pTracerª-EF C Sequence Range: 1 to 5928 Enzyme #Cuts Positions AatI 2 846 889 AatII 6 121 2219 2272 2355 2541 3594 AccI 4 121 155 3297 3452 AccIII 1 3070 Acc16I 1 5349 Acc65I 1 1715 Acc113I 1 5607 AccB2I 5 903 1323 1417 4125 4482 AccBSI 6 40 1264 1332 1782 3602 4178 AclNI 1 1733 AcsI 8 249 978 1564 1750 2924 2981 3345 3986 AcyI 10 118 1414 2216 2269 2352 2538 2692 3591 3810 5664 AflII 1 854 AflIII 5 350 1108 1842 3170 4245 AgeI 2 553 1848 AhdI 1 5127 Alw21I 8 1303 1725 2662 3579 3897 4552 5713 5798 Alw26I 10 36 949 968 1169 1513 1683 1836 2528 2683 5188 Alw44I 3 3893 4548 5794 AlwNI 2 202 4650 Ama87I 9 520 622 808 1323 1440 1783 3269 3626 3636 ApaI 1 1092 ApaLI 3 3893 4548 5794 ApoI 8 249 978 1564 1750 2924 2981 3345 3986 AseI 3 2788 4077 5299 AsnI 3 2788 4077 5299 Asp700I 2 774 5726 Asp718I 1 1715 AspEI 1 5127 AspHI 8 1303 1725 2662 3579 3897 4552 5713 5798 AvaI 9 520 622 808 1323 1440 1783 3269 3626 3636 AvaII 10 441 2731 2773 3502 3615 3670 3703 3709 5265 5487 AviII 1 5349 BalI 1 3019 BamHI 2 1727 3368 BanII 6 864 1092 1303 1725 2118 2662 BbeI 1 1417 BbiII 10 118 1414 2216 2269 2352 2538 2692 3591 3810 5664 BbsI 2 2089 2728 BbuI 1 2100 Bbv12I 8 1303 1725 2662 3579 3897 4552 5713 5798 Bbv16II 2 2089 2728 BclI 1 1887 BcoI 9 520 622 808 1323 1440 1783 3269 3626 3636 BfmI 7 787 1292 1682 1755 4499 4690 5368 BfrI 1 854 BglI 9 1246 1422 1539 2184 2306 2377 2748 3853 5247 BglII 2 134 1041 Bme18I 10 441 2731 2773 3502 3615 3670 3703 3709 5265 5487 BpiI 2 2089 2728 BpmI 3 2707 2895 5197 Bpu14I 2 1798 3470 BpuAI 2 2089 2728 BsaI 3 1683 1836 5188 BsaAI 2 795 2434 BsaHI 10 118 1414 2216 2269 2352 2538 2692 3591 3810 5664 BsaMI 1 4016 BsaOI 10 1246 1780 2752 3606 3622 3882 4161 4574 5497 5646 BsaWI 8 478 553 1848 3070 3571 4440 4587 5418 Bse118I 8 553 1175 1848 3621 3662 3820 3881 5207 BseAI 1 3070 BsePI 1 3584 BseRI 2 1815 3923 BsgI 2 671 1030 Bsh1285I 10 1246 1780 2752 3606 3622 3882 4161 4574 5497 5646 BsiEI 10 1246 1780 2752 3606 3622 3882 4161 4574 5497 5646 BsiHKAI 8 1303 1725 2662 3579 3897 4552 5713 5798 BsiMI 1 3070 BsmI 1 4016 BsmBI 1 2683 BsmFI 10 502 1083 2269 2420 2588 2744 2867 3653 3689 3821 BsoBI 9 520 622 808 1323 1440 1783 3269 3626 3636 Bsp13I 1 3070 Bsp19I 3 2454 2844 3014 Bsp68I 1 330 Bsp119I 2 1798 3470 Bsp120I 1 1088 Bsp143II 5 903 1323 1417 4125 4482 Bsp1407I 1 3124 BspCI 1 5497 BspEI 1 3070 BspHI 2 34 4954 BspLU11I 2 1108 4245 BspMI 1 999 BspTI 1 854 BsrBI 6 40 1264 1332 1782 3602 4178 BsrDI 2 5188 5362 BsrFI 8 553 1175 1848 3621 3662 3820 3881 5207 BsrGI 1 3124 BssAI 8 553 1175 1848 3621 3662 3820 3881 5207 BssHII 1 3584 BssSI 4 468 872 4407 5791 BssT1I 4 1559 2454 2844 3014 Bst98I 1 854 Bst1107I 1 3298 BstBI 2 1798 3470 Bst2BI 4 468 872 4407 5791 BstDSI 8 726 1073 1400 2454 2746 2844 3014 3848 BstEII 1 1788 BstF5I 6 2111 2695 3553 5106 5287 5574 BstH2I 5 903 1323 1417 4125 4482 BstMCI 10 1246 1780 2752 3606 3622 3882 4161 4574 5497 5646 BstPI 1 1788 BstSFI 7 787 1292 1682 1755 4499 4690 5368 BstSNI 1 2434 BstXI 2 1746 1772 BstZI 3 1777 2749 3879 CbiI 2 1798 3470 CciNI 1 1777 Cfr9I 1 3636 Cfr10I 8 553 1175 1848 3621 3662 3820 3881 5207 Cfr42I 3 729 1076 2749 CpoI 1 2773 CspI 1 2773 Csp45I 2 1798 3470 DraI 7 413 976 1878 3238 4993 5012 5704 DraII 3 1088 1125 1364 DraIII 2 1511 3901 DrdI 2 3500 4342 DsaI 8 726 1073 1400 2454 2746 2844 3014 3848 EagI 3 1777 2749 3879 Eam1104I 2 4131 5922 Eam1105I 1 5127 EarI 2 4131 5922 Ecl136II 3 1301 1723 2660 EclHKI 1 5127 EclXI 3 1777 2749 3879 Eco24I 6 864 1092 1303 1725 2118 2662 Eco31I 3 1683 1836 5188 Eco32I 1 1762 Eco47I 10 441 2731 2773 3502 3615 3670 3703 3709 5265 5487 Eco52I 3 1777 2749 3879 Eco57I 4 1544 3198 4782 5794 Eco88I 9 520 622 808 1323 1440 1783 3269 3626 3636 Eco91I 1 1788 Eco105I 1 2434 Eco130I 4 1559 2454 2844 3014 Eco147I 2 846 889 Eco255I 1 5607 EcoICRI 3 1301 1723 2660 EcoNI 2 564 1292 EcoO65I 1 1788 EcoO109I 3 1088 1125 1364 EcoRI 1 1750 EcoRV 1 1762 EcoT14I 4 1559 2454 2844 3014 EcoT38I 6 864 1092 1303 1725 2118 2662 EheI 1 1415 ErhI 4 1559 2454 2844 3014 Esp3I 1 2683 FauNDI 2 2328 3078 FbaI 1 1887 FokI 6 2118 2682 3560 5093 5274 5561 FriOI 6 864 1092 1303 1725 2118 2662 FseI 2 1179 3824 FspI 1 5349 GsuI 3 2707 2895 5197 HaeII 5 903 1323 1417 4125 4482 HgaI 10 1008 1085 1323 2620 2700 3178 3818 4345 4923 5653 HgiEI 10 441 2731 2773 3502 3615 3670 3703 3709 5265 5487 Hin1I 10 118 1414 2216 2269 2352 2538 2692 3591 3810 5664 HincII 6 122 156 356 2782 3453 3558 HindII 6 122 156 356 2782 3453 3558 HindIII 3 387 1709 2969 Hsp92I 10 118 1414 2216 2269 2352 2538 2692 3591 3810 5664 KasI 1 1413 KpnI 1 1719 Kpn2I 1 3070 KspI 3 729 1076 2749 Ksp22I 1 1887 Ksp632I 2 4131 5922 LspI 2 1798 3470 MfeI 3 283 546 3408 MluI 3 350 1842 3170 MluNI 1 3019 MroI 1 3070 MroNI 3 1175 3820 3881 MscI 1 3019 MslI 4 2459 5379 5538 5897 Msp17I 10 118 1414 2216 2269 2352 2538 2692 3591 3810 5664 MspA1I 7 728 1075 1885 2748 4576 4821 5762 MspCI 1 854 MunI 3 283 546 3408 Mva1269I 1 4016 NaeI 3 1177 3822 3883 NarI 1 1414 NcoI 3 2454 2844 3014 NdeI 2 2328 3078 NgoAIV 3 1175 3820 3881 NgoMI 3 1175 3820 3881 NheI 1 2849 NotI 1 1777 NruI 1 330 NspI 3 1112 2100 4249 NspV 2 1798 3470 NspBII 7 728 1075 1885 2748 4576 4821 5762 PaeI 1 2100 PaeR7I 3 1440 1783 3269 PauI 1 3584 PinAI 2 553 1848 PleI 8 151 1350 1390 1670 2158 2498 4613 5116 Ple19I 1 5497 PmeI 1 1878 Pme55I 2 846 889 PshBI 3 2788 4077 5299 Psp1406I 3 668 5353 5726 PspAI 1 3636 PspALI 1 3638 Psp124BI 3 1303 1725 2662 PspEI 1 1788 PspOMI 1 1088 PstI 3 791 1296 1759 PstNHI 1 2849 PvuI 1 5497 RcaI 2 34 4954 RsrII 1 2773 SacI 3 1303 1725 2662 SacII 3 729 1076 2749 SalI 3 120 154 3451 SapI 1 4131 ScaI 1 5607 SexAI 1 3711 SfcI 7 787 1292 1682 1755 4499 4690 5368 Sfr274I 3 1440 1783 3269 Sfr303I 3 729 1076 2749 SfuI 2 1798 3470 SgrAI 1 3662 SinI 10 441 2731 2773 3502 3615 3670 3703 3709 5265 5487 SmaI 1 3638 SnaBI 1 2434 SpeI 1 1733 SphI 1 2100 SseBI 2 846 889 SspI 1 3 SspBI 1 3124 SstI 3 1303 1725 2662 SstII 3 729 1076 2749 StuI 2 846 889 StyI 4 1559 2454 2844 3014 TfiI 9 295 424 798 920 1148 1836 3327 4082 4220 Tsp45I 8 1343 1393 1788 3028 3491 3682 5383 5594 TspRI 10 496 1619 1746 1777 2941 3568 4926 5075 5180 5527 Vha464I 1 854 VneI 3 3893 4548 5794 VspI 3 2788 4077 5299 XhoI 3 1440 1783 3269 XmaI 1 3636 XmaIII 3 1777 2749 3879 XmaCI 1 3636 XmnI 2 774 5726 The enzymes listed below do not restrict pTracerª-EF C AccB7I AfeI AocI Aor51HI AscI AspI AtsI AvrII AxyI BanIII BbrPI BlnI BlpI Bpu1102I Bsa29I BsaBI BscI Bse8I Bse21I BseCI Bsh1365I BsiWI Bsp106I Bsp1720I BspDI BspXI BsrBRI Bsu15I Bsu36I CelII ClaI CvnI Eco47III Eco72I Eco81I EcoT22I Esp1396I HpaI MamI Mph1103I NsiI PacI Pfl23II PflMI PmaCI PmlI Ppu10I PpuMI PshAI Psp5II PspLI PvuII SbfI SfiI SgfI SmiI SplI SrfI Sse8387I SunI SwaI Tth111I Van91I XbaI XcmI Zsp2I