pTracerª-EF B Sequence Range: 1 to 5937 Enzyme #Cuts Positions AatI 2 846 889 AatII 6 121 2227 2280 2363 2549 3602 AccI 4 121 155 3305 3460 AccIII 1 3078 Acc16I 1 5358 Acc65I 1 1715 Acc113I 1 5616 AccB2I 5 903 1323 1417 4133 4491 AccBSI 6 40 1264 1332 1782 3610 4187 AclNI 1 1733 AcsI 8 249 978 1564 1750 2932 2989 3353 3994 AcyI 10 118 1414 2224 2277 2360 2546 2700 3599 3818 5673 AflII 1 854 AflIII 5 350 1108 1850 3178 4254 AgeI 2 553 1856 AhdI 1 5136 Alw21I 8 1303 1725 2670 3587 3905 4561 5722 5807 Alw26I 10 36 949 968 1169 1513 1683 1844 2536 2691 5197 Alw44I 3 3901 4557 5803 AlwNI 2 202 4659 Ama87I 9 520 622 808 1323 1440 1783 3277 3634 3644 ApaI 2 1092 1799 ApaLI 3 3901 4557 5803 ApoI 8 249 978 1564 1750 2932 2989 3353 3994 AseI 3 2796 4085 5308 AsnI 3 2796 4085 5308 Asp700I 2 774 5735 Asp718I 1 1715 AspEI 1 5136 AspHI 8 1303 1725 2670 3587 3905 4561 5722 5807 AvaI 9 520 622 808 1323 1440 1783 3277 3634 3644 AvaII 10 441 2739 2781 3510 3623 3678 3711 3717 5274 5496 AviII 1 5358 BalI 1 3027 BamHI 2 1727 3376 BanII 7 864 1092 1303 1725 1799 2126 2670 BbeI 1 1417 BbiII 10 118 1414 2224 2277 2360 2546 2700 3599 3818 5673 BbsI 2 2097 2736 BbuI 1 2108 Bbv12I 8 1303 1725 2670 3587 3905 4561 5722 5807 Bbv16II 2 2097 2736 BclI 1 1895 BcoI 9 520 622 808 1323 1440 1783 3277 3634 3644 BfmI 7 787 1292 1682 1755 4508 4699 5377 BfrI 1 854 BglI 9 1246 1422 1539 2192 2314 2385 2756 3861 5256 BglII 2 134 1041 Bme18I 10 441 2739 2781 3510 3623 3678 3711 3717 5274 5496 BpiI 2 2097 2736 BpmI 3 2715 2903 5206 Bpu14I 2 1806 3478 BpuAI 2 2097 2736 BsaI 3 1683 1844 5197 BsaAI 2 795 2442 BsaHI 10 118 1414 2224 2277 2360 2546 2700 3599 3818 5673 BsaMI 1 4024 BsaOI 10 1246 1780 2760 3614 3630 3890 4170 4583 5506 5655 BsaWI 8 478 553 1856 3078 3579 4449 4596 5427 Bse118I 8 553 1175 1856 3629 3670 3828 3889 5216 BseAI 1 3078 BsePI 1 3592 BseRI 2 1823 3931 BsgI 2 671 1030 Bsh1285I 10 1246 1780 2760 3614 3630 3890 4170 4583 5506 5655 BsiEI 10 1246 1780 2760 3614 3630 3890 4170 4583 5506 5655 BsiHKAI 8 1303 1725 2670 3587 3905 4561 5722 5807 BsiMI 1 3078 BsmI 1 4024 BsmBI 1 2691 BsmFI 10 502 1083 2277 2428 2596 2752 2875 3661 3697 3829 BsoBI 9 520 622 808 1323 1440 1783 3277 3634 3644 Bsp13I 1 3078 Bsp19I 3 2462 2852 3022 Bsp68I 1 330 Bsp119I 2 1806 3478 Bsp120I 2 1088 1795 Bsp143II 5 903 1323 1417 4133 4491 Bsp1407I 1 3132 BspCI 1 5506 BspEI 1 3078 BspHI 2 34 4963 BspLU11I 2 1108 4254 BspMI 1 999 BspTI 1 854 BsrBI 6 40 1264 1332 1782 3610 4187 BsrDI 2 5197 5371 BsrFI 8 553 1175 1856 3629 3670 3828 3889 5216 BsrGI 1 3132 BssAI 8 553 1175 1856 3629 3670 3828 3889 5216 BssHII 1 3592 BssSI 4 468 872 4416 5800 BssT1I 4 1559 2462 2852 3022 Bst98I 1 854 Bst1107I 1 3306 BstBI 2 1806 3478 Bst2BI 4 468 872 4416 5800 BstDSI 9 726 1073 1400 1799 2462 2754 2852 3022 3856 BstF5I 6 2119 2703 3561 5115 5296 5583 BstH2I 5 903 1323 1417 4133 4491 BstMCI 10 1246 1780 2760 3614 3630 3890 4170 4583 5506 5655 BstSFI 7 787 1292 1682 1755 4508 4699 5377 BstSNI 1 2442 BstXI 2 1746 1772 BstZI 3 1777 2757 3887 CbiI 2 1806 3478 CciNI 1 1777 Cfr9I 1 3644 Cfr10I 8 553 1175 1856 3629 3670 3828 3889 5216 Cfr42I 4 729 1076 1802 2757 CpoI 1 2781 CspI 1 2781 Csp45I 2 1806 3478 DraI 7 413 976 1886 3246 5002 5021 5713 DraII 4 1088 1125 1364 1795 DraIII 2 1511 3909 DrdI 2 3508 4351 DsaI 9 726 1073 1400 1799 2462 2754 2852 3022 3856 EagI 3 1777 2757 3887 Eam1104I 2 4139 5931 Eam1105I 1 5136 EarI 2 4139 5931 Ecl136II 3 1301 1723 2668 EclHKI 1 5136 EclXI 3 1777 2757 3887 Eco24I 7 864 1092 1303 1725 1799 2126 2670 Eco31I 3 1683 1844 5197 Eco32I 1 1762 Eco47I 10 441 2739 2781 3510 3623 3678 3711 3717 5274 5496 Eco52I 3 1777 2757 3887 Eco57I 4 1544 3206 4791 5803 Eco88I 9 520 622 808 1323 1440 1783 3277 3634 3644 Eco105I 1 2442 Eco130I 4 1559 2462 2852 3022 Eco147I 2 846 889 Eco255I 1 5616 EcoICRI 3 1301 1723 2668 EcoNI 2 564 1292 EcoO109I 4 1088 1125 1364 1795 EcoRI 1 1750 EcoRV 1 1762 EcoT14I 4 1559 2462 2852 3022 EcoT38I 7 864 1092 1303 1725 1799 2126 2670 EheI 1 1415 ErhI 4 1559 2462 2852 3022 Esp3I 1 2691 FauNDI 2 2336 3086 FbaI 1 1895 FokI 6 2126 2690 3568 5102 5283 5570 FriOI 7 864 1092 1303 1725 1799 2126 2670 FseI 2 1179 3832 FspI 1 5358 GsuI 3 2715 2903 5206 HaeII 5 903 1323 1417 4133 4491 HgaI 10 1008 1085 1323 2628 2708 3186 3826 4354 4932 5662 HgiEI 10 441 2739 2781 3510 3623 3678 3711 3717 5274 5496 Hin1I 10 118 1414 2224 2277 2360 2546 2700 3599 3818 5673 HincII 6 122 156 356 2790 3461 3566 HindII 6 122 156 356 2790 3461 3566 HindIII 3 387 1709 2977 Hsp92I 10 118 1414 2224 2277 2360 2546 2700 3599 3818 5673 KasI 1 1413 KpnI 1 1719 Kpn2I 1 3078 KspI 4 729 1076 1802 2757 Ksp22I 1 1895 Ksp632I 2 4139 5931 LspI 2 1806 3478 MfeI 3 283 546 3416 MluI 3 350 1850 3178 MluNI 1 3027 MroI 1 3078 MroNI 3 1175 3828 3889 MscI 1 3027 MslI 4 2467 5388 5547 5906 Msp17I 10 118 1414 2224 2277 2360 2546 2700 3599 3818 5673 MspA1I 8 728 1075 1801 1893 2756 4585 4830 5771 MspCI 1 854 MunI 3 283 546 3416 Mva1269I 1 4024 NaeI 3 1177 3830 3891 NarI 1 1414 NcoI 3 2462 2852 3022 NdeI 2 2336 3086 NgoAIV 3 1175 3828 3889 NgoMI 3 1175 3828 3889 NheI 1 2857 NotI 1 1777 NruI 1 330 NspI 3 1112 2108 4258 NspV 2 1806 3478 NspBII 8 728 1075 1801 1893 2756 4585 4830 5771 PaeI 1 2108 PaeR7I 3 1440 1783 3277 PauI 1 3592 PinAI 2 553 1856 PleI 9 151 1350 1390 1670 1794 2166 2506 4622 5125 Ple19I 1 5506 PmeI 1 1886 Pme55I 2 846 889 PshBI 3 2796 4085 5308 Psp1406I 3 668 5362 5735 PspAI 1 3644 PspALI 1 3646 Psp124BI 3 1303 1725 2670 PspOMI 2 1088 1795 PstI 3 791 1296 1759 PstNHI 1 2857 PvuI 1 5506 RcaI 2 34 4963 RsrII 1 2781 SacI 3 1303 1725 2670 SacII 4 729 1076 1802 2757 SalI 3 120 154 3459 SapI 1 4139 ScaI 1 5616 SexAI 1 3719 SfcI 7 787 1292 1682 1755 4508 4699 5377 Sfr274I 3 1440 1783 3277 Sfr303I 4 729 1076 1802 2757 SfuI 2 1806 3478 SgrAI 1 3670 SinI 10 441 2739 2781 3510 3623 3678 3711 3717 5274 5496 SmaI 1 3646 SnaBI 1 2442 SpeI 1 1733 SphI 1 2108 SseBI 2 846 889 SspI 1 3 SspBI 1 3132 SstI 3 1303 1725 2670 SstII 4 729 1076 1802 2757 StuI 2 846 889 StyI 4 1559 2462 2852 3022 TfiI 9 295 424 798 920 1148 1844 3335 4090 4229 Tsp45I 7 1343 1393 3036 3499 3690 5392 5603 TspRI 10 496 1619 1746 1777 2949 3576 4935 5084 5189 5536 Vha464I 1 854 VneI 3 3901 4557 5803 VspI 3 2796 4085 5308 XbaI 1 1789 XhoI 3 1440 1783 3277 XmaI 1 3644 XmaIII 3 1777 2757 3887 XmaCI 1 3644 XmnI 2 774 5735 The enzymes listed below do not restrict pTracerª-EF B AccB7I AfeI AocI Aor51HI AscI AspI AtsI AvrII AxyI BanIII BbrPI BlnI BlpI Bpu1102I Bsa29I BsaBI BscI Bse8I Bse21I BseCI Bsh1365I BsiWI Bsp106I Bsp1720I BspDI BspXI BsrBRI BstEII BstPI Bsu15I Bsu36I CelII ClaI CvnI Eco47III Eco72I Eco81I Eco91I EcoO65I EcoT22I Esp1396I HpaI MamI Mph1103I NsiI PacI Pfl23II PflMI PmaCI PmlI Ppu10I PpuMI PshAI Psp5II PspEI PspLI PvuII SbfI SfiI SgfI SmiI SplI SrfI Sse8387I SunI SwaI Tth111I Van91I XcmI Zsp2I