SspI 1 3 SspBI 1 3128 SstI 3 1303 1725 2666 SstII 3 729 1076 2753 StuI 2 846 889 StyI 4 1559 2458 2848 3018 TfiI 9 295 424 798 920 1148 1840 3331 4086 4225 Tsp45I 7 1343 1393 3032 3495 3686 5388 5599 TspRI 10 496 1619 1746 1777 2945 3572 4931 5080 5185 5532 Vha464I 1 854 VneI 3 3897 4553 5799 VspI 3 2792 4081 5304 XbaI 1 1789 XhoI 3 1440 1783 3273 XmaI 1 3640 XmaIII 3 1777 2753 3883 XmaCI 1 3640 XmnI 2 774 5731 The enzymes listed below do not restrict pTracerª-EF A AccB7I AfeI AocI Aor51HI AscI AspI AtsI AvrII AxyI BanIII BbrPI BlnI BlpI Bpu1102I Bsa29I BsaBI BscI Bse8I Bse21I BseCI Bsh1365I BsiWI Bsp106I Bsp1720I BspDI BspXI BsrBRI BstEII BstPI Bsu15I Bsu36I CelII ClaI CvnI Eco47III Eco72I Eco81I Eco91I EcoO65I EcoT22I Esp1396I HpaI MamI Mph1103I NsiI PacI Pfl23II PflMI PmaCI PmlI Ppu10I PpuMI PshAI Psp5II PspEI PspLI PvuII SbfI SfiI SgfI SmiI SplI SrfI Sse8387I SunI SwaI Tth111I Van91I XcmI Zsp2I