pTracerª-EF/lacZ Sequence Range: 1 to 8980 Enzyme #Cuts Positions AatI 2 846 889 AatII 7 121 2393 5271 5324 5407 5593 6646 AccI 5 121 155 4534 6349 6504 AccIII 1 6122 Acc16I 2 1912 8401 Acc65I 2 1715 1950 Acc113I 1 8659 AccB7I 2 3015 3381 AccBSI 9 40 1264 1332 2375 4273 4437 4834 6654 7230 AclNI 1 1734 AcsI 10 249 978 1564 1751 2266 4774 5976 6033 6397 7038 AfeI 1 3605 AflII 1 854 AflIII 9 350 1108 3072 3852 4277 4529 4894 6222 7297 AgeI 2 553 4900 AhdI 1 8179 Alw44I 5 4115 4230 6945 7600 8846 AlwNI 5 202 3480 3905 4656 7702 AocI 1 1994 Aor51HI 1 3605 ApaI 1 1092 ApaLI 5 4115 4230 6945 7600 8846 ApoI 10 249 978 1564 1751 2266 4774 5976 6033 6397 7038 AseI 3 5840 7129 8351 AsnI 3 5840 7129 8351 Asp700I 2 774 8778 Asp718I 2 1715 1950 AspEI 1 8179 AviII 2 1912 8401 AxyI 1 1994 BalI 1 6071 BamHI 2 1728 6420 BanII 8 864 1092 1303 1725 3710 4759 5170 5714 BanIII 1 2594 BbeI 1 1417 BbsI 4 3287 4539 5141 5780 BbuI 1 5152 Bbv16II 4 3287 4539 5141 5780 BclI 2 3116 4939 BfmI 9 787 1292 1682 1756 2158 4641 7551 7742 8420 BfrI 1 854 BglII 2 134 1041 BlpI 1 4783 BpiI 4 3287 4539 5141 5780 BpmI 7 1999 2497 3359 4773 5759 5947 8249 Bpu14I 2 4850 6522 Bpu1102I 1 4783 BpuAI 4 3287 4539 5141 5780 BsaI 3 1683 4888 8240 Bsa29I 1 2594 BsaAI 2 795 5486 BsaBI 1 3101 BsaMI 1 7068 BsaWI 10 478 553 1947 4325 4900 6122 6623 7492 7639 8470 BscI 1 2594 Bse8I 1 3101 Bse21I 1 1994 Bse118I 10 553 1175 3057 4789 4900 6673 6714 6872 6933 8259 BseAI 1 6122 BseCI 1 2594 BsePI 2 3268 6636 BseRI 2 4867 6975 BsgI 8 671 1030 2686 2896 3109 3229 3697 4669 Bsh1365I 1 3101 BsiMI 1 6122 BsiWI 1 4541 BsmI 1 7068 BsmBI 3 2397 3337 5735 Bsp13I 1 6122 Bsp19I 3 5506 5896 6066 Bsp68I 1 330 Bsp106I 1 2594 Bsp119I 2 4850 6522 Bsp120I 1 1088 Bsp1407I 1 6176 Bsp1720I 1 4783 BspCI 5 1893 2619 3099 3552 8549 BspDI 1 2594 BspEI 1 6122 BspHI 2 34 8006 BspLU11I 3 1108 4529 7297 BspMI 3 999 2544 4416 BspTI 1 854 BspXI 1 2594 BsrBI 9 40 1264 1332 2375 4273 4437 4834 6654 7230 BsrBRI 1 3101 BsrDI 2 8240 8414 BsrFI 10 553 1175 3057 4789 4900 6673 6714 6872 6933 8259 BsrGI 1 6176 BssAI 10 553 1175 3057 4789 4900 6673 6714 6872 6933 8259 BssHII 2 3268 6636 BssSI 5 468 872 2827 7459 8843 BssT1I 4 1559 5506 5896 6066 Bst98I 1 854 Bst1107I 2 4535 6350 BstBI 2 4850 6522 Bst2BI 5 468 872 2827 7459 8843 BstEII 1 4840 BstPI 1 4840 BstSFI 9 787 1292 1682 1756 2158 4641 7551 7742 8420 BstSNI 1 5486 BstXI 3 1747 3990 4607 BstZI 3 4829 5801 6931 Bsu15I 1 2594 Bsu36I 1 1994 CbiI 2 4850 6522 CciNI 1 4829 CelII 1 4783 Cfr9I 1 6688 Cfr10I 10 553 1175 3057 4789 4900 6673 6714 6872 6933 8259 Cfr42I 3 729 1076 5801 ClaI 1 2594 CpoI 1 5825 CspI 1 5825 Csp45I 2 4850 6522 CvnI 1 1994 DraI 7 413 976 4930 6290 8045 8064 8756 DraII 3 1088 1125 1364 DraIII 3 1511 2960 6953 DrdI 3 3879 6552 7394 EagI 3 4829 5801 6931 Eam1104I 3 1871 7183 8974 Eam1105I 1 8179 EarI 3 1871 7183 8974 Ecl136II 4 1301 1723 3708 5712 EclHKI 1 8179 EclXI 3 4829 5801 6931 Eco24I 8 864 1092 1303 1725 3710 4759 5170 5714 Eco31I 3 1683 4888 8240 Eco32I 1 2883 Eco47III 1 3605 Eco52I 3 4829 5801 6931 Eco57I 5 1544 2504 6250 7834 8846 Eco81I 1 1994 Eco91I 1 4840 Eco105I 1 5486 Eco130I 4 1559 5506 5896 6066 Eco147I 2 846 889 Eco255I 1 8659 EcoICRI 4 1301 1723 3708 5712 EcoNI 2 564 1292 EcoO65I 1 4840 EcoO109I 3 1088 1125 1364 EcoRI 2 1751 4774 EcoRV 1 2883 EcoT14I 4 1559 5506 5896 6066 EcoT38I 8 864 1092 1303 1725 3710 4759 5170 5714 EheI 1 1415 ErhI 4 1559 5506 5896 6066 Esp3I 3 2397 3337 5735 Esp1396I 2 3015 3381 FauNDI 3 4727 5380 6130 FbaI 2 3116 4939 FriOI 8 864 1092 1303 1725 3710 4759 5170 5714 FseI 2 1179 6876 FspI 2 1912 8401 GsuI 7 1999 2497 3359 4773 5759 5947 8249 HincII 9 122 156 356 2196 2820 4648 5834 6505 6610 HindII 9 122 156 356 2196 2820 4648 5834 6505 6610 HindIII 3 387 1709 6021 HpaI 2 2196 2820 KasI 1 1413 KpnI 2 1719 1954 Kpn2I 1 6122 KspI 3 729 1076 5801 Ksp22I 2 3116 4939 Ksp632I 3 1871 7183 8974 LspI 2 4850 6522 MamI 1 3101 MfeI 3 283 546 6460 MluI 6 350 3072 3852 4277 4894 6222 MluNI 1 6071 MroI 1 6122 MroNI 3 1175 6872 6933 MscI 1 6071 MslI 8 3110 3667 4059 4605 5511 8431 8590 8949 MspCI 1 854 MunI 3 283 546 6460 Mva1269I 1 7068 NaeI 3 1177 6874 6935 NarI 1 1414 NcoI 3 5506 5896 6066 NdeI 3 4727 5380 6130 NgoAIV 3 1175 6872 6933 NgoMI 3 1175 6872 6933 NheI 1 5901 NotI 1 4829 NruI 1 330 NspI 4 1112 4533 5152 7301 NspV 2 4850 6522 PaeI 1 5152 PaeR7I 3 1440 4835 6321 PauI 2 3268 6636 Pfl23II 1 4541 PflMI 2 3015 3381 PinAI 2 553 4900 PleI 9 151 1350 1390 1670 4741 5210 5550 7665 8168 Ple19I 5 1893 2619 3099 3552 8549 PmeI 1 4930 Pme55I 2 846 889 PshBI 3 5840 7129 8351 Psp1406I 3 668 8405 8778 PspAI 1 6688 PspALI 1 6690 Psp124BI 4 1303 1725 3710 5714 PspEI 1 4840 PspLI 1 4541 PspOMI 1 1088 PstI 3 791 1296 1760 PstNHI 1 5901 PvuI 5 1893 2619 3099 3552 8549 PvuII 3 1862 4419 4782 RcaI 2 34 8006 RsrII 1 5825 SacI 4 1303 1725 3710 5714 SacII 3 729 1076 5801 SalI 3 120 154 6503 SapI 1 7183 ScaI 1 8659 SexAI 1 6763 SfcI 9 787 1292 1682 1756 2158 4641 7551 7742 8420 Sfr274I 3 1440 4835 6321 Sfr303I 3 729 1076 5801 SfuI 2 4850 6522 SgrAI 1 6714 SmaI 1 6690 SnaBI 1 5486 SpeI 1 1734 SphI 1 5152 SplI 1 4541 SseBI 2 846 889 SspI 2 3 3000 SspBI 1 6176 SstI 4 1303 1725 3710 5714 SstII 3 729 1076 5801 StuI 2 846 889 StyI 4 1559 5506 5896 6066 SunI 1 4541 Van91I 2 3015 3381 Vha464I 1 854 VneI 5 4115 4230 6945 7600 8846 VspI 3 5840 7129 8351 XcmI 2 3987 4732 XhoI 3 1440 4835 6321 XmaI 1 6688 XmaIII 3 4829 5801 6931 XmaCI 1 6688 XmnI 2 774 8778 The enzymes listed below do not restrict pTracerª-EF/lacZ AscI AspI AtsI AvrII BbrPI BlnI Eco72I EcoT22I Mph1103I NsiI PacI PmaCI PmlI Ppu10I PpuMI PshAI Psp5II SbfI SfiI SgfI SmiI SrfI Sse8387I SwaI Tth111I XbaI Zsp2I